Phytoceramidase (ACER3) (NM_018367) Human Untagged Clone

SKU SC113575

ACER3 (untagged)-Human alkaline ceramidase 3 (ACER3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Phytoceramidase
• Synonyms: APHC; PHCA; PLDECO
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018367, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCTCCGGCCGCGGACCGAGAGGGCTACTGGGGCCCCACGACCTCCACGCTGGACTGGTGCGAGGAGA; ACTACTCCGTGACCTGGTACATCGCCGAGTTCTGGAATACAGTGAGTAACCTGATCATGATTATACCTCC; AATGTTCGGTGCAGTTCAGAGTGTTAGAGACGGTCTGGAAAAGCGGTACATTGCTTCTTATTTAGCACTC; ACAGTGGTAGGAATGGGATCCTGGTGCTTCCACATGACTCTGAAATATGAAATGCAGCTATTGGATGAAC; TCCCAATGATATACAGCTGTTGCATATTTGTGTACTGCATGTTTGAATGTTTCAAGATCAAGAACTCAGT; AAACTACCATCTGCTTTTTACCTTAGTTCTATTCAGTTTAATAGTAACCACAGTTTACCTTAAGGTAAAA; GAGCCGATATTCCATCAGGTCATGTATGGAATGTTGGTCTTTACATTAGTACTTCGATCTATTTATATTG; TTACATGGGTTTATCCATGGCTTAGAGGACTGGGTTATACATCATTGGGTATATTTTTATTGGGATTTTT; ATTTTGGAATATAGATAACATATTTTGTGAGTCACTGAGGAACTTTCGAAAGAAGGTACCACCTATCATA; GGTATTACCACACAATTTCATGCATGGTGGCATATTTTAACTGGCCTTGGTTCCTATCTTCACATCCTTT; TCAGTTTGTATACAAGAACACTTTACCTGAGATATAGGCCAAAAGTGAAGTTTCTCTTTGGAATCTGGCC; AGTGATCCTGTTTGAGCCTCTCAGGAAGCATTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018367 unedited; TATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCCGGGCGGGGCGCGAGCGA; GCGCGCGGGCTGGGCCGGAGCCGGCCTGGTCGCCAGCCTAACCCGGCACAGTGAGCGGAG; CGCCTGGGCGGCGGCGGCGGCGGCGTGATGGCTCCGGCCGCGGACCGAGAGGGCTACTGG; GGCCCCACGACCTCCACGCTGGACTGGTGCGAGGAGAACTACTCCGTGACCTGGGAATAC; AGTGAGTAACCTGATCATGATTATACCTCCAATGTTCGGTGCAATTCAGAGTGTTAGAGA; CGGTCTGGAAAAACGGTACATTGCTTCTTATTTAGCACTCACAGTGGTAGGAATGGGATC; CTGGTGCTTCCACATGACTCTGAAATATGAAATGCAGCTATTGGATGAACTCCCAATGAT; ATACAGCTGTTGCATATTTGTGTACTGCATGTTTGAATGTTTCAAGATCAAGAACTCAGT; AAACTACCATCTGCTTTTTACCTTAGTTCTATTCAGTTTAATAGTAACCACAGTTTACCT; TAAGGTAAAAGAGCCAATATTCCATCAGAAGAGTATTGCTTTGAACTGCCCCTGAGAGCT; GAAGATTGGCCATGGNGTTTATCCATGGCTTAGAGGACTGGGTTATACATCATTGNGTAT; ATTNTTATTGGNGATTTTTATTTTGGAATATAGATAACATATTTTGTGAGTCACTGAGGA; ACTTTCGAAAGAAGTACCACCTATCATAGGTATTACCACACAATTTCATGCATGGTGGCA; TATTTTAACTGGNCCCTGGNTNCCTATCTTACATCCTTTTNCAGNTTGTATCCAAGAACA; CTTTACCTGAGAATAGGCANAAANGGGAAGTTCTCTTTGGATCTGGCCAAGGGATCCTGT; TTGAGCCTTTCAGAGCATTGATGAATCATTCCACCAGAANACAAACAGCCCCTACATAGA; ACTGGCAGAATAAATAGGAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_018367
• Insert Size: 5310 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018367.3, NP_060837.2
• RefSeq Size: 3473 bp
• RefSeq ORF: 804 bp
• Locus ID: 55331
• UniProt ID: Q9NUN7
• Cytogenetics: 11q13.5
• Protein Families: Transmembrane
• Protein Pathways: Sphingolipid metabolism
• Summary: Endoplasmic reticulum and Golgi ceramidase that catalyzes the hydrolysis of unsaturated long-chain C18:1-, C20:1- and C20:4-ceramides, dihydroceramides and phytoceramides into sphingoid bases like sphingosine and free fatty acids at alkaline pH (PubMed:20068046, PubMed:26792856, PubMed:20207939, PubMed:11356846, PubMed:30575723). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:20068046). Controls the generation of sphingosine in erythrocytes, and thereby sphingosine-1-phosphate in plasma (PubMed:20207939). Through the regulation of ceramides and sphingosine-1-phosphate homeostasis in the brain may play a role in neurons survival and function (By similarity). By regulating the levels of proinflammatory ceramides in immune cells and tissues, may modulate the inflammatory response (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.