Adenine Nucleotide Translocator 2 (SLC25A5) (NM_001152) Human Untagged Clone

SKU SC112710

SLC25A5 (untagged)-Human solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 5 (SLC25A5), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: Adenine Nucleotide Translocator 2
• Synonyms: 2F1; AAC2; ANT2; T2; T3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112710 sequence for NM_001152 edited (data generated by NextGen Sequencing); ATGACAGATGCCGCTGTGTCCTTCGCCAAGGACTTCCTGGCAGGTGGAGTGGCCGCAGCC; ATCTCCAAGACGGCGGTAGCGCCCATCGAGCGGGTCAAGCTGCTGCTGCAGGTGCAGCAT; GCCAGCAAGCAGATCACTGCAGATAAGCAATACAAAGGCATTATAGACTGCGTGGTCCGT; ATTCCCAAGGAGCAGGGAGTTCTGTCCTTCTGGCGCGGTAACCTGGCCAATGTCATCAGA; TACTTCCCCACCCAGGCTCTTAACTTCGCCTTCAAAGATAAATACAAGCAGATCTTCCTG; GGTGGTGTGGACAAGAGAACCCAGTTTTGGCTCTACTTTGCAGGGAATCTGGCATCGGGT; GGTGCCGCAGGGGCCACATCCCTGTGTTTTGTGTACCCTCTTGATTTTGCCCGTACCCGT; CTAGCAGCTGATGTGGGTAAAGCTGGAGCTGAAAGGGAATTCCGAGGCCTCGGTGACTGC; CTGGTTAAGATCTACAAATCTGATGGGATTAAGGGCCTGTACCAAGGCTTTAACGTGTCT; GTGCAGGGTATTATCATCTACCGAGCCGCCTACTTCGGTATCTATGACACTGCAAAGGGA; ATGCTTCCGGATCCCAAGAACACTCACATCGTCATCAGCTGGATGATCGCACAGACTGTC; ACTGCTGTTGCCGGGTTGACTTCCTATCCATTTGACACTGTTCGCCGCCGCATGATGATG; CAGTCAGGGCGCAAAGGAACTGACATCATGTACACAGGCACGCTTGACTGCTGGCGGAAG; ATTGCTCGTGATGAAGGAGGCAAAGCTTTTTTCAAGGGTGCATGGTCCAATGTTCTCAGA; GGCATGGGTGGTGCTTTTGTGCTTGTCTTGTATGATGAAATCAAGAAGTACACATAA; ; Clone variation with respect to NM_001152.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001152 unedited; ACTAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCAAAGCC; GGTTCCCGGCCCAGTCCCGTCCTGCAGCAGTCTGCCTCCTCTTTCAACATGACAGATGCC; GCTGTGTCCTTCGCCAAGGACTTCCTGGCAGGTGGAGTGGCCGCAGCCATCTCCAAGACG; GCGGTAGCGCCCATCGAGCGGGTCAAGCTGCTGCTGCAGGTGCAGCATGCCAGCAAGCAG; ATCACTGCAGATAAGCAATACAAAGGCATTATAGACTGCGTGGTCCGTATTCCCAAGGAG; CAGGGAGTTCTGTCCTTCTGGCGCGGTAACCTGGCCAATGTCATCAGATACTTCCCCACC; CAGGCTCTTAACTTCGCCTTCAAAGATAAATACAAGCAGATCTTCCTGGGTGGTGTGGAC; AAGAGAACCCAGTTTTGGCTCTACTTTGCAGGGAATCTGGCATCGGGTGGTGCCGCAGGG; GCCACATCCCTGTGTTTTGTGTACCCTCTTGATTTTGCCCGTACCCGTCTAGCAGCTGAT; GTGGGTAAAGCTGGAGCTGAAAGGGAATTCCGAGGCCTCGGTGACTGCCTGGTTAAGATC; TACAAATCTGATGGGATTAAGGGCCTGTACCAAGGCTTTAACGTGTCTGTGCAGGGTATT; ATCATCTACCGAGCCGCCTACTTCGGTATCTATGACACTGCANAGGGAATGCTTCCGGAT; CCCAGAACACTCACATCGTCATCAGCTGGATGATCGCACAGACTGTCCCCTGCTGTGCCC; GGTTGACTNCCTATCCATTTGACACTGTTCGCCGCCGCATGATGATGCAGTCAGGCCGCA; AAGAACTGACATATGTACACAGCACGCCTGACTGCTGCCGGAGAATGCTCGTGATGAAGA; AGCAAAGCTTTTTCAAGGGGCATGTTCCATGTTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001152 unedited; GCAATCTAGAGTCGAGTTCTTTTTTTTTTTTTTTTTTATTTTAGATATTTTTATTTCTCC; AAATTTGTTATTTATCAGGAGTGACTGAAATAAAAATACAATTGAGTCCCATCATCATCA; TCATGGAAATGGCTTTAAGAGAAAACTGGTCAGATGAATATTATTGCTTCCCATTTTCAA; CCAGTAAATAGTTGCCACTGAGAAACTGACAGCCAGGAGTCTGTCAAGAATGCTCAAGAT; ATGTTATATAATACAACATGCCTGTTCACAGGGGGAAAAATCCTAGGAAATAACTTATGT; GTACTTCTTGATTTCATCATACAAGACAAGCACAAAAGCACCACCCATGCCTCTGAGAAC; ATTGGACCATGCACCCTTGAAAAAAGCTTTGCCTCCTTCATCACGAGCAATCTTCCGCCA; GCAGTCAAGCGTGCCTGTGTACATGATGTCAGTTCCTTTGCGCCCTGACTGCATCATCAT; GCGGCGGCGAACAGTGTCAAATGGATAGGAAGTCAACCCGGCAACAGCAGTGACAGTCTG; TGCGATCATCCAGCTGATGACGATGTGAGTGTTCTTGGGATCCGGAAGCATTCCCTCTGC; AGTGTCATAGATACCGAAGTATGCGGCTCGGTAGATGATAATACCCTGCACAGACACGTT; AAAGCCCTGGTACAGGCCCCTTATCCCATCAGATTTGTAGATCCTAACCANGCAGTCACC; GAGGCCCTCGAATTCCCTTTCAGCTCCAGCTTTACCCACATCAGCTGCTAGACGGGTACG; GNNCAAATCAAGATGGTACACANACACAGGGATGGGGCCCCCTGCGCACCACCGATGCCA; GATTCCTGCAAGTATAGCCAAACTGGGTTCTCTTGNCACACCACCCAGAAGATCTGCCTG; ATTATCTTTGAAGGCGAGTTANAACCCCGGTTGGGAATTATCTGATGACATGGCCAGGTT; ACCGGCCAAAGNACGTACTCCTGCTCCTGGGAATCGCACACCATTCTATGCCTTGTATGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_001152
• Insert Size: 1260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001152.1, NP_001143.1
• RefSeq Size: 1225 bp
• RefSeq ORF: 897 bp
• Locus ID: 292
• UniProt ID: P05141
• Cytogenetics: Xq24
• Domains: mito_carr
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway, Huntington's disease, Parkinson's disease
• Summary: This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein forms a homodimer embedded in the inner mitochondria membrane. Suppressed expression of this gene has been shown to induce apoptosis and inhibit tumor growth. The human genome contains several non-transcribed pseudogenes of this gene.[provided by RefSeq, Jun 2013]