Glycogen synthase 2 (GYS2) (NM_021957) Human Untagged Clone

SKU SC112826

GYS2 (untagged)-Human glycogen synthase 2 (liver) (GYS2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Glycogen synthase 2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112826 sequence for NM_021957 edited (data generated by NextGen Sequencing); ATGCTTCGAGGCCGATCCCTCTCTGTAACATCCCTGGGTGGGCTTCCCCAGTGGGAAGTC; GAAGAACTTCCTGTGGAGGAGTTACTGCTCTTTGAAGTTGCTTGGGAAGTGACCAATAAA; GTTGGAGGCATCTATACTGTGATTCAGACAAAGGCCAAAACAACAGCAGATGAATGGGGA; GAGAACTATTTTCTGATAGGTCCATATTTTGAGCATAATATGAAGACTCAGGTGGAACAG; TGTGAACCTGTAAATGATGCTGTCAGAAGAGCAGTGGACGCAATGAATAAGCATGGCTGC; CAGGTGCATTTTGGAAGATGGCTGATAGAAGGAAGTCCTTATGTGGTACTTTTTGACATA; GGCTATTCAGCTTGGAATCTGGACAGGTGGAAGGGTGACCTCTGGGAAGCATGCAGTGTC; GGCATTCCTTATCATGACCGAGAAGCCAATGATATGCTGATATTTGGATCTTTAACTGCC; TGGTTCTTAAAAGAGGTGACAGATCATGCAGATGGTAAATATGTCGTTGCCCAATTCCAT; GAATGGCAGGCTGGAATTGGACTGATCCTTTCTCGAGCCAGGAAACTTCCTATTGCCACA; ATATTTACAACCCACGCTACACTACTTGGGAGGTATCTCTGTGCAGCAAATATTGATTTC; TACAACCATCTTGATAAGTTTAACATTGACAAAGAGGCTGGGGAAAGGCAGATTTACCAC; CGGTACTGCATGGAGCGAGCTTCCGTTCATTGCGCTCACGTGTTCACCACGGTTTCTGAA; ATAACAGCAATAGAAGCTGAACATATGCTGAAGAGAAAGCCTGATGTAGTTACTCCAAAC; GGCTTGAATGTTAAGAAATTTTCAGCAGTGCATGAGTTTCAAAATCTACATGCCATGTAC; AAGGCCAGAATCCAAGATTTTGTTCGAGGTCATTTCTATGGTCATCTCGACTTTGATCTT; GAAAAGACTTTGTTCCTTTTCATTGCTGGGAGGTATGAGTTTTCAAACAAAGGAGCTGAC; ATCTTCCTAGAATCCTTATCCAGGCTAAATTTCCTGCTGAGGATGCATAAAAGTGACATC; ACAGTGGTGGTGTTTTTCATTATGCCTGCCAAGACAAATAATTTCAACGTGGAAACCCTG; AAAGGACAAGCAGTGCGAAAACAGCTGTGGGATGTTGCACATTCTGTGAAGGAAAAGTTT; GGAAAAAAACTCTATGATGCATTATTAAGAGGAGAAATTCCTGACCTGAACGATATTTTA; GATCGAGATGATCTAACAATTATGAAAAGAGCCATCTTTTCAACTCAGCGACAGTCATTG; CCCCCAGTGACCACGCACAACATGATTGATGACTCCACCGACCCCATCCTCAGCACCATT; AGACGGATCGGACTTTTCAACAACCGCACAGATAGAGTCAAGGTGATTTTGCACCCAGAG; TTTCTATCCTCCACCAGTCCCTTACTACCCATGGACTATGAAGAGTTTGTTAGAGGTTGT; CATCTTGGAGTATTTCCATCATACTATGAACCCTGGGGTTATACTCCAGCTGAATGCACT; GTGATGGGTATCCCCAGTGTGACCACGAATCTCTCCGGGTTTGGCTGTTTCATGCAGGAG; CACGTGGCTGATCCTACTGCTTACGGTATTTACATCGTTGACAGGCGGTTCCGTTCTCCA; GATGATTCTTGCAATCAGCTGACTAAGTTTCTCTATGGATTTTGCAAACAGTCACGCCGC; CAAAGGATTATCCAGAGGAACAGAACTGAGAGGCTCTCAGATCTTCTGGATTGGAGATAC; TTAGGCAGATATTACCAGCATGCCAGACACCTGACATTAAGCAGAGCTTTTCCAGATAAA; TTCCATGTGGAACTAACATCACCACCAACGACAGAAGGATTTAAATATCCCAGGCCTTCC; TCAGTACCACCTTCTCCTTCAGGGTCTCAGGCCTCCAGTCCTCAGAGCAGTGATGTGGAA; GATGAAGTGGAGGATGAGAGATACGATGAGGAAGAGGAGGCTGAAAGGGATCGGTTAAAT; ATCAAGTCACCATTTTCACTGAGCCACGTTCCTCATGGGAAGAAAAAGCTGCATGGTGAA; TATAAGAACTGA; ; Clone variation with respect to NM_021957.3; 1087 a=>g;1389 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021957 unedited; ATAACGTCGCCAATTGTTAACCATCATATAGGCGGCCCGAATCGCACCGGAAAGAGAGAA; AAAGGAGGAAGATTCCTCCTTCCACCAGGAATTCTGTGGGAAGCACATAAGATTTCATGC; TACTAGTTTATTCCCAAGAGAAGCTACCAAAGCCTGGTAACTCTACCAACTCTAACTTTT; GTGCCTGTAAGTTCTCTTCTCCTGGGATTACAACTAATTGAAACAGGAATTCAAGGAGTC; TCGGAGGACTGTAAGAAGAATGCTTCGAGGCCGATCCCTCTCTGTAACATCCCTGGGTGG; GCTTCCCCAGTGGGAAGTCGAAGAACTTCCTGTGGAGGAGTTACTGCTCTTTGAAGTTGC; TTGGGAAGTGACCAATAAAGTTGGAGGCATCTATACTGTGATTCAGACAAAGGCCAAAAC; AACAGCAGATGAATGGGGAGAGAACTATTTTCTGATAGGTCCATATTTTGAGCATAATAT; GAAGACTCAGGTGGAACAGTGTGAACCTGTAAATGATGCTGTCAGAAGAGCAGTGGACGC; AATGAATAAGCATGGCTGCCAGGTGCATTTTGGAAGATGGCTGATAGAAGGAAGTCCTTA; TGTGGTACTTTTTGACATAGGCTATTCAGCTTGGAATCTGGNACAGGTGGAAAGGTGACC; TCTGGGAAGCATGCAGTGTCNGCATTCCTTATCATGACCGAGNAAGCAATGATATGCTGA; TATTTGGATCTTTTACTGCCTGGTTNCTAAAAGAAGTGACAGATCATGCAGATGGTAAAA; TATGTCGTTGCCCATTCCATGAATGGCAGGCTGGAATTGNACTGATTCTTTCTCGAGCCA; GGAACCTTCTATTGCCCCATTATTTACACCCACGCTACAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021957 unedited; NTTTTACTCTGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTAA; GTTATTAAATATTAATGTGTTTATTTACTTGGATTTTACAAGTTATCATCTATGAAGATT; GACCAAATTTTAAAAAGGCTTTCTATTTAAACAATAAAAAAATGTTACAGTAAGATGGTG; GTACCATGTACATGGTAATATCTTGCATTAATTACAACATCAATTCTAGATTTCAAAAGT; GACATAAATGTCCATTAATATGCATCAAAATATGGACTTAGGATAGCTATTCACTCATGG; ATTTTTGTCTCTGCAAAACTACATCTAAATCATGGTTCTGATGCATGTGAAAAATGAAAA; TAATCAGTAAAAACCTAGCTATTTAAAAAATGTAGGTAGTGCACATTCATGGGTAAGATC; AAGGTTTTCGGGGGGGAAATGGGAAATTTGTGTGGTGGGGTACACAAGCTGCATAAATAG; TAAGCAAAGGATTATGATGATCATTTAAAAATAAACAGAGTAAGAGAAAATCCTTACCAC; TTAAATTCACCATTTTAAAAACACTTTTCCGTCTTTCTGGCCTTTAATGAGTGCTCCTCC; TCATGCCTAACTTTATGGGGGAAACAAGAGTTGGGGAAAATAACTTGGATCTTATCCTAA; ATTACAAAATTATCATTCACTCAATTTCTTCCACTTTTTAGGCAGAGAATAAACTCCATT; GTAATACTTAGAAGGAGAAAATGANATTTGTGGCATTTTTATTTTAAATAATTAGTCTTA; CTTTGCTTTTTTAAATTAGCTCTTCATGCAGCACATGTAGAAATCAGNTCTTATATTCAC; CATGCAGCTTTTTCTTCCATGANGAACGTGNCTAGTGAAAATGTGACTTGATATTAACCG; ATCCCTTNAGNCTGCTCTTCTCACGNATCTCTATCCTCCACTTATCTTCACACACTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_021957
• Insert Size: 2930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021957.2, NP_068776.1
• RefSeq Size: 2912 bp
• RefSeq ORF: 2112 bp
• Locus ID: 2998
• UniProt ID: P54840
• Cytogenetics: 12p12.1
• Protein Pathways: Insulin signaling pathway, Starch and sucrose metabolism
• Summary: The protein encoded by this gene, liver glycogen synthase, catalyzes the rate-limiting step in the synthesis of glycogen - the transfer of a glucose molecule from UDP-glucose to a terminal branch of the glycogen molecule. Mutations in this gene cause glycogen storage disease type 0 (GSD-0) - a rare type of early childhood fasting hypoglycemia with decreased liver glycogen content. [provided by RefSeq, Dec 2009]