POLR2L (NM_021128) Human Untagged Clone

SKU SC112974

POLR2L (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide L, 7.6kDa (POLR2L)

Product Data

• Type: Human Untagged Clone
• Target Symbol: POLR2L
• Synonyms: hRPB7.6; RBP10; RPABC5; RPB7.6; RPB10; RPB10beta
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021128, the custom clone sequence may differ by one or more nucleotides; ; ; ATGATCATCCCTGTACGCTGCTTCACTTGTGGCAAGATCGTCGGCAACAAGTGGGAGGCTTACCTGGGGC; TGCTGCAGGCCGAGTACACCGAGGGGGATGCGCTGGATGCCCTGGGCCTGAAGCGCTACTGCTGCCGCCG; GATGCTGCTGGCCCACGTGGACCTGATCGAGAAGCTGCTCAATTATGCACCCCTGGAGAAGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021128 unedited; CCTTTTCGGATTNTGTAAACGATTCACTATAGGCGGCCGCGCAATCGGCACGAGGCGCCG; CCGCCATGATCATCCCTGTACGCTGCTTCACTTGTGGCAAGATCGTCGGCAACAAGTGGG; AGGCTTACCTGGGGCTGCTGCAGGCCGAGTACACCGAGGGGGACGCGCTGGATGCCCTGG; GCCTGAAGCGCTACTGCTGCCGCCGGATGCTGCTGGCCCACGTGGACCTGATCGAGAAGC; TGCTCAATTATGCACCCCTGGAGAAGTGACCACGCTGGAACCCACCCACCCGCTGTGCTG; ACCATGGGCCCTGAGCGTCCTACCCCGAATTCACGAGGCTGAGGCATCCGGGAGCTGGCG; TAATGCCTGGCCGCAGTGTGTGTGTATCCCATACCCCACTCTGGAAGGAACCATCCAGTA; AAGGTCTTTCAGAACCAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC; GGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCC; TCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGCCCTAATAAAATTAAG; CTGCATCATTTTGTCTGACTAGGCGCCCTTCTATAATATTATGGGGCGGAGGGGGGTGGC; TATGGACCCATGGGGCCCAGTTGCCAAACACACACCTGTAAGGCCCCGCCGGGGCCCAAC; TGCCGAAACCAGCCTGGACCGGCCCGGGCCACAAATCTTCGGCTCACTTGGCCATCTTTC; CCCTTCCTGGGTTCCAACCGAATCTCCCCGCCCCCAGCCTTCCCGAGGTGGTCGGGGAAT; ACCAGCAACTGCCTTGACCCACCCTCAACATCACCTCCTGCTTCTTTGGCCAAACCACCC; GGCTTCCCCACCCTTGCCACCCCCGCCCCACCAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021128 unedited; CAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTTTTTGGGTCTGAAAGACTTTACTGGAT; GGTTCCTTCCAGAGTGGGGTATGGGATACACACACACTGCGGCCAGGCATTACGCCAGCT; CCCGGATGCCTCAGCCTCGTGAATTCGGGGTAGGACGCTCAGGGCCCATGGTCAGCACAG; CGGGTGGGTGGGTTCCAGCGTGGTCACTTCTCCAGGGGTGCATAATTGAGCAGCTTCTCG; ATCAGGTCCACGTGGGCCAGCAGCATCCGGCGGCAGCAGTAGCGCTTCAGGCCCAGGGCA; TCCAGCGCGTCCCCCTCGGTGTACTCGGCCTGCAGCAGCCCCAGGTAAGCCTCCCACTTG; TTGCCGACGATCTTGCCACAAGTGAAGCAGCGTACAGGGATGATCATGGCGGCGGCGCCT; CGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTA; AACGAGCTCTGCTTATATAGACCTNCCACCGTACACGCCTACCGNCCATTTGCGTCAACG; GGGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGGTGCAAAACAAACTCC; CATTGACGTCAATGGGGTGGAGACTTGGGAAATCCCGTGAGTCAAACCGCTATCCCGCCC; ATTGGTGTACTGNCAAANCCGCATCACATGGTAATAGCGATGACTAATACGTANATGTAC; TGCCAAGTANGAAAGTCCCGTAAGTCATGTACTGGGCATAATGCCCAGCGGGCCATTTAC; CGGTATTGACGTCAATAGGGGGCCGAACTTTGCATATGAATCACTTGATGTACTCGCCAG; TGGGCAGTTTACCGCAANTCCTCCACCCATTGCCGTCAATGGAAAGCCCCATTGTCCGTA; CCTATGGAACATACGGTCTTTTTGACNTCAATGGCGCGGGGTCTNTTGGNCGGCAACCCC; CCCGGCCCTTTACCGCATTATATTAACCCGGATTCCTCTTGGGCTTGAACCAAGACCCGA; T;
• Restriction Sites: NotI-NotI
• ACCN: NM_021128
• Insert Size: 400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021128.3, NP_066951.1
• RefSeq Size: 927 bp
• RefSeq ORF: 204 bp
• Locus ID: 5441
• UniProt ID: P62875
• Cytogenetics: 11p15.5
• Domains: RNA_pol_N
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Summary: This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains four conserved cysteines characteristic of an atypical zinc-binding domain. Like its counterpart in yeast, this subunit may be shared by the other two DNA-directed RNA polymerases. [provided by RefSeq, Jul 2008]