RIC8 (RIC8A) (NM_021932) Human Untagged Clone

SKU SC112883

RIC8A (untagged)-Human resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) (RIC8A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RIC8
• Synonyms: RIC8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112883 sequence for NM_021932 edited (data generated by NextGen Sequencing); ATGGAGCCCCGGGCGGTTGCAGAAGCCGTGGAGACGGGTGAGGAGGATGTGATTATGGAA; GCTCTGCGGTCATACAACCAGGAGCACTCCCAGAGCTTCACGTTTGATGATGCCCAACAG; GAGGACCGGAAGAGACTGGCGGAGCTGCTGGTCTCCGTCCTGGAACAGGGCTTGCCACCC; TCCCACCGTGTCATCTGGCTGCAGAGTGTCCGAATCCTGTCCCGGGACCGCAACTGCCTG; GACCCGTTCACCAGCCGCCAGAGCCTGCAGGCACTAGCCTGCTATGCTGACATCTCTGTC; TCTGAGGGGTCCGTCCCAGAGTCCGCAGACATGGATGTTGTACTGGAGTCCCTCAAGTGC; CTGTGCAACCTCGTGCTCAGCAGCCCTGTGGCACAGATGCTGGCAGCAGAGGCCCGCCTA; GTGGTGAAGCTCACAGAGCGTGTGGGGCTGTACCGTGAGAGGAGCTTCCCCCACGATGTC; CAGTTCTTTGACTTGCGGCTCCTCTTCCTGCTAACGGCACTCCGCACCGATGTGCGCCAG; CAGCTGTTTCAGGAGCTGAAAGGAGTGCGCCTGCTAACTGACACACTGGAGCTGACGCTG; GGGGTGACTCCTGAAGGGAACCCCCCACCCACGCTCCTTCCTTCCCAAGAGACTGAGCGG; GCCATGGAGATCCTCAAAGTGCTCTTCAACATCACCCTGGACTCCATCAAGGGGGAGGTG; GACGAGGAAGACGCTGCCCTTTACCGACACCTGGGGACCCTTCTCCGGCACTGTGTGATG; ATCGCTACTGCTGGAGACCGCACAGAGGAGTTCCAGGGCCACGCAGTGAACCTCCTGGGG; AACTTGCCCCTCAAGTGTCTGGATGTTCTCCTCACCCTGGAGCCACATGGAGACTCCACG; GAGTTCATGGGAGTGAATATGGATGTGATTCGTGCCCTCCTCATCTTCCTAGAGAAGCGT; TTGCACAAGACACACAGGCTGAAGGAGAGTGTAGCTCCCGTGCTGAGCGTGCTGACTGAA; TGTGCCCGGATGCACCGCCCAGCCAGGAAGTTCCTGAAGGCCCAGGNNNNNNNNNNNNCC; CAGGTGCTGCCCCCTCTGCGGGATGTGAGGACACGGCCTGAGGTTGGGGAGATGCTGCGG; AACAAGCTTGTCCGCCTCATGACACACCTGGACACAGATGTGAAGAGGGTGGCTGCCGAG; TTCTTGTTTGTCCTGTGCTCTGAGAGTGTGCCCCGATTCATCAAGTACACAGGCTATGGG; AATGCTGCTGGCCTTCTGGCTGCCAGGGGCCTCATGGCAGGAGGCCGGCCCGAGGGCCAG; TACTCAGAGGATGAGGACACAGACACAGATGAGTACAAGGAAGCCAAAGCCAGCATAAAC; CCTGTGACCGGGAGGGTGGAGGAGAAGCCGCCTAACCCTATGGAGGGCATGACAGAGGAG; CAGAAGGAGCACGAGGCCATGAAGCTGGTGACCATGTTTGACAAGCTCTCCAGGAACAGA; GTCATCCAGCCAATGGGGATGAGTCCCCGGGGTCATCTTACGTCCCTGCAGGATGCCATG; TGCGAGACTATGGAGCAGCAGCTCTCCTCGGACCCTGACTCGGACCCTGACTGA; ; Clone variation with respect to NM_021932.4; 816 c=>g;1067 g=>n;1068 a=>n;1069 t=>n;1070 g=>n;1071 g=>n;1072 c=>n;1073 c=>n;1074 a=>n;1075 c=>n;1076 c=>n;1077 t=>n;1078 c=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021932 unedited; NAAGTGTTCAAATTATGTAATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGAG; GAAGGGCCCGTCCCGCCTTCCCCGGCGCGCCATGGAGCCCCGGGCGGTTGCAGAAGCCGT; GGAGACGGGTGAGGAGGATGTGATTATGGAAGCTCTGCGGTCATACAACCAGGAGCACTC; CCAGAGCTTCACGTTTGATGATGCCCAACAGGAGGACCGGAAGAGACTGGCGGAGCTGCT; GGTCTCCGTCCTGGAACAGGGCTTGCCACCCTCCCACCGTGTCATCTGGCTGCAGAGTGT; CCGAATCCTGTCCCGGGACCGCAACTGCCTGGACCCGTTCACCAGCCGCCAGAGCCTGCA; GGCACTAGCCTGCTATGCTGACATCTCTGTCTCTGAGGGGTCCGTCCCAGAGTCCGCAGA; CATGGATGTTGTACTGGAGTCCCTCAAGTGCCTGTGCAACCTCGTGCTCAGCAGCCCTGT; GGCACAGATGCTGGCAGCAGAGGCCCGCCTAGTGGTGAAGCTCACAGAGCGTGTGGGGCT; GTACCGTGAGAGGAGCTTCCCCCACGATGTCCAGTTCTTTGACTTGCGGCTCCTCTTCCT; GCTAACGGCACTCCGCACCGATGTGCGCCAGCAGCTGTTTCAGGAGCTGAAAGGAGTGCG; CCTGCTAACTGACACACTGGAGCTGACGCTGGGGGTGACTCCTGAAGGGAACCCCCCACC; CACGCTCCTTCCTTCCCAAGAGACTGAGCGGGCCATGGAGATCCTCAAAGTGCTCTTTCA; CATCACCCCTGGACTCCATCAAGGGGGGAGTGGACGANGAAGACGCTGCCCTTTACCGAC; ACCTGGGGGACCCTTCTNNCGCACTGTGTGATGATCGCTACTGCTGGAGACCGCAACNAG; NAGTTCCAGGGCCAGCN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021932 unedited; CCCGCGGGCCGCAATCTAGNAGTCGAGTTTTTTTTTTTTTTTTTTGTCCAAAACTCTTCT; TTATTTCAAGATGATGTTTCTGTGGCTATGTGTGGTATGTGGTATAAATCTCAATCTATG; GTCACACGAGGGGCATTTTCCTTGTTGTAAGTGTAGTCTAAACCAGTAGGAAGGAGGTTT; AATTGCCAAAACCAGCGAGAACTCGGGCACTGTGGATACTACAGTGGGCAGCTGAACGAG; GACCAAGGAGAATGTCTAAGAGGCCTCCAGCCCTGCGCTCAGTGAAGACAGGACAGGAAC; AACAGAGCATACATACCTTGGAAGGGTGTGTTCTGATATACTCGTATGGAAAGTTCTGAC; AGGTTTTCTCCCTGGGAAGTGCAGCACATACCCCAACACACTGGCTCTGCCAGTGTGCCA; ATCCCAAATGGTGCTTGTTTTGTGTGCACCCACACCCAAACCCCTGCCCTCCCTATGCCT; CTTTGTGTGCCCCGGTAGGCCCTCGCCCTATGCTCCACTTTCTGACACATTTCCTCTTGC; GCCACACCATACACAGTTCTTCTCCTTCCGATCTCCCCGCTTGCTTCTGTATCTCTCCCC; CCCCCTATCCCTTCCCTCCCCCACCTTTCCTCCTCTTCCTATAGGCCTTCTTCCCAAACT; CCTTCCCCCTCTTATTAACCGTCCCCTATTTCCTCCAACCCCGCGCACTTTCCTCCCCAC; TCCCCCAATTCCCTCCCCCCCCTTTTATCCCTACTTTCCCCCTACTCCCACTCCTATTTT; CACTTCCCGCTCTCTTTCCTCTCCGTCCTCTCTCATTTCTCCCTCCCTCTCNCACCACCA; TCCCCCCCTTTCCCCACTCTATTTTTTCCCCACCCACTATCTCCCCCCCCCTCTCCATCT; CCACTTCTCCCTCCCTCGTATCGTCACTCCCCTTNTCTCCCCTATCCCTTCTCTACTACA; TCATTTGTATATCTCCCCCTCCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_021932
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021932.4, NP_068751.4
• RefSeq Size: 2714 bp
• RefSeq ORF: 1614 bp
• Locus ID: 60626
• UniProt ID: Q9NPQ8
• Cytogenetics: 11p15.5
• Summary: Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.