14-3-3 epsilon (YWHAE) (NM_006761) Human Untagged Clone

CAT#: SC112695

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YWHAE (untagged)-Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (YWHAE), transcript variant 1


Product Datasheet for 'SC112695'


  "NM_006761" in other vectors (7)

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USD 310.00


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    • 10 ug


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Specifications

Product Data
Product Name 14-3-3 epsilon (YWHAE) (NM_006761) Human Untagged Clone
Type Human Untagged Clone
Tag Tag Free
Symbol YWHAE
Synonyms 14-3-3E; HEL2; KCIP-1; MDCR; MDS
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Cell Selection None
Sequence Data
>OriGene ORF within SC112695 sequence for NM_006761 edited (data generated by NextGen Sequencing)
ATGGATGATCGAGAGGATCTGGTGTACCAGGCGAAGCTGGCCGAGCAGGCTGAGCGATAC
GACGAAATGGTGGAGTCAATGAAGAAAGTAGCAGGGATGGATGTGGAGCTGACAGTTGAA
GAAAGAAACCTCCTATCTGTTGCATATAAGAATGTGATTGGAGCTAGAAGAGCCTCCTGG
AGAATAATCAGCAGCATTGAACAGAAAGAAGAAAACAAGGGAGGAGAAGACAAGCTAAAA
ATGATTCGGGAATATCGGCAAATGGTTGAGACTGAGCTAAAGTTAATCTGTTGTGACATT
CTGGATGTACTGGACAAACACCTCATTCCAGCAGCTAACACTGGCGAGTCCAAGGTTTTC
TATTATAAAATGAAAGGGGACTACCACAGGTATCTGGCAGAATTTGCCACAGGAAACGAC
AGGAAGGAGGCTGCGGAGAACAGCCTAGTGGCTTATAAAGCTGCTAGTGATATTGCAATG
ACAGAACTTCCACCAACGCATCCTATTCGCTTAGGTCTTGCTCTCAATTTTTCCGTATTC
TACTACGAAATTCTTAATTCCCCTGACCGTGCCTGCAGGTTGGCAAAAGCAGCTTTTGAT
GATGCAATTGCAGAACTGGATACGCTGAGTGAAGAAAGCTATAAGGACTCTACACTTATC
ATGCAGTTGTTACGTGATAATCTGACACTATGGACTTCAGACATGCAGGGTGACGGTGAA
GAGCAGAATAAAGAAGCGCTGCAGGACGTGGAAGACGAAAATCAGTGA

Clone variation with respect to NM_006761.4
>OriGene 5' read for NM_006761 unedited
GGCGAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGTCGGAGA
CACTATCCGCTTCCATCCGTCGCGCAGACCCTGCCGGAGCCGCTGCCGCTATGGATGATC
GAGAGGATCTGGTGTACCAGGCGAAGCTGGCCGAGCAGGCTGAGCGATACGACGAAATGG
TGGAGTCAATGAAGAAAGTAGCAGGGATGGATGTGGAGCTGACAGTTGAAGAAAGAAACC
TCCTATCTGTTGCATATAAGAATGTGATTGGAGCTAGAAGAGCCTCCTGGAGAATAATCA
GCAGCATTGAACAGAAAGAAGAAAACAAGGGAGGAGAAGACAAGCTAAAAATGATTCGGG
AATATCGGCAAATGGTTGAGACTGAGCTAAAGTTAATCTGTTGTGACATTCTGGATGTAC
TGGACAAACACCTCATTCCAGCAGCTAACACTGGCGAGTCCAAGGTTTTCTATTATAAAA
TGAAAGGGGACTACCACAGGTATCTGGCAGAATTTGCCACAGGAAACGACAGGAAGGAGG
CTGCGGAGAACAGCCTAGTGGCTTATAAAGCTGCTAGTGATATTGCAATGACAGAACTTC
CACCAACGCATCCTATTCGCTTAGGTCTTGCTCTCAATTNTTCCGTATTCTACTACGAAA
TTCTTAATTCCCCTGACCGTGCCTGCAGGTTGGCAAAAGCAGCTTTTGATGATGNCATTG
CAGAACTGGATACGCTGAGTGAAGAAAGCTATTAGGACTCTACACTTATCATGCAGTTGN
TACGTGATAATCTGACACTATGGACTTCAGACTGCAGGGTGACGGTGAAGAGCANAATAA
AGAGCGCTGCAGGACGTGGAAGACGAAAATCAGTGAGACATAAGCCCACAGAGAAACATC
TCTGACCCCCCCTTCT
>OriGene 3' read for NM_006761 unedited
GCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTAGGTTT
GTTTTATTTAAGTTTAATGTTAATTCCATGCTGGGTTTCAGTAAGAACAATACAGATTCT
GTATCTGTGGCTCCAGTCAGATATCCAGTAGTACAAATTAGCTTCAAGTTACACATACTG
AACAAAAGAGGTTGAGCGAGCGAAGGAGGGGAGGAGTGAGGGGAAGGAGGTAGGGGGAGG
GGGNAANGCCCCNNCCCNNNNNNNTTGNNCNNNCNTGCNNNCTTTTCCNTNCCNCCTTCN
NCGCTTCCCTGCTTTNTTGNCCNNGCCCCGTGGGTCTGCTTGCGCGGCCCCGGNTTGTTC
GTCTGTGTCCNCTNCCTTGCTTTCTGTCCCCCTTGCTTCGGCGGTGCTTTCGTGTTTTTC
GCCGGNCCGTTGCCTCGTCGCCCTTTCTCGNGCTTCCTTGCCCCGTTGGCTTCTTTCGCC
CCTTGTCTTGCCTGGCGGCCTGGCCCCCCGGCCTCCCCTTTCTGNTCCTTGCCTCCTGCT
TCCCCTTTGCCTCTTTCGGTCGTTCCCCCGTTTCTCCCTGGGGCTTCTTNTGTGGTCTTT
TGGCTCGCGCCCGTTCTCTCCCTTCGCTGTTGTTCCCTTTGCTCCTTTGTCCCGGGTTTG
ATCGCTCTCTGTTTNCCCCGCTCTGTTTTTTCTGCCTTCGCCTCTTGCCTCTTTCGCTGT
GCCCCGCNTGTCTTCCGTGGTCCCTCTTGCTTCTGCGCTCTCGTCTCTTTGTCTCTCCCC
TTGTGTCTCTCCCGCCGCNTTTCTTTCTTCCCCTTCTCCTCTTTTTTTGTCCTCTCCTTT
CTTTGGTCTCCGGCTGGCTGCTTCTCCCGCTGGTGCCCTGCNTTCGCCCGTACTGGTTAC
TCTTGGTTCAGGTCTCCCCTCTT
Restriction Sites NotI-NotI     
Tag Tag Free
ACCN NM_006761
Insert Size 1770 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006761.3, NP_006752.1
RefSeq Size 1810 bp
RefSeq ORF 768 bp
Locus ID 7531
Cytogenetics 17p13.3
Domains 14-3-3
Protein Families Druggable Genome
Protein Pathways Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis
Gene Summary This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]
Transcript Variant: This variant (1) represents the protein-coding transcript.

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