ARPC2 (NM_005731) Human Untagged Clone

CAT#: SC112686

ARPC2 (untagged)-Human actin related protein 2/3 complex, subunit 2, 34kDa (ARPC2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARPC2
• Synonyms: ARC34; p34-Arc; PNAS-139; PRO2446
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005731 edited
  GAATTCGGCACGAGGTAGAGCCGGGGGCTGGGCGGGGACCGGGCTTGTCGGTGAAGCGGC
  AGTGGCGGCGGCGGCGGCGGCTCGGCAGGCGGGTTCAGGCTTCGGGGGCCAGCCGCCGCC
  ATGATCCTGCTGGAGGTGAACAACCGCATCATCGAGGAGACGCTCGCGCTCAAGTTCGAG
  AACGCGGCCGCCGGAAACAAACCGGAAGCAGTAGAAGTAACATTTGCAGATTTCGATGGG
  GTCCTCTATCATATTTCAAATCCTAATGGAGACAAAACAAAAGTGATGGTCAGTATTTCT
  TTGAAATTCTACAAGGAACTTCAGGCACATGGTGCTGATGAGTTATTAAAGAGGGTGTAC
  GGGAGTTTCTTGGTAAATCCAGAATCAGGATACAATGTCTCTTTGCTATATGACCTTGAA
  AATCTTCCGGCATCCAAGGATTCCATTGTGCATCAAGCTGGCATGTTGAAGCGAAATTGT
  TTTGCCTCTGTCTTTGAAAAATACTTCCAATTCCAAGAAGAGGGCAAGGAAGGAGAGAAC
  AGGGCAGTTATCCATTATAGGGATGATGAGACCATGTATGTTGAGTCTAAAAAGGACAGA
  GTCACAGTAGTCTTCAGCACAGTGTTTAAGGATGACGACGATGTGGTCATTGGAAAGGTG
  TTCATGCAGGAGTTCAAAGAAGGACGCAGAGCCAGCCACACAGCCCCACAGGTCCTCTTT
  AGCCACAGGGAACCTCCTCTGGAGCTGAAAGACACAGACGCCGCTGTGGGTGACAACATT
  GGCTACATTACCTTTGTGCTGTTCCCTCGTCACACCAATGCCAGTGCTCGAGACAACACC
  ATCAACCTGATCCACACGTTCCGGGACTACCTGCACTACCACATCAAGTGCTCTAAGGCC
  TATATTCACACACGTATGCGGGCGAAAACGTCTGACTTCCTCAAGGTGCTGAACCGCGCA
  CGCCCAGATGCCGAGAAAAAAGAAATGAAAACAATCACGGGGAAGACGTTTTCATCCCGC
  TAATCTTGGGAATAAGAGGAGGAAGCGGCTGGCAACTGAAGGCTGGAACACTTGCTACTG
  GATAATCGTAGCTTTTAATGTTGCGCCTCTTCAGGTTCTTAAGGGATTCTCCGTTTTGGT
  TCCATTTTGTACACGTTTGGAAAATAATCTGCAGAAACGAGCTGTGCTTGCAAAGACTTC
  ATAGTTCCCAAGAATTAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005731 unedited
  GACTCGGGATTTGTAACCGACTTACTTAGGGCGGCCACGCAATTCGCACGAGGTAGAGCC
  GGTGGCTGGGCGGGGACCGGGCTTGTCGGTGAAGCGGCAGTGGCGGCGGCGGCGGCGGCT
  CGGCAGGCGGGTTCAGGCTTCGGGGGCCAGCCGCCGCCATGATCCTGCTGGAGGTGAACA
  ACCGCATCATCGAGGAGACGCTCGCGCTCAAGTTCTATAACGCGGCCGCCGGAAACAAAC
  CGGAAGCAGTAGAAGTAACATTTGCAGATTTCGATGGGGTCCTCTATCATATTTCAAATC
  CTAATGGAGACAAAACAAAAGTGATGGTCAGTATTTCTTTGAAATTCTACAAGGAACTTC
  AGGCACATGGTGCTGATGAGTTATTAAAGAGGGTGTACGGGAGTTTCTTGGTAAATCCAG
  AATCAGGATACAATGTCTCTTTGCTATATGACCTTGAAAATCTTCCGGCATCCAAGGATT
  CCATTGTGCATCAAGCTGGCATGTTGAAGCGAAATTGTTTTGCCTCTGTCTTTGAAAAAT
  ACTTCCAATTCCAAGAAGAGGGCAAGGAAGGAGAGAACAGGGCAGTTATCCATTATAGGG
  ATGATGAGACCATGTATGTTGAGTCTAAAAAGGACAGAGTCACAGTAGTCTTCAGCACAG
  TGTTTAAGGATGACGACGATGTGGTCATTGGAAAGGTGTTCATGCAGGAGTTCAAAGAAG
  GACGCAGAGCCAGCCACACAGCCCCACAGGTCCTCTTTAGCCACAGGGAACCTCCTCTGG
  AGCTGANAGACACAGACGCCGCTGTGGGTGACAACATTGCTACATTACCTTTGTGCTGTT
  TCCTCGTCACACCAATGCCAGTGCTCGAGACCACACCATCACCTGATCCACACGTTCCGG
  GACTACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005731 unedited
  CGCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTCTTGGGAACTATGA
  AGTCTTTGCAAGCACAGCTCGTTTCTGCAGATTATTTTCCAAACGTGTACAAAATGGAAC
  CAAAACGGAGAATCCCTTAAGAACCTGAAGAGGCGCAACATTAAAAGCTACGATTATCCA
  GTAGCAAGTGTTCCAGCCTTCAGTTGCCAGCCGCTTCCTCCTCTTATTCCCAAGATTAGC
  GGGATGAAAACGTCTTCCCCGTGATTGTTTTCATTTCTTTTTTCTCGGCATCTGGGCGTG
  CGCGGTTCAGCACCTTGAGGAAGTCAGACGTTTTCGCCCGCATACGTGTGTGAATATAGG
  CCTTAGAGCACTTGATGTGGTAGTGCAGGTAGTCCCGGAACGTGTGGATCAGGTTGATGG
  TGTTGTCTCGAGCACTGGCATTGGTGTGACGAGGGAACAGCACAAAGGTAATGTAGCCAA
  TGTTGTCACCCACAGCGGCGTCTGTGTCTTTCAGCTCCAGAGGAGGTTCCCTGTGGCTAA
  AGAGGACCTGTGGGGCTGTGTGGCTGGCTCTGCGTCCTTCTTTGAACTCCTGCATGAACA
  CCTTTCCAATGACCACATCGTCGTCATCCTTAAACACTGTGCTGAAGACTACTGTGACTC
  TGTCCTTTTTAGACTCAACATACATGGTCTCATCATCCCTATAATGGATAACTGCCCTGT
  TCTCTCCTTCCTTGCCCTCTTCTTGGAATTGGAAGTATTTTTCAAAGAACAGAGGCAAAC
  AATTTCGCTTCACATGCCAGCTTGATGCACAATGGGATCCTTGGATGCCGGNAAGATTTC
  AGGTCATATAGCANAGAACATGTATCCTGATTCTGGATTACCCAGAACTNCGTACACCTC
  TTTATAACTACAGCACATGTGCCTGAGNTCTTGTAGATTCAAGAAACTGACATACTTTGT
  TT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005731
• Insert Size: 1240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005731.2, NP_005722.1
• RefSeq Size: 1424 bp
• RefSeq ORF: 903 bp
• Locus ID: 10109
• UniProt ID: O15144
• Cytogenetics: 2q35
• Domains: p34-Arc
• Protein Pathways: Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
• Gene Summary: This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.