PHD3 (EGLN3) (NM_022073) Human Untagged Clone

CAT#: SC112867

EGLN3 (untagged)-Human egl nine homolog 3 (C. elegans) (EGLN3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHD3
• Synonyms: HIFP4H3; HIFPH3; PHD3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112867 sequence for NM_022073 edited (data generated by NextGen Sequencing)
  ATGCCCCTGGGACACATCATGAGGCTGGACCTGGAGAAAATTGCCCTGGAGTACATCGTG
  CCCTGTCTGCACGAGGTGGGCTTCTGCTACCTGGACAACTTCCTGGGCGAGGTGGTGGGC
  GACTGCGTCCTGGAGCGCGTCAAGCAGCTGCACTGCACCGGGGCCCTGCGGGACGGCCAG
  CTGGCGGGGCCGCGCGCCGGCGTCTCCAAGCGACACCTGCGGGGCGACCAGATCACGTGG
  ATCGGGGGCAACGAGGAGGGCTGCGAGGCCATCAGCTTCCTCCTGTCCCTCATCGACAGG
  CTGGTCCTCTACTGCGGGAGCCGGCTGGGCAAATACTACGTCAAGGAGAGGTCTAAGGCA
  ATGGTGGCTTGCTATCCGGGAAATGGAACAGGTTATGTTCGCCACGTGGACAACCCCAAC
  GGTGATGGTCGCTGCATCACCTGCATCTACTATCTGAACAAGAATTGGGATGCCAAGCTA
  CATGGTGGGATCCTGCGGATATTTCCAGAGGGGAAATCATTCATAGCAGATGTGGAGCCC
  ATTTTTGACAGACTCCTGTTCTTCTGGTCAGATCGTAGGAACCCACACGAAGTGCAGCCC
  TCTTACGCAACCAGATATGCTATGACTGTCTGGTACTTTGATGCTGAAGAAAGGGCAGAA
  GCCAAAAAGAAATTCAGGAATTTAACTAGGAAAACTGAATCTGCCCTCACTGAAGACTGA
  
  Clone variation with respect to NM_022073.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022073 unedited
  NGGTCGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGCTCGCCGG
  GCCAGTCCGGAACGGGTCGTGGAGCTCCGCACCACTCCCGCTGGTTCCCGAAGGCAGATC
  CCTTCTCCCGAGAGTTGCGAGAAACTTTCCCTTGTCCCCGACGCTGCAGCGGCTCGGGTA
  CCGTGGCAGCCGCAGGTTTCTGAACCCCGGGCCACGCTCCCCGCGCCTCGGCTTCGCGCT
  CGTGTAGATCGTTCCCTCTCTGGTTGCACGCTGGGGATCCCGGACCTCGATTCTGCGGGC
  GAGATGCCCCTGGGACACATCATGAGGCTGGACCTGGAGAAAATTGCCCTGGAGTACATC
  GTGCCCTGTCTGCACGAGGTGGGCTTCTGCTACCTGGACAACTTCCTGGGCGAGGTGGTG
  GGCGACTGCGTCCTGGAGCGCGTCAAGCAGCTGCACTGCACCGGGGCCCTGCGGGACGGC
  CAGCTGGCGGGGCCGCGCGCCGGCGTCTCCAAGCGACACCTGCGGGGCGACCAGATCACG
  TGGATCGGGGGCAACGAGGAGGGCTGCGAGGCCATCAGCTTCCTCCTGTCCCTCATCGAC
  AGGCTGGTCCTCTACTGCGGGAGCCGGCTGGGCAAATACTACGTCAAGGAGAGGTCTAAG
  GCAATGGTGGCTTGCTATCCGGGAAATGGAACAGGTTATGTTCGCCACGTGGACAACCCC
  AACGGTGATGGTCGCTGCATCACCTGCATCTACTATCTGAACAAGAATTGGGATGCCAAG
  CTACATGGTGGNGATCCTGCGGGATATTCCAGAGGGGAAATCATTCATAGCAGATGTGGA
  GCCCATTTTTGACAGACTCCTGTTCTTCTGGTCAGATCGTAGGAACCCACACGAAGTGCA
  GCCCCTCTACGCAACCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022073 unedited
  GGTAAGAGGCTGCCTTCGAGGNNCNGAAAAAATCTTTTTTTTTTTGTGGTGCCATCTTTA
  TTCAGAAGTCATTATTTTAGCACAAGGAGCTGAGGCAGAAAATCCAGGTTGCAGGACTGG
  GAAAAAAGGAGTGTCTGCCCCATGGGACACTTATAAATTGTTCACTATGACAGCAGCCCT
  TTCTAGAGGTATTAATGGCATTAGGTGGTGGTATTCTCCAGAAACCCCAACAGCCCTGGA
  TTAAGAACATCTGGAAATTGTTCAGATGACACAAATTTCTCTGTTTCCTGCTGTTAAGGC
  TTCCGTGATGCCTCTCTGTGGCATCTACCACCTCCATTTTTTTCTTTTCAAACCTCCTCC
  TTTCAAATCAGGAAGTATACATAAAGTGCAAGTAAGGGTCATTCCCTCGCTGTGCTCCTA
  GGCTCTTCTCTTGATAGTATTACCGAATCTATCAGGTAAACCGCTGGCCGAGTAGGATGT
  CTGCAGGAATTTCTGGAGTTAGCAAATAACTTCATCTGGCAAAGAGAGTATCTGAAGATC
  AACACAGTCTTGGCAAGAAAACATGAAGTACCACACACAAGACAGGGATGTGAAGGATGC
  AAGAAGTAGCAGGGAGATTGTTGTCACTGAAGAGGCCATCTTTGGATCTCAAAGAATTTA
  AGAGAATTCAGGAACCGTTACTAAAATGAACAAGGCCAGCAGATTTCAGAGCACGGTCAG
  TCTTCAGTGAGGGCAGATTCAGGTTTCCTAGTTAAATTCCTGGAATTCTCTTTGGCTTCT
  GCCCCTTCTTCAGCATCAAAGTACCAGACAGTCATAGCATATCTGGGTGCGTAAGAAGGC
  TGCACTTCGTGTGGGTCCTACGATCTGACCAGAGAACAGAGTCTGTCCAAATGGGCTCCC
  CATCTGCTATGAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022073
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022073.2, NP_071356.1
• RefSeq Size: 2765 bp
• RefSeq ORF: 720 bp
• Locus ID: 112399
• UniProt ID: Q9H6Z9
• Cytogenetics: 14q13.1
• Domains: 2OG-FeII_Oxy, P4Hc
• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, Renal cell carcinoma
• Gene Summary: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation. Plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway. Target proteins are preferentially recognized via a LXXLAP motif.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).