GALNT1 (NM_020474) Human Untagged Clone

SKU SC113088

GALNT1 (untagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) (GALNT1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GALNT1
• Synonyms: GALNAC-T1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113088 sequence for NM_020474 edited (data generated by NextGen Sequencing); ATGAGAAAATTTGCATACTGCAAGGTGGTCCTAGCCACCTCCTTGATTTGGGTACTCTTG; GATATGTTCCTGCTGCTTTACTTCAGTGAATGCAACAAATGTGATGAAAAAAAGGAGAGA; GGACTTCCTGCTGGAGATGTTCTAGAGCCAGTACAAAAGCCTCATGAAGGTCCTGGAGAA; ATGGGGAAACCAGTCGTCATTCCTAAAGAGGATCAAGAAAAGATGAAAGAGATGTTTAAA; ATCAATCAGTTCAATTTAATGGCAAGTGAGATGATTGCACTCAACAGATCTTTACCAGAT; GTTAGGTTAGAAGGGTGTAAAACAAAGGTGTATCCAGATAATCTTCCTACAACAAGTGTG; GTGATTGTTTTCCACAATGAGGCTTGGAGCACACTTCTGCGAACTGTCCATAGTGTCATT; AATCGCTCACCAAGACACATGATAGAAGAAATTGTTCTAGTAGATGATGCCAGTGAAAGA; GACTTTTTGAAAAGGCCTTTAGAGAGTTATGTGAAAAAACTAAAAGTACCAGTTCATGTA; ATTCGAATGGAACAACGTTCTGGATTGATCAGAGCTAGATTAAAAGGAGCTGCTGTGTCT; AAAGGCCAAGTGATCACCTTCCTGGATGCCCATTGTGAGTGTACAGTGGGATGGCTGGAG; CCTCTCTTGGCCAGGATCAAACATGACAGGAGAACAGTGGTGTGTCCCATCATCGATGTG; ATCAGTGATGATACTTTTGAGTACATGGCAGGCTCTGATATGACCTATGGTGGGTTCAAC; TGGAAGCTCAATTTTCGCTGGTATCCTGTTCCCCAAAGAGAAATGGACAGAAGGAAAGGT; GATCGGACTCTTCCTGTCAGGACACCTACCATGGCAGGAGGCCTTTTTTCAATAGACAGA; GATTACTTTCAGGAAATTGGAACATATGATGCTGGAATGGATATTTGGGGAGGAGAAAAC; CTAGAAATTTCCTTTAGGATTTGGCAGTGTGGAGGAACTTTGGAAATTGTTACATGCTCA; CATGTTGGACATGTGTTTCGGAAAGCTACACCTTACACGTTTCCAGGAGGCACAGGGCAG; ATTATCAATAAAAATAACAGACGACTTGCAGAAGTGTGGATGGATGAATTCAAGAATTTC; TTCTATATAATTTCTCCAGGTGTTACAAAGGTAGATTATGGAGATATATCGTCAAGAGTT; GGTCTAAGACACAAACTACAATGCAAACCTTTTTCCTGGTACCTAGAGAATATATATCCT; GATTCTCAAATTCCACGTCACTATTTCTCATTGGGAGAGATACGAAATGTGGAAACGAAT; CAGTGTCTAGATAACATGGCTAGAAAAGAGAATGAAAAAGTTGGAATTTTTAATTGCCAT; GGTATGGGGGGTAATCAGGTTTTCTCTTATACTGCCAACAAAGAAATTAGAACAGATGAC; CTTTGCTTGGATGTTTCCAAACTTAATGGCCCAGTTACAATGCTCAAATGCCACCACCTA; AAAGGCAACCAACTCTGGGAGTATGACCCAGTGAAATTAACCCTGCAGCATGTGAACAGT; AATCAGTGCCTGGATAAAGCCACAGAAGAGGATAGCCAGGTGCCCAGCATTAGAGACTGC; AATGGAAGTCGGTCCCAGCAGTGGCTTCTTCGAAACGTCACCCTGCCAGAAATATTCTGA; ; Clone variation with respect to NM_020474.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020474 unedited; TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGGGTATGAACGTGA; TTTCTGATGAAACTGGATTGGAATAATTTTCATGATCTTTGTATATTTATATATATATAT; TTTTAAATTTTGCATTTGACTTAAAGTGCCATGAGAAAATTTGCATACTGCAAGGTGGTC; CTAGCCACCTCCTTGATTTGGGTACTCTTGGATATGTTCCTGCTGCTTTACTTCAGTGAA; TGCAACAAATGTGATGAAAAAAAGGAGAGAGGACTTCCTGCTGGAGATGTTCTAGAGCCA; GTACAAAAGCCTCATGAAGGTCCTGGAGAAATGGGGAAACCAGTCGTCATTCCTAAAGAG; GATCAAGAAAAGATGAAAGAGATGTTTAAAATCAATCAGTTCAATTTAATGGCAAGTGAG; ATGATTGCACTCAACAGATCTTTACCAGATGTTAGGTTAGAAGGGTGTAAAACAAAGGTG; TATCCAGATAATCTTCCTACAACAAGTGTGGTGATTGTTTTCCACAATGAGGCTTGGAGC; ACACTTCTGCGAACTGTCCATAGTGTCATTAATCGCTCACCAAGACACATGATAGAAGAA; ATTGTTCTAGTAGATGATGCCAGTGAAAGAGACTTTNTGAAAAGGCCTTTAGAGAGTTAT; GTGAAAAACTAANAGTACCAGTTCATGTAATTCGAATGGAACAACGTTCTGGATTGATCA; GAGCTAGATTAAAGGAGCTGCTGTGTCTAAAGGCAAGTGATCACCTTCCTGGATGCCCAT; TGTGAGTGTACAGTGGGATGGCTGGAGCCTCTCTTGGNCAGATCAACATGACAGAGAACA; GNGGTGTGTCCATCATCGATGTGATCAGTGATGATACTTTTGATACATGGCAGCTCTGAT; ATGACTTATGGTGGGGTCAACTGGAGCTCATTTTGCTTGTTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020474 unedited; CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTATTCAGGAATATTTTGT; ATTTTCAAAGTATTTAGGAATTCTTTAATTAGAAAAATAAAGGCAATGGGATTATCTATC; AGGTTCTAAAAGTACCCATGAATTAAATCACTTACATCTCCCATTTAAAACAAAACCTTA; AATCACATTGCTGTTAAGCTTTCATACAGTAAGAAATAAGACCAAGCTATGCTGGAGTAC; AAAAACCTGAAATTCTGAGCATGAAAATAATTTATTGATTAGACATCTTGCTTATTCAAG; CATTAGGCCTACTGGAGATAGAATTAACACAGCTCATGGAAAAGGAAAAAAATTCCATTC; AGCACCTGCTTCCTTTCAAATTTCCTGGGGACATGGTTTATGCATTTTGTACTATAGACT; CACCCTAAAGCATCCCCATGTGCTTGATGTTGATTAACTTGAACAGGATATGAGAAACTT; TGTGTACACTCAACTTGGCAAATATCATTAGCGATGCGACTTTCGGAAACATTTTTAGGC; ACAAGAAATACCACTGCCTGCAGTGAAAGCCCTTGATATAAAACAAAAAACCCCATTAAA; TTCACTTCTCCAATCTGACAGAAAATTTTTGCTTTTTTGCCAACAAACTGTTCTACCTAT; AAGAAAAAGCGCTAATGATATTCCTCCTCCCAAATAAAAGGTACCTCTTAATGAACACTT; TACTTGACCCTTAGGAGACTTGTGGGCTCAAACACATCCGAGACACATAACCTGTTTATC; CTAAATATATTCAGGAAAAAATTCAGCCCCTAATATGGATAACATCCGCCCTTCTTGGTA; AGCCCTCCAAACCCCCCTCACACTTTTCCCCCTCTCTTTTCCAACACCCGCGAACACTTC; ACCCTTCCCCCAATCCCAAACTCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_020474
• Insert Size: 3780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020474.2, NP_065207.2
• RefSeq Size: 3778 bp
• RefSeq ORF: 1680 bp
• Locus ID: 2589
• UniProt ID: Q10472
• Cytogenetics: 18q12.2
• Domains: Glycos_transf_2, RICIN
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• Summary: This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. Transcript variants derived from this gene that utilize alternative polyA signals have been described in the literature. [provided by RefSeq, Jul 2008]