SNURF (NM_022804) Human Untagged Clone

SKU SC112432

SNURF (untagged)-Human SNRPN upstream reading frame (SNURF), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: SNURF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112432 sequence for NM_022804 edited (data generated by NextGen Sequencing); ATGGAGCGGGCAAGGGATCGCTTACACCTGAGACGAACTACAGAACAGCACGTACCAGAG; GTGGAAGTCCAAGTCAAACGCAGAAGGACTGCCTCACTGAGCAACCAAGAGTGTCAGTTG; TACCCGAGGCGTTCTCAGCAGCAGCAAGTACCTGTGGTGGATTTCCAGGCTGAACTGAGG; CAGGCATTCTTAGCTGAGACACCAAGAGGTGGTTAA; ; Clone variation with respect to NM_022804.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022804 unedited; TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGATGCCTGACGCATCTGT; CTGAGGAGCGGTCAGTGACGCGATGGAGCGGGCAAGGGATCGCTTACACCTGAGACGAAC; TACAGAACAGCACGTACCAGAGGTGGAAGTCCAAGTCAAACGCAGAAGGACTGCCTCACT; GAGCAACCAAGAGTGTCAGTTGTACCCGAGGCGTTCTCAGCAGCAGCAAGTACCTGTGGT; GGATTTCCAGGCTGAACTGAGGCAGGCATTCTTAGCTGAGACACCAAGAGGTGGTTAAAG; CCATATTGGAGTAGCGAGGAATCTGATTCCAAGCAAAAACCAGGCTCCATCTACTCTTTG; AAGCTTCTGCCCAGCTTGCATTGTTTCTAGGAGAACCTGCGTCATACCTTTATCTATAGC; CTTCCCCTAGGTCTTCAGAAGCATCAAGTTTTAACTGTGGACATTGGATTTGGTGGAACA; GCAATCATGACTGTTGGCAAGAGTAGCAAGATGCTGCAGCACATTGACTATAGAATGAGA; TGTATCCTGCAAGATGGCCGAATCTTCATTGGCACCTTTAAGGCTTTTGACAAGCATATG; AATTTGATCCTCTGTGATTGTGATGAGTTCAGAAAGATCAAGCCAAAGAATGCGAAGCAA; CCAGAGCGTGAAAAAAGCGGGTTTTGGGGTCTGGGTGGTGCTGCGTGGGGAGAACTTGGT; ATCCATGACTGTGNGAGGNGCCCACCCCCCANAGATACTGGCATTGCTCGGNNTACCCAC; TTGCTGAGCTGCTGGNAGCCCCTGNNNGGTTGTAGGGCANCTGTAAAAGGATACCANCTG; GTGTGCCATTCCCCAGCCCCCTGCTGATNGNCAGCCCTGTCCAAGGGATTGGGGGACATN; CCANCAGTATGACTCCAGGNA;
• Restriction Sites: NotI-NotI
• ACCN: NM_022804
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022804.1, NP_073715.1
• RefSeq Size: 347 bp
• RefSeq ORF: 216 bp
• Locus ID: 8926
• UniProt ID: Q9Y675
• Cytogenetics: 15q11.2
• Protein Families: Stem cell - Pluripotency
• Summary: This gene is located within the Prader-Willi Syndrome critical region on chromosome 15. Transcripts produced from this gene initiate at an imprinting center and are paternally-imprinted. These transcripts may be bicistronic and also encode SNRPN (small nuclear ribonucleoprotein polypeptide N) from a downstream open reading frame. The small protein represented by this gene is encoded by an evolutionarily-conserved upstream open reading frame and is localized to the nucleus. Extensive alternative splicing and promoter usage occurs in this region and the full-length nature of some of these transcripts has not been determined. Alterations in the imprinting center are associated with parental imprint switch failure, which may cause Angelman syndrome or Prader-Willi syndrome. [provided by RefSeq, Mar 2017]; Transcript Variant: This variant (2) lacks multiple 3' exons and contains an alternate 3' UTR compared to variant 1. This variant is monocistronic and cannot encode the SNRPN protein.