FAM35A (NM_019054) Human Untagged Clone

SKU SC112592

FAM35A (untagged)-Human family with sequence similarity 35, member A (FAM35A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: FAM35A
• Synonyms: bA163M19.1; FAM35A; FAM35A1; RINN2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112592 sequence for NM_019054 edited (data generated by NextGen Sequencing); ATGAGTGGAGGATCTCAAGTCCACANNTTTTGGGGTGCTCCAATTGCTCCACTGAAAATC; ACAGTATCAGAAGACACAGCTTCTTTAATGTCTGTTGCTGACCCCTGGAAAAAAATTCAG; CTTTTATACAGTCAACATTCTTTATATCTGAAGGATGAAAAACAGCACAAAAATCTTGAA; AACTATAAAGTCCCAGAATCTATTGGTTCTCCAGATCTTAGTGGTCATTTCTTAGCAAAC; TGTATGAATAGACATGTTCATGTGAAAGATGACTTTGTACGTTCTGTTTCTGAAACACAG; AATATAGAATCCCAGAAGATTCACTCCTCTAGACTGAGTGATATAACTAGCTCTAATATG; CAAATATGTGGATTTAAAAGCACAGTTCCGCATTTCACCGAAGAAGAAAAGTATCAAAAG; CTTCTCAGTGAAAATAAAATTAGAGATGAACAGCCTAAACATCAGCCAGATATATGTGGT; AAGAACTTTAACACAAATTTGTTTCAGTTGGGCCATAAATGTGCAGCTGTGTTGGATTTG; GTTTGTAGTACTGAAAAAATTAATATAGGGCCTGAAGTGGTACAAAGAGAGTGTGTGCCA; ACAGAATATCATGAAATACAAAACCAGTGTTTGGGATTATTTTCCTCGAACGCAGTAGAT; AAGTCAAGGTCTGAAGCAGCAGTTAGGAAGGTCTCAGACCTTAAAATATCAACTGATACA; GAATTTCTCAGTATAATTACCTCCAGCCAGGTTGCTTTTTTAGCTCAAAAGAAAGATAAA; AGGCGGAGTCCTGTAAATAAAGGGAATGTAAACATGGAGACTGAACCAAAGGCAAGTTAC; GGGGAGATAAGAATACCTGAAGAGAATTCGATTCAGCTTGATGGTTTTACAGAAGCATAT; GAAAGTGGACAAAACCAAGCATATTCCCTTGAACTTTTTAGTCCTGTTTGTCCTAAAACA; GAAAATAGCCGCATTCACATAAACTCTGATAAAGGTCTTGAAGAACATACAGGATCTCAA; GAACTTTTCAGTTCTGAAGATGAACTGCCACCAAATGAGATACGTATTGAGTTGTGTAGC; TCAGGAATACTGTGTTCCCAACTAAATACCTTCCACAAAAGTGCTATTAAAAGAAGCTGT; ACCTCTGAAGATAAAGTGGGCCAGTCTGAAGCTCTATCTAGAGTCCTTCAAGTAGCTAAG; AAAATGAAGTTGATTTCTAATGGAGGAGATTCTGCTGTAGAAATGGATCGGAGAAATGTG; TCTGAATTTAAGAGTATTAAAAAAACATCATTAATAAAAAACTGTGATTCTAAAAGCCAG; AAGTATAATTGTTTAGTCATGGTGCTATCTCCATGCCATGTGAAGGAAATAAACATAAAA; TTCGGACCAAATTCTGGCTCTAAAGTGCCTTTAGCAACAGTTACAGTAATTGATCAATCA; GAAACTAAGAAGAAGGTTTTTCTGTGGAGGACTGCAGCATTTTGGGCATTTACAGTGTTT; CTTGGAGATATAATTTTACTCACAGATGTTGTTATTCATGAGGACCAATGGATTGGCGAG; ACAGTACTACAATCAACATTTAGCAGTCAGTTATTAAATCTTGGGAGTTATTCATCTATT; CAGCCTGAAGAATATTCCAGTGTAGTTAGTGAAGTTGTACTTCAAGACTTACTGGCATAT; GTGTCCTCAAAACATTCCTACCTCAGAGATCTTCCTCCGAGGCAGCCTCAGAGGGTGAAC; AGTATAGACTTTGTAGAATTGGAGCACCTTCAACCTGATGTATTAGTCCACGCAGTACTA; AGAGTTGTTGATTTCACTATACTGACAGAGGCAGTATACAGTTATAGAGGACAGAAGCAG; AAAAAAGTTATGTTAACAGTGGAACAGGCCCAAGATCAACATTATGCGCTTGTATTATGG; GGTCCTGGAGCAGCCTGGTACCCTCAACTTCAAAGGAAAAAAGGAGTGGTTCTGATTAAA; GCCCAGATTTCAGAGCTGGCATTTCCTATTACAGCATCTCAGAAGATAGCGCTAAATGCT; CACAGTTCTCTGAAGAGTATTTTTTCTTCTCTTCCCAACATCGTATATACTGGCTGTGCA; AAATGTGGATTGGAACTAGAAACAGATGAGAACAGGATCTACAAACAATGTTTTAGCTGC; TTGCCATTTACTATGAAGAAAATATATTATAGGCCAGCGTTAATGACTGCCATTGATGGA; AGACATGATGTTTGTATCCGTGTAGAATCAAAGCTGATAGAGAAGATTCTTCTCAACATT; TCTGCAGACTGCCTCAACAGAGTGATAGTTCCTTCCTCAGAGATCACCTATGGGATGGTC; GTGGCAGACCTGTTCCACTCCTTGTTGGCAGTCAGCGCAGAACCTTGTGTATTAAAGATT; CAGAGCCTTTTTGTGTTAGATGAAAACAGCTATCCATTACAACAAGATTTCTCCCTCCTG; GATTTTTATCCTGACATTGTAAAGCATGGAGCCAATGCCCGTCTCTGA; ; Clone variation with respect to NM_019054.2; 26 t=>n;27 t=>n;2094 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_019054 unedited; CGTCACATTTGTATACGACTCATATAGGCGGCCGCGCAATCGGCACGAGGCTGACCTGAA; TCCTGAATTTTCCTTGATGGTCAAAAGATTAAGATGGAGTCTCACTCTGTTGCCCAGGCT; GGAGTGCAATGGCGCGATCTTGGCTCACTGCAACCTCTGCCTTCCGGGTTCAGGCGAGTC; TCCTGCTCCAGCCTCCTGAGTAGCTGGGATTACAGAAATCATGAGTGGAGGATCTCAAGT; CCACATTTTTGGGGTGCTCCAATTGCTCCACTGAAAATCACAGTATCAGAAGACACAGCT; TCTTTAATGTCTGTTGCTGACCCCTGGAAAAAAATTCAGCTTTTATACAGTCAACATTCT; TTATATCTGAAGGATGAAAAACAGCACAAAAATCTTGAAAACTATAAAGTCCCAGAATCT; ATTGGTTCTCCAGAGCTTAGTGGTCATTTCTTAGCAAACTGTATGAATAGACATGTTCAT; GTGAAAGATGACTTTGTACGTTCTGTTTCTGAAACACAGAATATAGAATCCCAGAAGAGT; CACTCCTCTAGACTGAGTGATATAACTAGCTCTAATATGCAAATATGTGGATTTAAAAGC; ACAGTTCCGCATTTCACCGAAGAAGAAAAGTATCAAAAGCTTCTCAGTGAAAATAAAATT; AGAGATGAACAGCCTAAACATCAGCCAGATATATGTGGTAAGAACTTTAACACAAATTTG; TTTCAGTTGGGCCATAAATGTGCAGCTGTGTTGCATTTGGTTTGTAGTACTGAAAAAATA; ATATAGGGCCCTGAGTGGTACACAGAGAGTGTGTGCCAACAGAATATCATGAGATACANA; ACCCAGTGTTTGGGAATATTTTTCTCGAACGCAGTAGATAAGTCAAGGTCTGAAGCAGCA; GTTAGGGAAGGTCTCAGACCTTACCAAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_019054 unedited; CCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCATGGC; ATCATGAATTTATTTTCAGGCAATATGATAATCTATTAACACTCAGCATACTCTGAACTT; ACAGAATAAGTACCACGCCATACTTCCTCATTAATGACTATCATTTTACATGAGCTTCTT; CCACAATCACAATGAGAGAACAGATTGCTACATGTTTGCTGGTAAATGCATTCAGTAAGG; CACTCAAATCCTAGACACGAACTAACCACAGAACGCTCTTCCCTGCTTTAATAAATTGAA; ACATGATCTCATTTCCTTCATTTTCAATACTTTTTATAAAAAGACACGCTTGTCTTGAAC; TTGTCTAAACATAGGTTTATATAAACTAATCCATTATCATTATAGTTATAATCAAGGAAA; TGATTTCACATTGACGGTTTTTTAGGTAGGGATAAGCGAAAGCACTGCAGCAGTTTCTGA; GGGAATGTCTTCTCTGTGGTTCCCCATACTTAGTACAATGCCTGGAATGTACCAGGGACT; AAATACAGTGAACAAATGGGCAAACCTTTAATTCCCAGGGATCTGCTACATATTGCCTTG; ACTGGTAGATCATTTCTCCAGGAGAAAGGAAGAAACCAAAAGACAAAAACATTCTTTTTA; AACCTCACAGGATTTTCTTAGAAAAAAGTTTTAATTTATATATCTAGTGCAATTCTCTTT; GTAAAATATATTCATAAAAAATCAAAGCAAAGCCCTGTATTCATTAATTCAAAAGACAAT; CATTCAGTACTTCTCCTTGAAATGCTGCATTTCTTTCTTTGCCTCAAAGAGGGCATTGGC; TCATGCTTTACATGTCAGGATAAAAATCAGGAGGAAAAATCTTGTGTATGGAAAACTGTT; TCATCTACCAAAAGTTTTGATTCTTATACCAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_019054
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_019054.1, NP_061927.1
• RefSeq Size: 3402 bp
• RefSeq ORF: 2508 bp
• Locus ID: 54537
• UniProt ID: Q86V20
• Cytogenetics: 10q23.2
• Summary: Component of the shieldin complex, which plays an important role in repair of DNA double-stranded breaks (DSBs) (PubMed:29656893, PubMed:29789392). During G1 and S phase of the cell cycle, the complex functions downstream of TP53BP1 to promote non-homologous end joining (NHEJ) and suppress DNA end resection (PubMed:29656893, PubMed:29789392). Mediates various NHEJ-dependent processes including immunoglobulin class-switch recombination, and fusion of unprotected telomeres (PubMed:29656893).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.