SCYL2 (NM_017988) Human Untagged Clone

SKU SC113782

SCYL2 (untagged)-Human SCY1-like 2 (S. cerevisiae) (SCYL2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SCYL2
• Synonyms: AMC4; AMCNACC; CVAK104
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113782 sequence for NM_017988 edited (data generated by NextGen Sequencing); ATGGAGTCCATGCTTAATAAATTGAAGAGTACTGTTACAAAAGTAACAGCTGATGTCACT; AGTGCTGTAATGGGAAATCCTGTCACTAGAGAATTTGATGTTGGTCGACACATTGCCAGT; GGTGGCAATGGGCTAGCTTGGAAGATTTTTAATGGCACAAAAAAGTCAACAAAGCAGGAA; GTGGCAGTTTTTGTCTTTGATAAAAAACTGATTGACAAGTATCAAAAATTTGAAAAGGAT; CAAATCATTGATTCTCTAAAACGAGGAGTCCAACAGTTAACTCGGCTTCGACACCCTCGA; CTTCTTACTGTCCAGCATCCTTTAGAAGAATCCAGGGATTGCTTGGCATTTTGTACAGAA; CCAGTTTTTGCCAGTTTAGCCAATGTTCTTGGTAACTGGGAAAATCTACCTTCCCCTATA; TCTCCAGACATTAAGGATTATAAACTTTATGATGTAGAAACCAAATATGGTTTGCTTCAG; GTTTCTGAAGGATTGTCATTCTTGCATAGCAGTGTGAAAATGGTGCATGGAAATATCACT; CCTGAAAATATAATTTTGAATAAAAGTGGAGCCTGGAAAATAATGGGTTTTGATTTTTGT; GTATCATCAACCAATCCTTCTGAACAAGAGCCTAAATTTCCTTGTAAAGAATGGGACCCA; AATTTACCTTCATTGTGTCTTCCAAATCCTGAATATTTGGCTCCTGAATACATACTTTCT; GTGAGCTGTGAAACAGCCAGTGATATGTATTCTTTAGGAACTGTTATGTATGCTGTATTT; AATAAAGGGAAACCTATATTTGAAGTCAACAAGCAAGATATTTACAAGAGTTTCAGTAGG; CAGTTGGATCAGTTGAGTCGTTTAGGATCTAGTTCACTTACAAATATACCTGAGGAAGTT; CGTGAACATGTAAAGCTACTGTTAAATGTAACTCCGACTGTAAGACCAGATGCAGATCAA; ATGACAAAGATTCCCTTCTTTGATGATGTTGGTGCAGTAACACTGCAATATTTTGATACC; TTATTCCAAAGAGATAATCTTCAGAAATCACAGTTTTTCAAAGGACTGCCAAAGGTTCTA; CCAAAACTGCCCAAGCGTGTCATTGTGCAGAGAATTTTGCCTTGTTTGACTTCAGAATTT; GTAAACCCTGACATGGTACCTTTTGTTTTGCCCAATGTTYTACTTATTGCTGAGGAATGC; ACCAAAGAAGAATATGTCAAATTAATTCTTCCTGAACTTGGCCCTGTGTTTAAGCAGCAG; GAGCCAATCCAGATTTTGTTAATTTTCCTACAAAAAATGGATTTGCTACTAACCAAAACC; CCTCCTGATGAGATAAAGAACAGTGTTCTACCCATGGTTTACAGAGCACTAGAAGCTCCT; TCCATTCAGATCCAGGAGCTCTGTCTAAACATCATTCCAACCTTTGCAAATCTTATAGAC; TACCCATCCATGAAAAACGCTTTGATACCAAGAATTAAAAATGCTTGTCTACAAACATCT; TCCCTTGCGGTTCGTGTAAATTCATTAGTGTGCTTAGGAAAGATTTTGGAATACTTGGAT; AAGTGGTTTGTACTTGATGATATCCTACCCTTCTTACAACAAATTCCATCCAAGGAACCT; GCGGTCCTCATGGGAATTTTAGGTATTTACAAATGTACTTTTACTCATAAGAAGTTGGGA; ATCACCAAAGAGCAGCTGGCCGGAAAAGTGTTGCCTCATCTTATTCCCCTGAGTATTGAA; AACAATCTTAATCTTAATCAGTTCAATTCTTTCATTTCCGTCATAAAAGAAATGCTTAAT; AGATTGGAGTCTGAACATAAGACTAAACTGGAGCAACTTCATATAATGCAAGAACAGCAG; AAATCTTTGGATATAGGAAATCAAATGAATGTTTCTGAGGAGATGAAAGTTACAAATATT; GGGAATCAGCAAATTGACAAAGTTTTTAACAACATTGGAGCAGACCTTCTGACTGGCAGT; GAGTCCGAAAATAAAGAGGACGGGTTACAGAATAAACATAAAAGAGCATCACTTACACTT; GAAGAAAAACAAAAATTAGCAAAAGAACAAGAGCAGGCACAGAAGCTGAAAAGCCAGCAG; CCTCTTAAACCCCAAGTGCACACACCTGTTGCTACTGTTAAACAGACTAAGGACTTGACA; GACACACTGATGGATAATATGTCATCCTTGACCAGCCTTTCTGTTAGTACCCCTAAATCT; TCTGCTTCAAGTACTTTCACTTCTGTTCCTTCCATGGGCATTGGTATGATGTTTTCTACA; CCAACTGATAATACAAAGAGAAATTTGACAAATGGCCTAAATGCCAATATGGGCTTTCAG; ACTTCAGGATTCAACATGCCCGTTAATACAAACCAGAACTTCTACAGTAGTCCAAGCACA; GTTGGAGTGACCAAGATGACTCTGGGAACACCTCCCACTTTGCCAAACTTCAATGCTTTG; AGTGTTCCTCCTGCTGGTGCAAAGCAGACCCAACAAAGACCCACAGATATGTCTGCCCTT; AATAATCTCTTTGGCCCTCAGAAACCCAAAGTTAGCATGAACCAGTTATCACAACAGAAA; CCAAATCAGTGGCTTAATCAGTTTGTACCTCCTCAAGGTTCTCCAACTATGGGCAGTTCA; GTAATGGGGACACAGATGAACGTGATAGGACAATCTGCTTTTGGTATGCAGGGTAATCCT; TTCTTTAACCCACAGAACTTTGCACAGCCACCAACTACTATGACCAATAGCAGTTCAGCT; AGCAATGATTTAAAAGATCTTTTTGGGTGA; ; Clone variation with respect to NM_017988.4; 1180 c=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017988 unedited; CTATAGGGGCGGCCGCGATTCGGCACGAGGCCCCTCCCCACCCCTTTCCTTCTAGCTCCG; ACGTTTGCGGCCGCGGGGGCGGCGGAGGATATGGAGTAAAGCCAGAGTCAGTGGCCAGGC; ACGAAGGCAGAGCAGGAACAGCCAGGAGGCGTTTATTAGGGGGGCGGGGGGAAAGAGCCC; CAGCACCGCCCCTCCTGGAAGAAGGAAGAGGTAACTATAACTACCCAATATTGCAGCCAT; GGAGTCCATGCTTAATAAATTGAAGAGTACTGTTACAAAAGTAACAGCTGATGTCACTAG; TGCTGTAATGGGAAATCCTGTCACTAGAGAATTTGATGTTGGTCGACACATTGCCAGTGG; TGGCAATGGGCTAGCTTGGAAGATTTTTAATGGCACAAAAAAGTCAACAAAGCAGGAAGT; GGCAGTTTTTGTCTTTGATAAAAAACTGATTGACAAGTATCAAAAATTTGAAAAGGATCA; AATCATTGATTCTCTAAAACGAGGAGTCCAACAGTTAACTCGGCTTCGACACCCTCGACT; TCTTACTGTCCAGCATCCTTTAGAAGAATCCAGGGATTGCTTGGCATTNTGTACAGAACC; AGTTTTTGCCAGTTTAGCCAATGTTCTTGGTAACTGGGAAAATCTACCTTCCCCTATATC; TCCAGACATTAAGGATTATAAACTTTATGATGTAGAAACCAAATATGGGTTGCTTCAGGT; TTCTGAAGGATTGTCATTCTTGCATAGCAGTGTGAAAATGGTGCATGGAAATATCACTCC; TGAAAATATATTTTGAATANAGTGGAGCCTGAANATAATGGGGTTTTGATTTTGTGTATC; ATCACCAATCCTTCTGACAGAGCCTAAATTCCTTGTAAGAATGGACCAAATTACCTTCAT; GGGTCTCCAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017988 unedited; TCCTCTGCCGCACTCNANAGTTGAGTTTATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTGAAAGGTTACTCCTAAATATAGTTTAATTCTTACAAATCTTC; TTTTGAAAAGGCAATTCATATTTGCTGCACCCCCAAAAGTTTGAATTTGAAATGAAATAT; GAAGGGAGTAGTCAGGGAAGTCACATCATTTTGCCTTGGCAAATTTCCAAACAAATGGGG; CCATCCCTTTTCCTTGTTACGGGGGGAAAAAAGGGTTTTTAAATTTTTCCAAGGGAATGC; CAAAAAATCCTTTTCTTTTTGGGGCCAACCCTGCTAAAATATAATTTACAAGGATTAGTT; TGCCCTTAAAAGGGTTAAAAACCCTTTTAACCCCATGAAATGAAGGTTAAGTTGAATTTT; GGAGTATTTGCTCAGTCTTTGGCCTAAACAATAGTTCATTTGAAAAGTTTGGAAAAAGCA; AATAACCGGGAACTTCTCTTTATGCTTATAATTTTCCCACGGGCATCTTAAAGGCCAACA; AATCAATCCCGCTTCAAGATTTTTTCCCTTTTAAAATGAAGACTAATGCCTCTTAGACTG; GGTCCCATATAGTACTTAAAAGATGAGCTTGGAAGGACCCTCCCCTCATTTTTTAAATAC; CCCGAGTTCCCCAGGCAAAAAAAACTTTGGTTTAAAAGTTTTGTTATTCAGAAACCCCGG; TTCCTTTTTCTTTTGAAATGGGAAGGGCTGGTCTTAAAAACCGAAAGAGTTGGAAGAGAA; AAAACCTCAGCTTAAAAACTCCCTGGTAAAGTCTACACTCTTGCTGCCTGGCAAAAGTTT; CTGGCCAAATGTACTTTCCATAAAATCTCCCCCGCCCCTTTTAGAAGGAAACCCCCCCCT; GTTGAACCGAAAACCTTCAAAAAAGAAACCTTCCCCAAATTTTAATT;
• Restriction Sites: NotI-NotI
• ACCN: NM_017988
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017988.4, NP_060458.3
• RefSeq Size: 3818 bp
• RefSeq ORF: 2790 bp
• Locus ID: 55681
• UniProt ID: Q6P3W7
• Cytogenetics: 12q23.1
• Protein Families: Druggable Genome, Protein Kinase
• Summary: The protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2015]; Transcript Variant: This variant (1) encodes isoform 1. Both variants 1 and 2 encode the same protein.