SLC29A3 (NM_018344) Human Untagged Clone

SKU SC113561

SLC29A3 (untagged)-Human solute carrier family 29 (nucleoside transporters), member 3 (SLC29A3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: SLC29A3
• Synonyms: ENT3; HCLAP; HJCD; PHID
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113561 sequence for NM_018344 edited (data generated by NextGen Sequencing); ATGGCCGTTGTCTCAGAGGACGACTTTCAGCACAGTTCAAACTCCACCTACAGAACCACA; AGCAGCAGTCTCCGAGCTGACCAGGAGGCACTGCTTGAGAAGCTGCTGGACCGCCCGCCC; CCTGGCCTGCAGAGGCCCGAGGACCGCTTCTGTGGCACATACATCATCTTCTTCAGCCTG; GGCATTGGCAGTCTACTGCCATGGAACTTCTTTATCACTGCCAAGGAGTACTGGATGTTC; AAACTCCGCAACTCCTCCAGCCCAGCCACCGGGGAGGACCCTGAGGGCTCAGACATCCTG; AACTACTTTGAGAGCTACCTTGCCGTTGCCTCCACCGTGCCCTCCATGCTGTGCCTGGTG; GCCAACTTCCTGCTTGTCAACAGGGTTGCAGTCCACATCCGTGTCCTGGCCTCACTGACG; GTCATCCTGGCCATCTTCATGGTGATAACTGCACTGGTGAAGGTGGACACTTTCTCCTGG; ACCCGTGGCTTTTTTGCGGTCACCATTGTCTGCATGGTGATCCTCAGCGGTGCCTCCACT; GTCTTCAGCAGCAGCATCTACGGCATGACCGGCTCCTTTCCTATGAGGAACTCCCAGGCA; CTGATATCAGGAGGAGCCATGGGCGGGACGGTCAGCGCCGTGGCCTCATTGGTGGACTTG; GCTGCATCCAGTGATGTGAGGAACAGCGCCCTGGCCTTCTTCCTGACGGCCACCATCTTC; CTCGTGCTCTGCATGGGACTCTACCTGCTGCTGTCCAGGCTGGAGTATGCCAGGTACTAC; ATGAGGCCTGTTCTTGCGGCCCATGTGTTTTCTGGTGAAGAGGAGCTTCCCCAGGACTCC; CTCAGTGCCCCTTCGGTGGCCTCCAGATTCATTGATTCCCACACACCCCCTCTCCGCCCC; ATCCTGAAGAAGACGGCCAGCCTGGGCTTCTGTGTCACCTACGTCTTCTTCATCACCAGC; CTCATCTACCCCGCCGTCTGCACCAACATCGAGTCCCTCAACAAGGGCTCGGGCTCACTG; TGGACCACCAAGTTTTTCATCCCCCTCACTACCTTCCTCCTGTACAACTTTGCTGACCTA; TGTGGCCGGCAGCTCACCGCCTGGATCCAGGTGCCAGGGCCCAATAGCAAGGCGCTCCCA; GGGTTCGTGCTCCTCCGGACCTGCCTCATCCCCCTCTTCGTGCTCTGTAACTACCAGCCC; CGCGTCCACCTGAAGACTGTGGTCTTCCAGTCCGATGTGTACCCCGCACTCCTCAGCTCC; CTGCTGGGGCTCAGCAACGGCTACCTCAGCACCCTGGCCCTCCTCTACGGGCCTAAGATT; GTGCCCAGGGAGCTGGCTGAGGCCACGGGAGTGGTGATGTCCTTTTATGTGTGCTTGGGC; TTAACACTGGGCTCAGCCTGCTCTACCCTCCTGGTGCACCTCATCTAG; ; Clone variation with respect to NM_018344.5; 473 c=>t;714 t=>c;715 g=>a;976 a=>g;1008 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018344 unedited; NGGTCGCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGACATGGC; CGTTGTCTCAGAGGACGACTTTCAGCACAGTTCAAACTCCACCTACAGAACCACAAGCAG; CAGTCTCCGAGCTGACCAGGAGGCACTGCTTGAGAAGCTGCTGGACCGCCCGCCCCCTGG; CCTGCAGAGGCCCGAGGACCGCTTCTGTGGCACATACATCATCTTCTTCAGCCTGGGCAT; TGGCAGTCTACTGCCATGGAACTTCTTTATCACTGCCAAGGAGTACTGGATGTTCAAACT; CCGCAACTCCTCCAGCCCAGCCACCGGGGAGGACCCTGAGGGCTCAGACATCCTGAACTA; CTTTGAGAGCTACCTTGCCGTTGCCTCCACCGTGCCCTCCATGCTGTGCCTGGTGGCCAA; CTTCCTGCTTGTCAACAGGGTTGCAGTCCACATCCGTGTCCTGGCCTCACTGACGGTCAT; CCTGGCCATCTTCATGGTGATAACTGCACTGGTGAAGGTGGACACTTTCTCCTGGACCCG; TGGCTTTTTTGCGGTCACCATTGTCTGCATGGTGATCCTCAGCGGTGCCTCCACTGTCTT; CAGCAGCAGCATCTACGGCATGACCGGCTCCTTTCCTATGAGGAACTCCCAGGCACTGAT; ATCAGGAGGAGCCATGGGCGGGACGGTCAGCGCCGTGGCCTCATTGGTGGACTTGGCTGC; ATCCAGTGATGTGAGGAACAGCGCCCTGGCCTTCTTCCTGACGGCCACCATCTTCCTCGT; GCTCTGCATGGGACTCTACCTGCTGCTGTCCAGGCTGGAGTATGCCANGTACTACATGAN; GCCTNGTCTTTGCGCCATGTGTTNTCTGGTGAAGAGGAGCTTCCCAGGACTNCCTCAGTG; CCCCTTTCGTGGCCCTCAGATTCATGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018344 unedited; NNNNTTCGNCNAGNTNATTTNATTNACGNCNCCTTNGNNAACCAATGGNNCTTTGNACAT; NNGTTGTAAACAGGNAACACTNGAAATACCCTCATCTTGACCCAGGCCATAGACCACCAG; GTCATGAGGATCCCTCTTTTTTTGAAAACCCAGGCCAGACATGCTTGATCCCTTGCACAG; GGAGGCCCTCTGGTCCCTCTGGAATGAAGCATTCATGGAAGGCCCATCTTTCTGGGTTTT; CCAGTCTGGCTAGTGGGCAGTTGGGGGGGGGCAGAACACAGGGGTTCTGTGTCCCACTTG; GGTTTTGGGTCCTGAAGACTGACCATTCCCTCCGGACTNCNACTCCCACTGGAAACTCCT; TGTAGGTAANGATTTTTTTTNNTTTTATTTCCTTCTCCCTCACACCCCCACTTCATCGCC; CACNCTCTCTAGTCTCTACACGTTCCTTACTTTAATTCCCCCTCCGTTTCCCTCCCCATT; CCCCTCCCACCTTACCTTTTCCTCTTTTTCCTCCTGTCTTATACTTNTTCTCCCTATCCT; ACTCTCTCTCATCCCCCTTTTCCTCTAATTTATTTTCTTTTTTCTTTTTTATNTTCTCCC; CTTTTCTTTCTTTTCCGTTCCGCCTCTCATCTCCCCACCCATTCTNTCCTCTCTTTATTT; GATTTCCCCTCCCCTTCTCTCCGTCTTTTATTTTACCTCTACTCCCCCTCGTTTCCCGAT; CTCTTCCTCTCATTTTTATCCTCTCCCCTCCTTTTTCTTATTCCTTTTATCCTACATCCG; CCCCCCTTTCTACAGCATTATACACTCCGCACGTCCGCCCTCCACATTTATTTTGCGCGC; ATTAGTCCAGCGCCCCTCTCCTGTTCATGCCTTTAGATATTCTCTTCGTCTACTAATTTC; ACTATATTTTACTACTGTCTGTCTCCTCTCCCCTCCTCCCTATCTCCTCTCCTCCTCATC; ATAGCTCATACTGTTTCTGTTATCACTTATTTATATTCTCATTATTCATACGTTCTTCCT; TTTATACCCTTCTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_018344
• Insert Size: 2260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018344.3, NP_060814.3
• RefSeq Size: 2243 bp
• RefSeq ORF: 1428 bp
• Locus ID: 55315
• UniProt ID: Q9BZD2
• Cytogenetics: 10q22.1
• Domains: Nucleoside_tran
• Protein Families: Transmembrane
• Summary: This gene encodes a nucleoside transporter. The encoded protein plays a role in cellular uptake of nucleosides, nucleobases, and their related analogs. Mutations in this gene have been associated with H syndrome, which is characterized by cutaneous hyperpigmentation and hypertrichosis, hepatosplenomegaly, heart anomalies, and hypogonadism. A related disorder, PHID (pigmented hypertrichosis with insulin-dependent diabetes mellitus), has also been associated with mutations at this locus. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2010]; Transcript Variant: This variant (1) encodes the longer isoform (a).