AICDA (NM_020661) Human Untagged Clone

CAT#: SC113038

AICDA (untagged)-Human activation-induced cytidine deaminase (AICDA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AICDA
• Synonyms: AID; ARP2; CDA2; HEL-S-284; HIGM2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113038 sequence for NM_020661 edited (data generated by NextGen Sequencing)
  ATGGACAGCCTCTTGATGAACCGGAGGAAGTTTCTTTACCAATTCAAAAATGTCCGCTGG
  GCTAAGGGTCGGCGTGAGACCTACCTGTGCTACGTAGTGAAGAGGCGTGACAGTGCTACA
  TCCTTTTCACTGGACTTTGGTTATCTTCGCAATAAGAACGGCTGCCACGTGGAATTGCTC
  TTCCTCCGCTACATCTCGGACTGGGACCTAGACCCTGGCCGCTGCTACCGCGTCACCTGG
  TTCACCTCCTGGAGCCCCTGCTACGACTGTGCCCGACATGTGGCCGACTTTCTGCGAGGG
  AACCCCAACCTCAGTCTGAGGATCTTCACCGCGCGCCTCTACTTCTGTGAGGACCGCAAG
  GCTGAGCCCGAGGGGCTGCGGCGGCTGCACCGCGCCGGGGTGCAAATAGCCATCATGACC
  TTCAAAGATTATTTTTACTGCTGGAATACTTTTGTAGAAAACCATGAAAGAACTTTCAAA
  GCCTGGGAAGGGCTGCATGAAAATTCAGTTCGTCTCTCCAGACAGCTTCGGCGCATCCTT
  TTGCCCCTGTATGAGGTTGATGACTTACGAGACGCATTTCGTACTTTGGGACTTTGA
  
  Clone variation with respect to NM_020661.2
  465 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020661 unedited
  ACGACTCACTATATGGCGGCCGCGAATTCGGCACGAGGGAACCATCATTAATTGAAGTGA
  GATTTTTCTGGCCTGAGACTTGCAGGGAGGCAAGAAGACACTCTGGACACCACTATGGAC
  AGCCTCTTGATGAACCGGAGGAAGTTTCTTTACCAATTCAAAAATGTCCGCTGGGCTAAG
  GGTCGGCGTGAGACCTACCTGTGCTACGTAGTGAAGAGGCGTGACAGTGCTACATCCTTT
  TCACTGGACTTTGGTTATCTTCGCAATAAGAACGGCTGCCACGTGGAATTGCTCTTCCTC
  CGCTACATCTCGGACTGGGACCTAGACCCTGGCCGCTGCTACCGCGTCACCTGGTTCACC
  TCCTGGAGCCCCTGCTACGACTGTGCCCGACATGTGGCCGACTTTCTGCGAGGGAACCCC
  AACCTCAGTCTGAGGATCTTCACCGCGCGCCTCTACTTCTGTGAGGACCGCAAGGCTGAG
  CCCGAGGGGCTGCGGCGGCTGCACCGCGCCGGGGTGCAAATAGCCATCATGACCTTCAAA
  GATTATTTTTACTGCTGGAATACTTTTGTAGAAAACCATGAAAGAACTTTCAAAGCCTGG
  GAAGGGCTGCATGAAAATTCAGTTCGTCTCTCCAGACAGCTTCNGCGCATCCTTTTGCCC
  CTGTATGAGGTTGATGACTTACGAGACGCATTTCGTACTTTGGGACTTTGATAGCAACTT
  CCAGGAATGTCACACACGATGAAATTN
  
• Restriction Sites: Please inquire
• ACCN: NM_020661
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020661.1, NP_065712.1
• RefSeq Size: 2791 bp
• RefSeq ORF: 597 bp
• Locus ID: 57379
• UniProt ID: Q9GZX7
• Cytogenetics: 12p13.31
• Protein Families: Druggable Genome
• Protein Pathways: Primary immunodeficiency
• Gene Summary: This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. AICDA is specifically expressed and active in germinal center-like B cells. In the germinal center, AICDA is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. An epigenetic role in neoplastic transformation and lymphoma progression has been experimentally ascribed to AICDA using mouse models. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq, Jul 2020]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).