CD3G (NM_000073) Human Untagged Clone

SKU SC112775

CD3G (untagged)-Human CD3g molecule, gamma (CD3-TCR complex) (CD3G)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CD3G
• Synonyms: CD3-GAMMA; IMD17; T3G
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112775 sequence for NM_000073 edited (data generated by NextGen Sequencing); ATGGAACAGGGGAAGGGCCTGGCTGTCCTCATCCTGGCTATCATTCTTCTTCAAGGTACT; TTGGCCCAGTCAATCAAAGGAAACCACTTGGTTAAGGTGTATGACTATCAAGAAGATGGT; TCGGTACTTCTGACTTGTGATGCAGAAGCCAAAAATATCACATGGTTTAAAGATGGGAAG; ATGATCGGCTTCCTAACTGAAGATAAAAAAAAATGGAATCTGGGAAGTAATGCCAAGGAC; CCTCGAGGGATGTATCAGTGTAAAGGATCACAGAACAAGTCAAAACCACTCCAAGTGTAT; TACAGAATGTGTCAGAACTGCATTGAACTAAATGCAGCCACCATATCTGGCTTTCTCTTT; GCTGAAATCGTCAGCATTTTCGTCCTTGCTGTTGGGGTCTACTTCATTGCTGGACAGGAT; GGAGTTCGCCAGTCGAGAGCTTCAGACAAGCAGACTCTGTTGCCCAATGACCAGCTCTAC; CAGCCCCTCAAGGATCGAGAAGATGACCAGTACAGCCACCTTCAAGGAAACCAGTTGAGG; AGGAATTGA; ; Clone variation with respect to NM_000073.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000073 unedited; NGGTTCGGGATTAGTATCCGATTCATNATAGGGCGGCCGCGAATTCGGCACCAGGGCTGC; TAAGGGCTGCTCCACGCTTTTGCNCGGAGACAGAGACTGACATGGAACAGGGGAAGGGCC; TGGCTGTCCTCATCCTGGCTATCATTCTTCTTCAAGTACTTTGGCCCAGTCAATCAAAGG; AAACCACTTGGTTAAGGTGTATGACTATCAAGAAGATGGTTCGGTACTTCTGACTTGTGA; TGCAGAAGCCAAAAATATCACATGGTTTAAAGATGGGAAGATGATCGGCTTCCTAACTGA; AGATAAAAAAAAATGGAATCTGGGAAGTAATGCCAAGGACCCTCGAGGGATGTATCAGTG; TAAAGGATCACAGAACAAGTCAAAACCACTCCAAGTGTATTACAGAATGTGTCAGAACTG; CATTGAACTAAATGCAGCCACCATATCTGGCTTTCTCTTTGCTGAAATCGTCAGCATTTT; CGTCCTTGCTGTTGGGGTCTACTTCATTGCTGGACAGGATGGAGTTCGCCAGTCGAGAGC; TTCAGACAAGCAGACTCTGTTGCCCAATGACCAGCTCTACCAGCCCCTCAAGGATCGAGA; AGATGACCAGTACAGCCACCTTCAAGGAAACCAGTTGAGGAGGAATTGAACTCAGGACTC; AGAGTAGTCCAGGTGTTCTCCTCCTATTCAGTTCCCAGAATCAAAAGCATGCATTTTGGA; AAGCTCCTAGCAGAGAGACTTTCAGCCCTAAATCTAGACTCAAGGTTCCCAGAGATGACA; AATGGAGAAGAAAGGCCATCAGAGCAAATTTGGGGTTTCTCAAATAATAAAATAAAACCA; ATCCTGTGTTTCAGAAGCGCACCTATTGNGGAAAATGTNNAAGAAAATGAAAGTTCCATA; ACCCCCTGAAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_000073
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000073.1, NP_000064.1
• RefSeq Size: 822 bp
• RefSeq ORF: 549 bp
• Locus ID: 917
• UniProt ID: P09693
• Cytogenetics: 11q23.3
• Domains: IGc2, ITAM
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage, T cell receptor signaling pathway
• Summary: The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008]