RAB3IP (NM_022456) Human Untagged Clone

SKU SC112519

RAB3IP (untagged)-Human RAB3A interacting protein (rabin3) (RAB3IP), transcript variant alpha 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: RAB3IP
• Synonyms: RABIN3; RABIN8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112519 sequence for NM_022456 edited (data generated by NextGen Sequencing); ATGGCTAATGATCCCTTGGAAGGCTTCCATGAAGTAAACCTTGCTTCACCTACTTCTCCG; GACCTTCTTGGTGTGTATGAATCAGGAACTCAAGAGCAGACTACCTCACCAAGTGTCATC; TACCGGCCACACCCTTCAGCTTTATCCTCTGTACCTATCCAGGCAAATGCATTAGATGTT; TCTGAACTTCCTACACAACCCGTGTATTCATCCCCCAGACGTTTAAATTGTGCGGAAATA; TCTAGTATCAGCTTTCATGTTACAGACCCAGCCCCTTGCTCTACCTCTGGAGTCACAGCT; GGATTAACTAAATTAACTACAAGAAAGGACAACTATAATGCAGAGAGAGAGTTTTTACAG; GGTGCTACTATAACAGAGGCTTGCGATGGCAGTGATGATATTTTTGGGTTGAGTACTGAT; AGTCTGTCTCGTTTACGAAGCCCATCTGTTTTGGAAGTTAGAGAAAAGGGCTATGAACGA; TTAAAAGAAGAACTCGCAAAAGCTCAGAGGGAACTGAAGTTAAAAGATGAAGAATGTGAG; AGGCTTTCAAAAGTGCGAGATCAACTTGGACAGGAATTGGAAGAACTCACAGCTAGTCTA; TTTGAGGAAGCTCATAAAATGGTGAGAGAAGCAAATATCAAGCAGGCAACAGCAGAAAAA; CAGCTAAAAGAAGCACAAGGAAAAATTGATGTACTTCAAGCTGAAGTAGCTGCATTGAAG; ACACTTGTATTGTCCAGTTCTCCAACATCACCTACGCAGGAGCCTTTGCCAGGTGGAAAG; ACACCTTTTAAAAAGGGGCATACAAGAAATAAAAGCACAAGCAGTGCTATGAGTGGCAGT; CATCAGGACCTCAGTGTGATACAGCCAATTGTAAAAGACTGCAAAGAGGCTGACTTATCC; TTGTATAATGAATTCCGATTGTGGAAGGATGAGCCCACAATGGACAGGACGTGTCCTTTC; TTAGACAAAATCTACCAGGAAGATATCTTTCCATGTTTAACATTCTCAAAAAGTGAGTTG; GCTTCAGCTGTTCTGGAGGCTGTGGAAAACAATACTCTAAGCATTGAACCAGTGGGATTA; CAACCTATCCGGTTTGTGAAAGCTTCTGCAGTTGAATGCGGAGGACCAAAAAAATGTGCT; CTCACTGGCCAGAGTAAGTCCTGTAAACACAGAATTAAATTAGGGGACTCAAGCAACTAT; TATTATATTTCTCCTTTTTGCAGATACAGGATCACTTCTGTATGTAACTTTTTTACATAC; ATTCGATACATTCAGCAGGGACTCGTGAAACAGCAGGATGTTGATCAGATGTTTTGGGAG; GTTATGCAGTTGAGAAAAGAGATGTCATTGGCAAAGCTGGGTTATTTCAAAGAGGAACTC; TGA; ; Clone variation with respect to NM_022456.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022456 unedited; TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTC; GGCACGAGGGCGTAGTGAGCCCGCCGCCGTGGAGTGTAGCGGAGAGGGCTCGCCGTCCTC; CTCCGTTTCTCGCTGCTTCGGGACGCGCTCTCTGCGGCTCTGTGAGCGCCCCTGAGCGCC; GGCAGCGGCCGCGGTGGGTTCTTCAGGTTATCTTATGATGAGGCTTTTGCTATGGCTAAT; GATCCCTTGGAAGGCTTCCATGAAGTAAACCTTGCTTCACCTACTTCTCCGGACCTTCTT; GGTGTGTATGAATCAGGAACTCAAGAGCAGACTACCTCACCAAGTGTCATCTACCGGCCA; CACCCTTCAGCTTTATCCTCTGTACCTATCCAGGCAAATGCATTAGATGTTTCTGAACTT; CCTACACAACCCGTGTATTCATCCCCCAGACGTTTAAATTGTGCGGAAATATCTAGTATC; AGCTTTCATGTTACAGACCCAGCCCCTTGCTCTACCTCTGGAGTCACAGCTGGATTAACT; AAATTAACTACAAGAAAGGACAACTATAATGCAGAGAGAGAGTTTTTACAGGGTGCTACT; ATAACAGAGGCTTGCGATGGCAGTGATGATATTTTTGGGTTGAGTACTGATAGTCTGTCT; CGTTTACGAAGCCCATCTGTTTTGGAAGTTAGAGAAAAGGGCTATGAACGATTAAAAGAA; GAACTCGCANAAGCTCAGAGGGAACTGAAGTTAAAGATGAAGATGTGAGNAGGCTTCAAA; GTGCGAGNATCACTTGGACAGGGATTGGAGAACTACAGCTAGTCTATTGAGAAGCTCAAA; NATGGTGAGAGAGCAATATCAGCAGGGCACAGCGAAAACAGCTAAGAGCCAGGGAAATT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022456 unedited; ACGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTCAATCTAACATTTATTTATTTCAG; GTTTTTTTTTTTTTTGGCAACTATAAATTACATTCACTAGTACACCTGGAATTCTTCATA; GTGTGTTCAACTAAAGAATAAGCAAACACAGCATAGATCAGTTTAAAAGAAATCATGGTA; CTTCTTAAAGCAATTTGCAAAAGGGGAAAAAAGTCTTAGGCTCACTCCTTGGAAATAAAT; ATCAAGTAACCATAAAAATATTCAGCCATTTTTCAGTTATTCGGGGAGTTCAGGCATGGT; CCCACGCAAAGCATCAAAGTTCCTCTTTGAAATAACCCAGCTTTGCCAATGACATCTCTT; TTCTCAACTGCATAACCTCCCAAAACATCTGATCAACATCCTGCTGTTTCACGAGTCCCT; GCTGAATGTATCGAATGTATGTAAAAAAGTTACATACAGAAGTGATCCTGTATCTGCAAA; AAGGAGAAATATAATAATAGTTGCTTGAGTCCCCTAATTTAATTCTGTGTTTACAGGACT; TACTCTGGCCAGTGAGAGCACATTTTTTTGGTCCTCCGCATTCAACTGCAGAAGCTTTCA; CAAACCGGATAGGTTGTAATCCCACTGGTTCAATGCTTAAAGTATTGTTTTCCACAGCCT; CCAGAACAGCTGAAGCCAACTCACTTTTTGAGAATGTTAAACATGGAAAGATATCTTCCT; GGTAGATTTTGTCTAAGAAGGACACGTNCTGTCCATTGTGGGCTCATCCCTTTCCACATC; GGATTCATTATACAGGGGATAGTCAGCCTCTTTGCAGTCTTTTACAATTGGCTGTATCAC; ACTGAGTCCTGATGACTGCCACTCATAGCACTGCTTGAGCTTTTATTTCTGGNATGCACC; TTTTAAAAGGTNGTCTTNCACTGCAAAGGCTCTGCGTAGTGATGTGGAAACTGACATACA; GGTCTCATGCACTACTAGCTGAGACACAATTTNCCTGAGCTCTTTACAGTTTCTGCGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_022456
• Insert Size: 1840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022456.2, NP_071901.2
• RefSeq Size: 1855 bp
• RefSeq ORF: 1383 bp
• Locus ID: 117177
• UniProt ID: Q96QF0
• Cytogenetics: 12q15
• Summary: Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (alpha 1) has a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant alpha 2. The resulting isoform (alpha 1) has a shorter N-terminus, compared to isoform alpha 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.