CD40L (CD40LG) (NM_000074) Human Untagged Clone

SKU SC112886

CD40LG (untagged)-Human CD40 ligand (CD40LG)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CD40L
• Synonyms: CD40L; CD154; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000074 edited; ATGATCGAAACATACAACCAAACTTCTCCCCGATCTGCGGCCACTGGACTGCCCATCAGC; ATGAAAATTTTTATGTATTTACTTACTGTTTTTCTTATCACCCAGATGATTGGGTCAGCA; CTTTTTGCTGTGTATCTTCATAGAAGGTTGGACAAGATAGAAGATGAAAGGAATCTTCAT; GAAGATTTTGTATTCATGAAAATGATACAGAGATGCAACACAGGAGAAAGATCCTTATCC; TTACTGAACTGTGAGGAGATTAAAAGCCAGTTTGAAGGCTTTGTGAAGGATATAATGTTA; AACAAAGAGGAGACGAAGAAAGAAAACAGCTTTGAAATGCAAAAAGGTGATCAGAATCCT; CAAATTGCGGCACATGTCATAAGTGAGGCCAGCAGTAAAACAACATCTGTGTTACAGTGG; GCTGAAAAAGGATACTACACCATGAGCAACAACTTGGTAACCCTGGAAAATGGGAAACAG; CTGACCGTTAAAAGACAAGGACTCTATTATATCTATGCCCAAGTCACCTTCTGTTCCAAT; CGGGAAGCTTCGAGTCAAGCTCCATTTATAGCCAGCCTCTGCCTAAAGTCCCCCGGTAGA; TTCGAGAGAATCTTACTCAGAGCTGCAAATACCCACAGTTCCGCCAAACCTTGCGGGCAA; CAATCCATTCACTTGGGAGGAGTATTTGAATTGCAACCAGGTGCTTCGGTGTTTGTCAAT; GTGACTGATCCAAGCCAAGTGAGCCATGGCACTGGCTTCACGTCCTTTGGCTTACTCAAA; CTCTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000074 unedited; NGGGTGCGCATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGTAACGTT; TTTGCTGGGAGAGAAGACTACGAAGCACATTTTCCAGGAAGTGTGGGCTGCAACGATTGT; GCGCTCTTAACTAATCCTGAGTAAGGTGGCCACTTTGACAGTCTTCTCATGCTGCCTCTG; CCACCTTCTCTGCCAGAAGATACCATTTCAACTTTAACACAGCATGATCGAAACATACAA; CCAAACTTCTCCCCGATCTGCGGCCACTGGACTGCCCATCAGCATGAAAATTTTTATGTA; TTTACTTACTGTTTTTCTTATCACCCAGATGATTGGGTCAGCACTTTTTGCTGTGTATCT; TCATAGAAGGTTGGACAAGATAGAAGATGAAAGGAATCTTCATGAAGATTTTGTATTCAT; GAAAATGATACAGAGATGCAACACAGGAGAAAGATCCTTATCCTTACTGAACTGTGAGGA; GATTAAAAGCCAGTTTGAAGGCTTTGTGAAGGATATAATGTTAAACAAAGAGGAGACGAA; GAAAGAAAACAGCTTTGAAATGCAAAAAGGTGATCAGAATCCTCAAATTGCGGCACATGT; CATAAGTGAGGCCAGCAGTAAAACAACATCTGTGTTACAGTGGGCTGAAAAAGGATACTA; CACCATGAGCAACAACTTGGTAACCCTGGAAAATGGGAAACAGCTGACCGTTAANAGACA; GGGACTCTATTATATCTATGCCCAAGTCACCTTCTGTTCCAAATCGGAAGCTTCGAGTCA; AGCTCCATTTATAGCCAGCCTCTGCCTAAAGTCCCCCGGTAGATTCGAGAGAAATCTACT; CAGAGCTGCAAATACCCACAGTTNCGCCAAACCTTTGCGGNNCACATNCATTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000074 unedited; CGGCACGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTAATGTTAAAAGGTCTTTA; TTATTCAAAAAATAAATAACAAGATAATTACAAGTTCACTCTGGAAACAATGGAGACTGC; AGGTAGATACAATAGATTTCCTTTAAATACAGTCATTTCGTAATGAGGAGTGGGCAGGCT; CAGGGCTGCACTCCCTACTCCTCACCCTCATCTATAGTGGTAGAGATAGGGACAGGGTGG; AAAGAAGAGAACTGACTAGCAACGGCCTGACTCTGTGTGTGTGTGTGTGTGTGTGTGTGT; GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAAAGGGGGATTGAGAAGAGAGACTGGG; GGAGGGAAGAGACTGACAAAGGGAAACAGAGAGATTGAGAAAGAGAGATTGGAAGAAAGA; GGTTGAGAGAGAGAGACTGAAAGAGAGAGATGGAGAGAGAGAGATTGAAAGAGAATGAAT; AACTGTCCGAGAGGGTGGCGGGGGGGGGGGGGTTTCGGTTCTCCACTGTGTTAGACGTTC; TAGAGCCTGGCCCCCTCCAACAAGGCCATAGGAACCCAGAGTCAACCATAACTGAATGTG; AGCTGAGGCTCCCCATTTCCCTTCTGCATCTTCACTGATAAATCAAATGCATTATCCATG; GAAAGACACTGCACGCAAAGAAACAAAACTGAACTTGGCAAGTTATCTGCTGTTTACTCA; GAATTCAACCTGTGCATAATGGCCCTGTCTTTTCATAGGACTCTCTGGAAGTCTGCATCA; GAGGGGTTGCTGCTTCAAAATATAACCCTTTAGGAGCAAGGCCCGTTTTGCTTCATGTGA; CCTGTAATCCATTTATTACGGTTCACATGCCCTGGAAAGGATAATAAATAGTCCTCCATA; AGGAGGACCCAGGGTTATAAAAAGCAGTTAACGGGGGGGGGCCTAACCCTCGGGCTCGTT; TAAAGAAACCCCACGCCAACTCTCACCCAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_000074
• Insert Size: 786 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000074.2, NP_000065.1
• RefSeq Size: 1834 bp
• RefSeq ORF: 786 bp
• Locus ID: 959
• UniProt ID: P29965
• Cytogenetics: Xq26.3
• Domains: TNF
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Allograft rejection, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Cytokine-cytokine receptor interaction, Primary immunodeficiency, Systemic lupus erythematosus, T cell receptor signaling pathway, Viral myocarditis
• Summary: The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]