RIC8 (RIC8A) (NM_021932) Human Untagged Clone

CAT#: SC112883

RIC8A (untagged)-Human resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) (RIC8A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RIC8
• Synonyms: RIC8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112883 sequence for NM_021932 edited (data generated by NextGen Sequencing)
  ATGGAGCCCCGGGCGGTTGCAGAAGCCGTGGAGACGGGTGAGGAGGATGTGATTATGGAA
  GCTCTGCGGTCATACAACCAGGAGCACTCCCAGAGCTTCACGTTTGATGATGCCCAACAG
  GAGGACCGGAAGAGACTGGCGGAGCTGCTGGTCTCCGTCCTGGAACAGGGCTTGCCACCC
  TCCCACCGTGTCATCTGGCTGCAGAGTGTCCGAATCCTGTCCCGGGACCGCAACTGCCTG
  GACCCGTTCACCAGCCGCCAGAGCCTGCAGGCACTAGCCTGCTATGCTGACATCTCTGTC
  TCTGAGGGGTCCGTCCCAGAGTCCGCAGACATGGATGTTGTACTGGAGTCCCTCAAGTGC
  CTGTGCAACCTCGTGCTCAGCAGCCCTGTGGCACAGATGCTGGCAGCAGAGGCCCGCCTA
  GTGGTGAAGCTCACAGAGCGTGTGGGGCTGTACCGTGAGAGGAGCTTCCCCCACGATGTC
  CAGTTCTTTGACTTGCGGCTCCTCTTCCTGCTAACGGCACTCCGCACCGATGTGCGCCAG
  CAGCTGTTTCAGGAGCTGAAAGGAGTGCGCCTGCTAACTGACACACTGGAGCTGACGCTG
  GGGGTGACTCCTGAAGGGAACCCCCCACCCACGCTCCTTCCTTCCCAAGAGACTGAGCGG
  GCCATGGAGATCCTCAAAGTGCTCTTCAACATCACCCTGGACTCCATCAAGGGGGAGGTG
  GACGAGGAAGACGCTGCCCTTTACCGACACCTGGGGACCCTTCTCCGGCACTGTGTGATG
  ATCGCTACTGCTGGAGACCGCACAGAGGAGTTCCAGGGCCACGCAGTGAACCTCCTGGGG
  AACTTGCCCCTCAAGTGTCTGGATGTTCTCCTCACCCTGGAGCCACATGGAGACTCCACG
  GAGTTCATGGGAGTGAATATGGATGTGATTCGTGCCCTCCTCATCTTCCTAGAGAAGCGT
  TTGCACAAGACACACAGGCTGAAGGAGAGTGTAGCTCCCGTGCTGAGCGTGCTGACTGAA
  TGTGCCCGGATGCACCGCCCAGCCAGGAAGTTCCTGAAGGCCCAGGNNNNNNNNNNNNCC
  CAGGTGCTGCCCCCTCTGCGGGATGTGAGGACACGGCCTGAGGTTGGGGAGATGCTGCGG
  AACAAGCTTGTCCGCCTCATGACACACCTGGACACAGATGTGAAGAGGGTGGCTGCCGAG
  TTCTTGTTTGTCCTGTGCTCTGAGAGTGTGCCCCGATTCATCAAGTACACAGGCTATGGG
  AATGCTGCTGGCCTTCTGGCTGCCAGGGGCCTCATGGCAGGAGGCCGGCCCGAGGGCCAG
  TACTCAGAGGATGAGGACACAGACACAGATGAGTACAAGGAAGCCAAAGCCAGCATAAAC
  CCTGTGACCGGGAGGGTGGAGGAGAAGCCGCCTAACCCTATGGAGGGCATGACAGAGGAG
  CAGAAGGAGCACGAGGCCATGAAGCTGGTGACCATGTTTGACAAGCTCTCCAGGAACAGA
  GTCATCCAGCCAATGGGGATGAGTCCCCGGGGTCATCTTACGTCCCTGCAGGATGCCATG
  TGCGAGACTATGGAGCAGCAGCTCTCCTCGGACCCTGACTCGGACCCTGACTGA
  
  Clone variation with respect to NM_021932.4
  816 c=>g;1067 g=>n;1068 a=>n;1069 t=>n;1070 g=>n;1071 g=>n;1072 c=>n;1073 c=>n;1074 a=>n;1075 c=>n;1076 c=>n;1077 t=>n;1078 c=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021932 unedited
  NAAGTGTTCAAATTATGTAATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGAG
  GAAGGGCCCGTCCCGCCTTCCCCGGCGCGCCATGGAGCCCCGGGCGGTTGCAGAAGCCGT
  GGAGACGGGTGAGGAGGATGTGATTATGGAAGCTCTGCGGTCATACAACCAGGAGCACTC
  CCAGAGCTTCACGTTTGATGATGCCCAACAGGAGGACCGGAAGAGACTGGCGGAGCTGCT
  GGTCTCCGTCCTGGAACAGGGCTTGCCACCCTCCCACCGTGTCATCTGGCTGCAGAGTGT
  CCGAATCCTGTCCCGGGACCGCAACTGCCTGGACCCGTTCACCAGCCGCCAGAGCCTGCA
  GGCACTAGCCTGCTATGCTGACATCTCTGTCTCTGAGGGGTCCGTCCCAGAGTCCGCAGA
  CATGGATGTTGTACTGGAGTCCCTCAAGTGCCTGTGCAACCTCGTGCTCAGCAGCCCTGT
  GGCACAGATGCTGGCAGCAGAGGCCCGCCTAGTGGTGAAGCTCACAGAGCGTGTGGGGCT
  GTACCGTGAGAGGAGCTTCCCCCACGATGTCCAGTTCTTTGACTTGCGGCTCCTCTTCCT
  GCTAACGGCACTCCGCACCGATGTGCGCCAGCAGCTGTTTCAGGAGCTGAAAGGAGTGCG
  CCTGCTAACTGACACACTGGAGCTGACGCTGGGGGTGACTCCTGAAGGGAACCCCCCACC
  CACGCTCCTTCCTTCCCAAGAGACTGAGCGGGCCATGGAGATCCTCAAAGTGCTCTTTCA
  CATCACCCCTGGACTCCATCAAGGGGGGAGTGGACGANGAAGACGCTGCCCTTTACCGAC
  ACCTGGGGGACCCTTCTNNCGCACTGTGTGATGATCGCTACTGCTGGAGACCGCAACNAG
  NAGTTCCAGGGCCAGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021932 unedited
  CCCGCGGGCCGCAATCTAGNAGTCGAGTTTTTTTTTTTTTTTTTTGTCCAAAACTCTTCT
  TTATTTCAAGATGATGTTTCTGTGGCTATGTGTGGTATGTGGTATAAATCTCAATCTATG
  GTCACACGAGGGGCATTTTCCTTGTTGTAAGTGTAGTCTAAACCAGTAGGAAGGAGGTTT
  AATTGCCAAAACCAGCGAGAACTCGGGCACTGTGGATACTACAGTGGGCAGCTGAACGAG
  GACCAAGGAGAATGTCTAAGAGGCCTCCAGCCCTGCGCTCAGTGAAGACAGGACAGGAAC
  AACAGAGCATACATACCTTGGAAGGGTGTGTTCTGATATACTCGTATGGAAAGTTCTGAC
  AGGTTTTCTCCCTGGGAAGTGCAGCACATACCCCAACACACTGGCTCTGCCAGTGTGCCA
  ATCCCAAATGGTGCTTGTTTTGTGTGCACCCACACCCAAACCCCTGCCCTCCCTATGCCT
  CTTTGTGTGCCCCGGTAGGCCCTCGCCCTATGCTCCACTTTCTGACACATTTCCTCTTGC
  GCCACACCATACACAGTTCTTCTCCTTCCGATCTCCCCGCTTGCTTCTGTATCTCTCCCC
  CCCCCTATCCCTTCCCTCCCCCACCTTTCCTCCTCTTCCTATAGGCCTTCTTCCCAAACT
  CCTTCCCCCTCTTATTAACCGTCCCCTATTTCCTCCAACCCCGCGCACTTTCCTCCCCAC
  TCCCCCAATTCCCTCCCCCCCCTTTTATCCCTACTTTCCCCCTACTCCCACTCCTATTTT
  CACTTCCCGCTCTCTTTCCTCTCCGTCCTCTCTCATTTCTCCCTCCCTCTCNCACCACCA
  TCCCCCCCTTTCCCCACTCTATTTTTTCCCCACCCACTATCTCCCCCCCCCTCTCCATCT
  CCACTTCTCCCTCCCTCGTATCGTCACTCCCCTTNTCTCCCCTATCCCTTCTCTACTACA
  TCATTTGTATATCTCCCCCTCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021932
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021932.4, NP_068751.4
• RefSeq Size: 2714 bp
• RefSeq ORF: 1614 bp
• Locus ID: 60626
• UniProt ID: Q9NPQ8
• Cytogenetics: 11p15.5
• Gene Summary: Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.