STEAP3 (NM_018234) Human Untagged Clone

SKU SC113663

STEAP3 (untagged)-Human STEAP family member 3 (STEAP3), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: STEAP3
• Synonyms: AHMIO2; dudlin-2; dudulin-2; pHyde; STMP3; TSAP6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113663 sequence for NM_018234 edited (data generated by NextGen Sequencing); ATGCCAGAAGAGATGGACAAGCCACTGATCAGCCTCCACCTGGTGGACAGCGATAGTAGC; CTTGCCAAGGTCCCCGATGAGGCCCCCAAAGTGGGCATCCTGGGTAGCGGGGACTTTGCC; CGCTCCCTGGCCACACGCCTGGTGGGCTCTGGCTTCAAAGTGGTGGTGGGGAGCCGCAAC; CCCAAACGCACAGCCAGGCTGTTTCCCTCAGCGGCCCAAGTGACTTTCCAAGAGGAGGCA; GTGAGCTCCCCGGAGGTCATCTTTGTGGCTGTGTTCCGGGAGCACTACTCTTCACTGTGC; AGTCTCAGTGACCAGCTGGCGGGCAAGATCCTGGTGGATGTGAGCAACCCTACAGAGCAA; GAGCACCTTCAGCATCGTGAGTCCAATGCTGAGTACCTGGCCTCCCTCTTCCCCACTTGC; ACAGTGGTCAAGGCCTTCAATGTCATCTCTGCCTGGACCCTGCAGGCTGGCCCAAGGGAT; GGTAACAGGCAGGTGCCCATCTGCGGTGACCAGCCAGAAGCCAAGCGTGCTGTCTCGGAG; ATGGCGCTCGCCATGGGCTTCATGCCCGTGGACATGGGATCCCTGGCGTCAGCCTGGGAG; GTGGAGGCCATGCCCCTGCGCCTCCTCCCGGCCTGGAAGGTGCCCACCCTGCTGGCCCTG; GGGCTCTTCGTCTGCTTCTATGCCTACAACTTCGTCCGGGACGTTCTGCAGCCCTATGTG; CAGGAAAGCCAGAACAAGTTCTTCAAGCTGCCCGTGTCCGTGGTCAACACCACACTGCCG; TGCGTGGCCTACGTGCTGCTGTCACTCGTGTACTTGCCCGGCGTGCTGGCGGCTGCCCTG; CAGCTGCGGCGCGGCACCAAGTACCAGCGCTTCCCCGACTGGCTGGACCACTGGCTACAG; CACCGCAAGCAGATCGGGCTGCTCAGCTTCTTCTGCGCCGCCCTGCACGCCCTCTACAGC; TTCTGCTTGCCGCTGCGCCGCGCCCACCGCTACGACCTGGTCAACCTGGCAGTCAAGCAG; GTCTTGGCCAACAAGAGCCACCTCTGGGTGGAGGAGGAGGTCTGGCGGATGGAGATCTAC; CTCTCCCTGGGAGTGCTGGCCCTCGGCACGTTGTCCCTGCTGGCCGTGACCTCACTGCCG; TCCATTGCAAACTCGCTCAACTGGAGGGAGTTCAGCTTCGTTCAGTCCTCACTGGGCTTT; GTGGCCCTCGTGCTGAGCACACTGCACACGCTCACCTACGGCTGGACCCGCGCCTTCGAG; GAGAGCCGCTACAAGTTCTACCTGCCTCCCACCTTCACGCTCACGCTGCTGGTGCCCTGC; GTCGTCATCCTGGCCAAAGCCCTGTTTCTCCTGCCCTGCATCAGCCGCAGACTCGCCAGG; ATCCGGAGAGGCTGGGAGAGGGAGAGCACCATCAAGTTCACGCTGCCCACAGACCACGCC; CTGGCCGAGAAGACGAGCCACGTATGA; ; Clone variation with respect to NM_018234.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018234 unedited; TCAAATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACCAGGTTCAAGCGATTC; TCCTGCTTCAGCCTCCGGAGTAGCTGGGATTACAGGCACGTGCCAACACACCCAGCCACC; AAAATGCCAGAAGAGATGGACAAGCCACTGATCAGCCTCCACCTGGTGGACAGCGATAGT; AGCCTTGCCAAGGTCCCCGATGAGGCCCCCAAAGTGGGCATCCTGGGTAGCGGGGACTTT; GCCCGCTCCCTGGCCACACGCCTGGTGGGCTCTGGCTTCAAAGTGGTGGTGGGGAGCCGC; AACCCCAAACGCACAGCCAGGCTGTTTCCCTCAGCGGCCCAAGTGACTTTCCAAGAGGAG; GCAGTGAGCTCCCCGGAGGTCATCTTTGTGGCTGTGTTCCGGGAGCACTACTCTTCACTG; TGCAGTCTCAGTGACCAGCTGGCGGGCAAGATCCTGGTGGATGTGAGCAACCCTACAGAG; CAAGAGCACCTTCAGCATCGTGAGTCCAATGCTGAGTACCTGGCCTCCCTCTTCCCCACT; TGCACAGTGGTCAAGGCCTTCAATGTCATCTCTGCCTGGACCCTGCAGGCTGGCCCAAGG; GATGGTAACAGGCAGGTGCCCATCTGCGGTGACCAGCCAGAAGCCAAGCGTGCTGTCTCG; GAGATGGCGCTCGCCATGGGCTTCATGCCCGTGGACATGGGATCCCTGGCGTCAGCCTGG; GAGGTGGAGGCCATGCCCCTGCGCCTCCTCCCGGCCTGGAAGGTGCCCACCCTGCTGGCC; CTGGGGCTCTTCGTCTGCTTCTATGCCTACAACTTCGTCCGGGACGTTCTGCAGCCCTAT; GTGCAGGAAAGCCAGGACAAGTTCTTTCAGCTTGCCGTGTCCGTGGTCACACCACACTGC; CGTGCGTGGCCTACTGCTGCTGTCACTGTGTACTTGCCCGGGTGCTGTCGCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018234 unedited; GCCGCGGCCGCTATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAAGGGGAGGGGGTTATTTGC; CCCGGTTTTATACCCGCTACCCGGGTAAAAAAAAAAAAAAAAAACTTTTTTGAAGGGCTA; AATTTTAAAATTCAACCCCAAACGGGGGGATTTGGGATTTCCAAAACAGGGGGGGGGGGG; CCTTTTGGCCCCCAAGGGCCCGGGGATTTACCCGGCTGGGGAAAAAAAGGGCTCTCGGGA; AGCTGGGGCCCAAACCCCGGGGGGGGGGGGAAAACAACAGGGGGCCCCCAATTAGGGCCC; AAAACACAACCCCCCTGCCTTTTGGGGGAATGGCCAAAAGGGGGGACCTGTTCCCTTCTG; CCACCCCCCCTATAAAGGGTTCCCTTTTGAGCACCTTTAACCAAAAGGGGGGGGGAAGGG; GAGGACCTCCCCTGGGTTTTGCTTTGGGCCAACTCCTTTGGTTTAAGGAGCCATTCCCTG; GCCCGGGGAGGGAGCCAATACCCACCGGGGTTGGGGAACATTGGGGGGGTTCCATTGGCC; CGGCTTCCCGTGCCTTCATTAAAAGGAAACAAGGGCCCCTTGGTTTGCCCAGGAATGGTA; AAATTTTGTTTTATCTGGCCCCTTTAAAAAGGGTTTTTTGGGGCCCACCTGGAGCCTCCA; TAAGGGCCATTATGGAAAAGAGTTTGCCGCTAACCTTGGCCAACCAAGGAAATACCCCGG; GAAAGCTCCCCGGATGGCCTCCCCGCATGGGGATGGGCCCAAGAACTACCTTGAGGGGGT; TTCCCCCGTTTTACTGAAAACCCTATGGCTGATAAAACGAGCCTGAAACTTCTGGGGGGA; GGGGCAGGGCACCTCATTGGAATTCTTTGGAACCTTATGGGCTACCTTTCTTTATAAAAG; GTTTGGCCGGAACAAGAAACCACCTCTGCTTAAAACTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_018234
• Insert Size: 4230 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018234.2, NP_060704.2
• RefSeq Size: 3938 bp
• RefSeq ORF: 1467 bp
• Locus ID: 55240
• UniProt ID: Q658P3
• Cytogenetics: 2q14.2
• Domains: F420_oxidored
• Protein Families: Transmembrane
• Protein Pathways: p53 signaling pathway
• Summary: This gene encodes a multipass membrane protein that functions as an iron transporter. The encoded protein can reduce both iron (Fe3+) and copper (Cu2+) cations. This protein may mediate downstream responses to p53, including promoting apoptosis. Deficiency in this gene can cause anemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]; Transcript Variant: This variant (2) lacks an exon and contains an alternate exon in the 5' region, and initiates translation at a downstream start codon compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.