DNA polymerase delta p50 (POLD2) (NM_006230) Human Untagged Clone

SKU SC112691

POLD2 (untagged)-Human polymerase (DNA directed), delta 2, regulatory subunit 50kDa (POLD2), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: DNA polymerase delta p50
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112691 sequence for NM_006230 edited (data generated by NextGen Sequencing); ATGTTTTCTGAGCAGGCTGCCCAGAGGGCCCACACTCTACTGTCCCCACCATCAGCCAAC; AATGCCACCTTTGCCCGGGTGCCAGTGGCAACCTACACCAACTCCTCACAACCCTTCCGG; CTAGGAGAGCGCAGCTTTAGCCGGCAGTATGCCCACATTTATGCCACCCGCCTCATCCAA; ATGAGACCCTTCCTGGAGAACCGGGCCCAGCAGCACTGGGGCAGTGGAGTGGGAGTGAAG; AAGCTGTGTGAACTGCAGCCTGAGGAGAAGTGCTGTGTGGTGGGCACTCTGTTCAAGGCC; ATGCCGCTGCAGCCCTCCATCCTGCGGGAGGTCAGCGAGGAGCACAACCTGCTCCCCCAG; CCTCCTCGGAGTAAATACATACACCCAGATGACGAGCTGGTCTTGGAAGATGAACTGCAG; CGTATCAAACTAAAAGGCACCATTGACGTGTCAAAGCTGGTTACGGGGACTGTCCTGGCT; GTGTTTGGCTCCGTGAGAGACGACGGGAAGTTTCTGGTGGAGGACTATTGCTTTGCTGAC; CTTGCTCCCCAGAAGCCCGCACCCCCACTTGACACAGATAGGTTTGTGCTACTGGTGTCC; GGCCTGGGCCTGGGTGGCGGTGGAGGCGAGAGCCTGCTGGGCACCCAGCTGCTGGTGGAT; GTGGTGACGGGGCAGCTTGGGGACGAAGGGGAGCAGTGCAGCGCCGCCCACGTCTCCCGG; GTTATCCTCGCTGGCAACCTCCTCAGCCACAGCACCCAGAGCAGGGATTCTATCAATAAG; GCCAAATACCTCACCAAGAAAACCCAGGCAGCCAGCGTGGAGGCTGTTAAGATGCTGGAT; GAGATCCTCCTGCAGCTGAGCGCCTCAGTGCCCGTGGACGTGATGCCAGGCGAGTTTGAT; CCCACCAATTACACGCTCCCCCAGCAGCCCCTCCACCCCTGCATGTTCCCGCTGGCCACT; GCCTACTCCACGCTCCAGCTGGTCACCAACCCCTACCAGGCCACCATTGATGGAGTCAGA; TTTTTGGGGACATCAGGACAGAACGTGAGTGACATTTTCCGATACAGCAGCATGGAGGAT; CACTTGGAGATCCTGGAGTGGACCCTGCGGGTCCGTCACATCAGCCCCACAGCCCCTGAC; ACTCTAGGTTGTTACCCCTTCTACAAAACTGACCCGTTCATCTTCCCAGAGTGCCCGCAT; GTCTACTTTTGTGGCAACACCCCCAGCTTTGGCTCCAAAATCATCCGAGGTCCTGAGGAC; CAGACAGTGCTGTTGGTGACTGTCCCTGACTTCAGTGCCACGCAGACCGCCTGCCTTGTG; AACCTGCGCAGCCTGGCCTGCCAGCCCATCAGCTTCTCGGGCTTCGGGGCAGAGGACGAT; GACCTGGGAGGCCTGGGGCTGGGCCCCTGA; ; Clone variation with respect to NM_006230.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006230 unedited; TTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGCGGGATGCGGCGCGCCGC; GCGTGAACCTCCTTGGCCTGGGCGAAGCTGTGTGGACCAAGCAAGTCAGGAGTGTGGCCA; TGTTTTCTGAGCAGGCTGCCCAGAGGGCCCACACTCTACTGTCCCCACCATCAGCCAACA; ATGCCACCTTTGCCCGGGTGCCAGTGGCAACCTACACCAACTCCTCACAACCCTTCCGGC; TAGGAGAGCGCAGCTTTAGCCGGCAGTATGCCCACATTTATGCCACCCGCCTCATCCAAA; TGAGACCCTTCCTGGAGAACCGGGCCCAGCAGCACTGGGGCAGTGGAGTGGGAGTGAAGA; AGCTGTGTGAACTGCAGCCTGAGGAGAAGTGCTGTGTGGTGGGCACTCTGTTCAAGGCCA; TGCCGCTGCAGCCCTCCATCCTGCGGGAGGTCAGCGAGGAGCACAACCTGCTCCCCCAGC; CTCCTCGGAGTAAATACATACACCCAGATGACGAGCTGGTCTTGGAAGATGAACTGCAGC; GTATCAAACTAAAAGGCACCATTGACGTGTCAAAGCTGGTTACGGNGACTGTCCTGGCTG; TGTTTGGCTCCGTGAGAGACGACGGGAAGTTTCTGGTGGAGGACTATTGCTTTGCTGACC; TTGCTCCCANAAGCCCGCACCCNCACTTGACACAGATAGGTTTGTGCTACTGGTGTCCGG; CCTGGGCCTNGGTGGGCGGTGGAGCAAAAGCCTGCTGGGACCCANCTGCTGTTGGATGTG; GTGACGGGGCATCTGGGGGACGAAGGGGACAGTGCACGCCCGCCACGTCTCCGGGTATCC; TCGTTGGCACCTCCTCACCACAGCACCAAAGAAGGGATCTATCAATAGGCCAATACCTAA; CAAAAAACCCAGCAGCAGGGGGAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006230 unedited; TGGACCGCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCGCATCAGCAAGT; GCTCAGGCTTTATTTACAATGCCGACACTGCAGGGAATGAACAGCCTCCATCTGGGCCTC; TCTGGTCAAAACCACTTTTTTGAGTCAGGGGCCCAGCCCCAGGCCTCCCAGGTCATCGTC; CTCTGCCCCGAAGCCCGAGAAGCTGATGGGCTGGCAGGCCAGGCTGCGCAGGTTCACAAG; GCAGGCGGTCTGCGTGGCACTGAAGTCAGGGACAGTCACCAACAGCACTGTCTGGTCCTC; AGGACCTCGGATGATTTTGGAGCCAAAGCTGGGGGTGTTGCCACAAAAGTAGACATGCGG; GCACTCTGGGAAGATGAACGGGTCAGTTTTGTAGAAGGGGTAACAACCTATAGTGTCAGG; GGCTGTGGGGCTGATGTGACGGACCCGCAGGGTCCACTCCAGGATCTCCAAGTGATCCTC; CATGCTGCTGTATCGGAAAATGTCACTCACGTTCTGTCCTGATGTCCCCAAAAATCTGAC; TCCATCAATGGTGGCCTGGTAGGGGTTTGGTGACCACCTGGAGCGTGGAGTTTGCTATTG; CTCACTGGTAACATGCAAGGGCTGGAGGCCTTGCTGGGGGACCGTTTTATTGGTGGTCCG; AACCAGCTGTGCCGTGACGGCCCACCTACATTGGGCTACTTACCATCGCTCGCCCCCCTT; TGCTCCCGTCCCCATATCCCACTCACTCTTCCGACTTGAACGGACAGCTCCCCCGCCCCG; CGTGTCGTCAATAAATGTGATGCCCCTACCGCGCAGACCGCTTGTTTTTTTCACCTATAA; CCCTAGTTATAGCGTATCACGCTCTCCGAATCCCACTGTGTCTTTTCTTCTTCTTTTGTG; TATTCGTCCCCGCGCCCACTGCCCGCCCCCTCTCGCCCTCCCCAACTGTTTCTCCGGTTG; TCTTTCTGACCCCCCGCGACTCTTCTCTGTCCCGATTAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_006230
• Insert Size: 1510 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006230.1, NP_006221.1
• RefSeq Size: 1584 bp
• RefSeq ORF: 1410 bp
• Locus ID: 5425
• UniProt ID: P49005
• Cytogenetics: 7p13
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Base excision repair, DNA replication, Homologous recombination, Metabolic pathways, Mismatch repair, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism
• Summary: This gene encodes the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA). Expression of this gene may be a marker for ovarian carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Mar 2012]; Transcript Variant: This variant (2) uses an alternate splice site in the 5' region and initiates translation at an upstream start codon, compared to variant 1. The encoded isoform (2) has a longer N-terminus, compared to isoform 1.