DUS2L (DUS2) (NM_017803) Human Untagged Clone

SKU SC113958

DUS2L (untagged)-Human dihydrouridine synthase 2-like, SMM1 homolog (S. cerevisiae) (DUS2L)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DUS2L
• Synonyms: DUS2L; SMM1; URLC8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113958 sequence for NM_017803 edited (data generated by NextGen Sequencing); ATGATTTTGAATAGCCTCTCTCTGTGTTACCATAATAAGCTAATCCTGGCCCCAATGGTT; CGGGTAGGGACTCTTCCAATGAGGCTGCTGGCCCTGGATTATGGAGCGGACATTGTTTAC; TGTGAGGAGCTGATCGACCTCAAGATGATTCAGTGCAAGAGAGTTGTTAATGAGGTGCTC; AGCACAGTGGACTTTGTCGCCCCTGATGATCGAGTTGTCTTCCGCACCTGTGAAAGAGAG; CAGAACAGGGTGGTCTTCCAGATGGGGACTTCGGACGCAGAGCGAGCCCTTGCTGTGGCC; AGGCTTGTAGAAAATGATGTGGCTGGTATTGATGTCAACATGGGCTGTCCAAAACAATAT; TCCACCAAGGGAGGAATGGGAGCTGCCCTGCTGTCAGACCCTGACAAGATTGAGAAGATC; CTCAGCACTCTTGTTAAAGGGACACGCAGACCTGTGACCTGCAAGATTCGCATCCTGCCA; TCGCTAGAAGATACCCTGAGCCTTGTGAAGCGGATAGAGAGGACTGGCATTGCTGCCATC; GCAGTTCATGGGAGGAAGCGGGAGGAGCGACCTCAGCATCCTGTCAGCTGTGAAGTCATC; AAAGCCATTGCTGATACCCTCTCCATTCCTGTCATAGCCAACGGAGGATCTCATGACCAC; ATCCAACAGTATTCGGACATAGAGGACTTTCGACAAGCCACGGCAGCCTCTTCCGTGATG; GTGGCCCGAGCAGCCATGTGGAANNCATCTATCTTCCTCAAGGAGGGTCTGCGGCCCCTG; GAGGAGGTCATGCAGAAATACATCAGATACGCGGTGCAGTATGACAACCACTACACCAAC; ACCAAGTACTGCTTGTGCCAGATGCTACGAGAACAGCTGGAGTCGCCCCAGGGAAGGTTG; CTCCATGCTGCCCAGTCTTCCCGGGAAATTTGTGAGGCCTTTGGCCTTGGTGCCTTCTAT; GAGGAGACCACACAGGAGCTGGATGCCCAGCAGGCCAGGCTCTCAGCCAAGACTTCAGAG; CAGACAGGGGAGCCAGCTGAAGATACCTCTGGTGTCATTAAGATGGCTGTCAAGTTTGAC; CGGAGAGCATACCCAGCCCAGATCACCCCTAAGATGTGCCTACTAGAGTGGTGCCGGAGG; GAGAAGTTGGCACAGCCTGTGTATGAAACGGTTCAACGCCCTCTAGATCGCCTGTTCTCC; TCTATTGTCACCGTTGCTGAACAAAAGTATCAGTCTACCTTGTGGGACAAGTCCAAGAAA; CTGGCGGAGCAGGCTGCAGCCATCGTCTGTCTGCGGAGCCAGGGCCTCCCTGAGGGTCGG; CTGGGTGAGGAGAGCCCTTCCTTGCACAAGCGAAAGAGGGAGGCTCCTGACCAAGACCCT; GGGGGCCCCAGAGCTCAGGAGCTAGCACAACCTGGGGATCTGTGCAAGAAGCCCTTTGTG; GCCTTGGGAAGTGGTGAAGAAAGCCCCCTGGAAGGCTGGTGA; ; Clone variation with respect to NM_017803.3; 273 a=>g;744 c=>n;745 c=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017803 unedited; ACATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGGCACGAGGGTACGGTGTGTG; GAGCTGGAGCACCGTGAGGAAGAAGCGAGGTTCTTTTTAAGAGTTCAGCTGCGAGATATC; AAACAAAGAATTACTCTGTACAAAGCCAGAACACATATATCAAAGTAATCCTGAAGTATC; AGAACAAAATAATAGGCTGTAACAGAGGAGGAAATGATTTTGAATAGCCTCTCTCTGTGT; TACCATAATAAGCTAATCCTGGCCCCAATGGTTCGGGTAGGGACTCTTCCAATGAGGCTG; CTGGCCCTGGATTATGGAGCGGACATTGTTTACTGTGAGGAGCTGATCGACCTCAAGATG; ATTCAGTGCAAGAGAGTTGTTAATGAGGTGCTCAGCACAGTGGACTTTGTCGCCCCTGAT; GATCGAGTTGTCTTCCGCACCTGTGAAAGAGAGCAGAACAGGGTGGTCTTCCAGATGGGG; ACTTCGGACGCAGAGCGAGCCCTTGCTGTGGCCAGGCTTGTAGAAAATGATGTGGCTGGT; ATTGATGTCAACATGGGCTGTCCAAAACAATATTCCACCAAGGGAGGAATGGGAGCTGCC; CTGCTGTCAGACCCTGACAAGATTGAGAAGATCCTCAGCACTCTTGTTAAAGGGACACGC; AGACCTGTGACCTGCAAGATTCGCATCCTGCCATCGCTAGAAGAACCCTGAGCCTTGTGA; AGCGGATAGAGAGGACTGGCATTGCTGCCATCGCAGNTCATGGGAGGAAAGCGGAGGAGC; GACCTCACCATCCTGTCAGCTGTGAAGTCATCAAAGCCATTGCTGATACCCTCTCCATTC; CTGTCATAGCCAACGGAGGATCTCATGACCACATTCCACAGTATTCGGACATAGAGGACT; TTCGACAGCCACGGCAGCCTCTTNCGTGATGGTGGCCCGAGCAGCCATGTTGAACCATCT; ATCTTCTN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017803 unedited; TCTATGAACCGCAGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTATGAAGATG; CCCAAGGCTGAAGCAGCCAGCCGTGGGTGAGGGTAGGGGGTGTCTTTATTGCACAGTACC; AATGTCAGGTTGGGGAGTTACCACCTGAAAAGCTCCTGTTTTCAAAGAGAACTTGACCCT; GTCACTCAGATCCACCAAAGGGGCCAGGATCCACCCCTGGGCGGCCCCTGGTCCATGCTC; TAGGCTGATGGCCCCTGCCAGGACATCTAACTTCTGCCAGGCAAGACCAGCAAACTGTTC; AACGGCATCTGGTTCATGCTGTGGCATCCACAGCCACCTTAGCACCAGGCCCATGGAGGG; GTGACTAAGGCAGGAAGAGTAGTCACCAGCCTTCCAGGGGGCTTTCTTCACCACTTCCCA; AGGCCACAAAGGGCTTCTTGCACAGATCCCCAGGTTGTGCTAGCTCCTGAGCTCTGGGGC; CCCCAGGGTCTTGGTCAGGAGCCTCCCTCTTTCGCTTGTGCAAGGAAGGGCTCTCCTCAC; CCAGCCGACCCTCAGGGAGGCCCTGGCTCCGCAGACAGACGATGGCTGCAGCCTGCTCCG; CCAGTTTCTTGGACTTGTCCCACAAGGTAGACTGATACTTTTGTTCAGCAACGGTGACAA; TAGAGGAGAACAGGCGATCTAGAGGGCGTTGAACCGTTTCATACACAGGCTGTGCCAACT; TCTCCCTCCGGCACCACTCTAGTAAGCACATCTTANGGGTGATCTGGGCTGGGTATGCTC; TCCGGTCAAACTTGACAGCCATCTTAATGACACCAGAGGTATCTTCAGCTGGCTCCCCTG; TCTGCTCTGAAATCTTGGCTGAAAGCCTTGGCTGCTGGGCATCCAGCTTCTGTGTGGGTC; TCTCATAGAAGCACCAAGGCCAAAGCCCTACAATTTCCCGGNAAGACTGGCANCATGGAG; CAACCTTCCTTGGGCGACTCCGCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_017803
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017803.3, NP_060273.1
• RefSeq Size: 1999 bp
• RefSeq ORF: 1482 bp
• Locus ID: 54920
• UniProt ID: Q9NX74
• Cytogenetics: 16q22.1
• Domains: DSRM, Dus
• Summary: This gene encodes a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. The resulting modified bases confer enhanced regional flexibility to tRNA. The encoded protein may increase the rate of translation by inhibiting an interferon-induced protein kinase. This gene has been implicated in pulmonary carcinogenesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).