Translation factor GUF1, mitochondrial (GUF1) (NM_021927) Human Untagged Clone

SKU SC112878

GUF1 (untagged)-Human GUF1 GTPase homolog (S. cerevisiae) (GUF1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Translation factor GUF1, mitochondrial
• Synonyms: DEE40; EF-4; EF4; EIEE40
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021927, the custom clone sequence may differ by one or more nucleotides; ; ; ATGTGGACCCTCGTGGGTCGGGGCTGGGGGTGCGCACGCGCTCTCGCGCCACGAGCCACTGGGGCCGCGC; TTCTGGTGGCCCCGGGGCCCCGGTCCGCGCCGACCCTTGGGGCTGCTCCAGAGTCCTGGGCTACCGACAG; GCTCTACAGCTCCGCAGAATTCAAGGAAAAACTTGACATGTCTAGGTTTCCTGTTGAAAATATTAGAAAT; TTCAGTATTGTTGCACACGTGGATCATGGCAAAAGTACTTTAGCTGACAGGCTCCTAGAACTTACAGGGA; CAATTGATAAAACAAAGAATAATAAGCAGGTTCTTGATAAATTGCAAGTGGAACGAGAAAGAGGAATCAC; TGTTAAAGCACAGACAGCATCTCTCTTTTACAATTGTGAAGGAAAGCAGTACCTTTTAAATCTCATTGAT; ACACCGGGCCATGTTGATTTTAGTTATGAAGTATCCAGGTCACTTTCTGCTTGCCAGGGTGTTTTACTTG; TGGTTGATGCAAATGAGGGAATTCAAGCCCAAACTGTAGCAAACTTCTTTCTTGCCTTCGAAGCACAGCT; ATCGGTAATTCCAGTTATAAATAAGATAGATCTGAAGAATGCTGATCCTGAAAGGGTTGAAAACCAAATT; GAGAAAGTGTTTGATATTCCAAGTGATGAATGTATTAAGATTTCTGCTAAACTTGGAACAAATGTTGAGA; GTGTTCTTCAGGCAATTATTGAAAGAATCCCCCCTCCTAAAGTGCATCGCAAAAATCCTCTGAGAGCTTT; GGTATTTGACTCCACCTTTGACCAGTATAGAGGTGTGATAGCCAATGTAGCATTATTTGACGGAGTGGTT; TCCAAAGGAGATAAAATTGTATCTGCACATACTCAAAAGACATACGAAGTTAATGAAGTAGGAGTCTTGA; ATCCTAATGAGCAGCCAACTCATAAATTATATGCAGGACAGGTGGGCTATCTGATTGCTGGGATGAAAGA; TGTCACTGAAGCGCAAATAGGAGATACATTATGTTTACATAAGCAACCAGTGGAGCCCTTGCCTGGGTTT; AAATCAGCGAAACCAATGGTATTTGCAGGAATGTATCCTCTAGACCAATCTGAATATAACAATCTGAAGA; GTGCTATAGAAAAACTGACTTTAAATGATTCCAGTGTGACCGTTCATCGGGATAGTAGCCTTGCTCTGGG; TGCTGGCTGGAGGCTAGGATTTCTTGGACTTTTGCACATGGAAGTTTTCAACCAGCGACTGGAGCAAGAA; TATAATGCTTCTGTTATTTTAACAACCCCTACTGTTCCATATAAAGCTGTACTGTCATCATCAAAATTGA; TAAAGGAACATAGAGAAAAAGAAATTACAATTATCAATCCTGCACAATTCCCCGATAAATCAAAAGTAAC; AGAATATTTGGAGCCAGTTGTTTTGGGCACTATTATCACACCAGATGAATACACTGGAAAAATAATGATG; CTTTGCGAGGCTCGAAGAGCAGTTCAGAAGAATATGATATTTATTGATCAAAATAGAGTTATGCTTAAAT; ATCTCTTTCCTTTGAATGAAATTGTGGTAGATTTTTATGACTCTTTGAAATCCCTATCTTCTGGATATGC; TAGTTTTGATTACGAAGATGCAGGCTACCAGACTGCAGAACTTGTAAAAATGGATATTCTACTGAATGGA; AATACTGTAGAGGAGCTAGTAACTGTTGTACACAAAGACAAAGCTCATTCAATTGGCAAAGCCATATGTG; AACGGCTGAAGGATTCTCTTCCTAGGCAACTGTTTGAGATAGCAATTCAAGCTGCTATTGGAAGTAAAAT; CATTGCAAGAGAAACTGTGAAAGCCTATAGGAAAAACGTTTTGGCAAAATGTTATGGTGGTGATATTACC; CGAAAAATGAAGCTTTTGAAGAGACAAGCAGAAGGGAAAAAAAAGCTGAGGAAAATTGGCAACGTTGAAG; TTCCAAAAGATGCTTTTATAAAAGTTCTGAAAACACAATCTTCTAAATAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021927 unedited; TTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCGGCACGAGGCCGGATCTGGTACT; TGGGCAGAGCTCCCCGGGGTTCATTGTCTTCGCTTCACAGGATCTGTTTGAGTCCTGTCC; ACCGGATCCTACGGGGGGTACCTTCGAAAAAAAACGGGCTATGCTGCTGTTGCGTGTGGG; TACCCTCTCCTGACGCCTCCGCCGCCCGGGTCATGTGGACCCTCGTGGGTCGGGGCTGGG; GGTGCGCACGCGCTCTCGCGCCACGAGCCACTGGGGCCGCGCTTCTGGTGGCCCCGGGGC; CCCGGTCCGCGCCGACCCTTGGGGCTGCTCCAGAGTCCTGGGCTACCGACAGGCTCTACA; GCTCCGCAGAATTCAAGGAAAAACCTGACATGTCTAGGTTTCCTGTTGAAAATATTAGAA; ATTTCAGTATTGTTGCACACGTGGATCATGGCAAAAGTACTTTAGCTGACAGGCTCCTAG; AACTTACAGGGACAATTGATAAAACAAAGAATAATAAGCAGGTTCTTGATAAATTGCAAG; TGGAACGAGAAAGAGGAATCACTGTTAAAGCACAGACAGCATCTCTCTTTTACAATTGTG; AAGGAAAGCAGTACCTTTTAAATCTCATTGATACACCGGGCCATGTTGATTNTAGTTATG; AAGTATCCAGGTCACTTTCTGCTTGCCAGGGTGTTTTACTTGTGGTTGATGCANATGAGG; GAATTCAAGCCCAACTGTAGCAAACTTCTTTCTTGCCTTCGAAGCACAGCTATCGGTAAT; TCCAGTTATAAATAAGATAGATCCTGAGAATGCTGATCCTGAAAAGTTTGAAAACCAATT; GAGAAAGTGTTTGATATCCAAGTGATGAATGTTTAAGAATTCTGCTTAACTTGGACAAAT; GTTGAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_021927
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021927.1, NP_068746.1
• RefSeq Size: 2671 bp
• RefSeq ORF: 2010 bp
• Locus ID: 60558
• UniProt ID: Q8N442
• Cytogenetics: 4p12
• Domains: EFG_C, GTP_EFTU, GTP_EFTU_D2
• Summary: This gene encodes a GTPase that triggers back-translocation of the elongating ribosome during mitochondrial protein synthesis. The protein contains a highly conserved C-terminal domain not found in other GTPases that facilitates tRNA binding. The encoded protein is thought to prevent misincorporation of amino acids in stressful, suboptimal conditions. An allelic variant in this gene has been associated with early infantile epileptic encephalopathy-40. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]