G protein beta 4 (GNB4) (NM_021629) Human Untagged Clone

SKU SC112904

GNB4 (untagged)-Human guanine nucleotide binding protein (G protein), beta polypeptide 4 (GNB4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: G protein beta 4
• Synonyms: CMTD1F
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112904 sequence for NM_021629 edited (data generated by NextGen Sequencing); ATGAGCGAACTGGAACAGTTGAGGCAAGAAGCAGAACAACTGCGGAATCAGATTCAGGAT; GCTCGGAAAGCATGTAATGATGCAACGCTTGTTCAGATTACATCAAATATGGACTCCGTG; GGTCGAATACAAATGCGAACAAGACGTACACTGAGGGGCCACCTAGCTAAAATCTATGCT; ATGCATTGGGGATACGATTCCAGGCTGCTAGTCAGTGCTTCTCAAGATGGAAAATTAATT; ATTTGGGATAGCTATACAACAAATAAGATGCATGCTATTCCTTTGAGGTCCTCCTGGGTG; ATGACCTGTGCTTATGCTCCCTCTGGTAATTATGTTGCCTGTGGAGGCTTGGACAACATC; TGCTCTATATATAACTTAAAGACCAGAGAGGGAAATGTGAGAGTAAGCCGAGAGTTGCCA; GGTCACACAGGGTACTTGTCCTGCTGTCGTTTTTTAGATGACAGCCAAATTGTTACAAGT; TCAGGAGATACAACTTGTGCTTTATGGGACATCGAAACTGCCCAGCAGACCACCACATTC; ACTGGGCATTCTGGAGATGTGATGAGTCTTTCTTTGAGTCCTGACATGAGGACTTTTGTT; TCTGGTGCTTGTGATGCCTCTTCCAAATTATGGGATATTCGAGATGGAATGTGTAGACAG; TCTTTCACGGGACATGTCTCAGATATCAATGCTGTCAGTTTTTTCCCAAATGGATATGCC; TTCGCCACTGGCTCTGATGATGCCACTTGCCGGCTCTTTGACCTTCGTGCAGATCAAGAG; TTATTATTGTATTCTCATGACAATATCATCTGTGGAATCACTTCTGTAGCCTTCTCAAAA; AGTGGGCGTCTCTTGTTGGCTGGTTACGATGACTTTAATTGTAATGTATGGGACACGCTA; AAAGGAGATCGTGCAGGTGTCCTTGCTGGTCATGACAACCGTGTGAGCTGCTTAGGTGTA; ACTGATGATGGCATGGCTGTGGCAACAGGCTCTTGGGACAGTTTTCTTAGAATCTGGAAT; TAA; ; Clone variation with respect to NM_021629.3; 117 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021629 unedited; TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCACGCCGGCTCCGCGCT; CCGGGGCTGCTGAGCGCCCAGCGGACACCGGCAGCGCGCGGTCGACGCGGGCCTGAGCTC; CCTCCAGCTGTTTTCACTCATTAGCTCCTGAGGTAAACAAATTGAAAAAATGAGCGAACT; GGAACAGTTGAGGCAAGAAGCAGAACAACTGCGGAATCAGATTCAGGATGCTCGGAAAGC; ATGTAATGATGCAACGCTTGTTCAGATTACATCAAATATGGACTCCGTGGGTCGAATACA; AATGCGAACAAGACGTACACTGAGGGGCCACCTAGCTAAAATCTATGCTATGCATTGGGG; ATACGATTCCAGGCTGCTAGTCAGTGCTTCTCAAGATGGAAAATTAATTATTTGGGATAG; CTATACAACAAATAAGATGCATGCTATTCCTTTGAGGTCCTCCTGGGTGATGACCTGTGC; TTATGCTCCCTCTGGTAATTATGTTGCCTGTGGAGGCTTGGACAACATCTGCTCTATATA; TAACTTAAAGACCAGAGAGGGAAATGTGAGAGTAAGCCGAGAGTTGCCAGGTCACACAGG; GTACTTGTCCTGCTGTCGTTTTTTAGATGACAGCCAAATTGTTACAAGTTCAGGAGATAC; AACTTGTGCTTTATGGGACATCGAAACTGCCCAGCAGACCACCACATTCACTGGGCATTC; TGGAGATGTGATGAGTCTTTCTTTGAGTCCTGACATGAAGACTTTGTTTCTGGTGCTTGT; GATGCCCTCTTCCAATATGGGATATTCGAGATGGATGTGTAGACAGTCTTTCACGGNACA; TGTCTCAGAATCATGCTGTAGTTTTTTCCAAATGGATATGCTTCGCACTGGTCTGAGATG; CACTTGCGCTCTTTGACTTGTGCAGATCAGAGTATATTGAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021629 unedited; GCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCATTTGAGATGGAGTC; TCACTCTGTCGCCTAGGCTGGAGTGCAGTGGCACAATCTCGGCTCACTGCAACCTCTGCC; TCCCGGGTTCAGGCGAATCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGAATGT; GCCACCACGCCCGGCTAATGTTTGTATTTTTAGAAGAGATGGGGTTTCACCATGTTGGGT; AGGCTGGTCGCGAACTCCTGACCTCAAGTGATCTGCCCACCTTGGCCTCCCAAATTGCTG; GGATTATAGGTGTAAGCCACTCTGCCCAGCCAAAAAGTTAATAGAAATCTTAATCAAAAT; ATAAATATACATTCATAACTGCCATCTACAATATTCTGTTTTGGTCCTATATCTTAATTT; GCCTTTATCCACTTTATTATTCTAATTGTCAACTGATTTTAATAAAACTGATACATCTTT; ACAGTTGATGCACATATTAGCACATTCACAACATAAAAACAAAAATTTAGAGGTAAAGTT; TGGGAGTATTTAGGTTAAGTTACACATGTTCAAAAGTTAACACAGACCTATCTGAAAAGT; AACTAAGTTACAAAACAATGCACGTCTATATATCAGAATAATCCANGACTTTACCATCAA; GNCAGACTAACAATCTTCTATTATCAGTCATTTAGACTTATTTCTAAAGAGAATCCACAG; GAAAGTATCTTTAACTTGGTCGTGGGGGACTTCTANTAAGAGAACATATTATACCACAGA; AAACTGTTTATATAGCTCTCCTACNCTTTAAGACTGCTTTANGATTTTTTATTTTGCTTT; TAGGGGATCACTTAATCTAGAACTCTTTGAGCAAACACTTAAAACAATTTTTGTGCTAAG; GAGGAAGAATACTCCAAAGTGCCAAAGAACAAAGCTTAAGTTAAANTTAAGGCCCGATTT; CCTCATTCAATTTTAGTGCCACTTAAAACCCCGGT;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_021629
• Insert Size: 3220 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021629.2, NP_067642.1
• RefSeq Size: 3302 bp
• RefSeq ORF: 1023 bp
• Locus ID: 59345
• UniProt ID: Q9HAV0
• Cytogenetics: 3q26.33
• Domains: WD40
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway
• Summary: Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. [provided by RefSeq, Jul 2008]