NEIL1 (NM_024608) Human Untagged Clone

SKU SC112118

NEIL1 (untagged)-Human nei endonuclease VIII-like 1 (E. coli) (NEIL1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: NEIL1
• Synonyms: FPG1; hFPG1; NEI1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024608, the custom clone sequence may differ by one or more nucleotides; ; ; ATGCCTGAGGGCCCCGAGCTGCACCTGGCCAGCCAGTTTGTGAATGAGGCCTGCAGGGCGCTGGTGTTCG; GCGGCTGCGTGGAGAAGTCCTCTGTCAGCCGCAACCCTGAGGTGCCCTTTGAGAGCAGTGCCTACCGCAT; CTCAGCTTCAGCCCGCGGCAAGGAGCTGCGCCTGATACTGAGCCCTCTGCCTGGGGCCCAGCCCCAACAG; GAGCCACTGGCCCTGGTCTTCCGCTTCGGCATGTCCGGCTCTTTTCAGCTGGTGCCCCGCGAGGAGCTGC; CACGCCATGCCCACCTGCGCTTTTACACGGCCCCGCCTGGCCCCCGGCTCGCCCTATGTTTCGTGGACAT; CCGCCGGTTCGGCCGCTGGGACCTTGGGGGAAAGTGGCAGCCGGGCCGCGGGCCCTGTGTCTTGCAGGAG; TACCAGCAGTTCAGGGAGAATGTGCTACGAAACCTAGCGGATAAGGCCTTTGACCGGCCCATCTGCGAGG; CCCTCCTGGACCAGAGGTTCTTCAATGGCATTGGCAACTATCTGCGGGCAGAGATCCTGTACCGGCTGAA; GATCCCCCCCTTTGAGAAGGCCCGCTCGGTCCTGGAGGCCCTGCAGCAGCACAGGCCGAGCCCGGAGCTG; ACCCTGAGCCAGAAGATAAGGACCAAGCTGCAGAATCCAGACCTGCTGGAGCTATGTCACTCAGTGCCCA; AGGAAGTGGTCCAGTTGGGGGGCAAAGGCTACGGGTCAGAGAGCGGGGAGGAGGACTTTGCTGCCTTTCG; AGCCTGGCTGCGCTGCTATGGCATGCCAGGCATGAGCTCCCTGCAGGACCGGCATGGCCGTACCATCTGG; TTCCAGGGGGATCCTGGACCGTTGGCACCCAAAGGGCGCAAGTCCCGCAAAAAGAAATCCAAGGCCACAC; AGCTGAGTCCTGAGGACAGAGTGGAGGACGCTTTGCCTCCAAGCAAGGCCCCTTCCAGGACACGAAGGGC; AAAGAGAGACCTTCCTAAGAGGACTGCAACCCAGCGGCCTGAGGGGACCAGCCTCCAGCAGGACCCAGAA; GCTCCCACAGTGCCCAAGAAGGGGAGGAGGAAGGGGCGACAGGCAGCCTCTGGCCACTGCAGACCCCGGA; AGGTCAAGGCTGACATCCCATCCTTGGAACCAGAGGGGACCTCAGCCTCTTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024608 unedited; CCTTGCATTTGTATCCGATTTATATAGGCGGCCCGATTCGCCGAGAACGAACCATCTCAG; TGGGTCTACCTCCTCGCCTTTTTTTGTTGTTGTTGTTGCTTGGCTGCGCTTCTGACAGGG; CAGGCCGTGATGATGTTTGTTTATGAGTTAGGTCTGACTGTTCGTTGGTGCTTAAGATCC; CCACCGGGTCCCTAGGGCCTGTGCTTTCCGCGCACCTGTGCACGTCCTGCGCGCAGCTGC; AGGCGACTCCGCTCTGGCTCGTCGCTGCTGTTTCCTGCTGGGGGTGCCGACCCTGTCCCA; CGCTAGCTGGGTGACTTCCCCCAACCGCAGAGACAGCGGCGACCCGGGGCCTCAGACCTG; CCCCCGCATCTCGCCGGCGCCAGGCAGTGGGAAGTCAGGTTCTTCCGCCACCTCCCAGCC; AGGACTCTGCCACCCTCCCTCAGGATGCCTGAGGGCCCCGAGCTGCACCTGGCCAGCCAG; TTTGTGAATGAGGCCTGCAGGGCGCTGGTGTTCGGCGGCTGCGTGGAGAAGTCCTCTGTC; AGCCGCAACCCTGAGGTGCCCTTTGAGAGCAGTGCCTACCGCATCTCAGCTTCAGCCCGC; GGCAAGGAGCTGCGCCTGATACTGAGCCCTCTGCCTGGGGCCCAGCCCCAACAGGAGCCA; CTGGCCCTGGTCTTCCGCTTCGGCATGTCCGGCTCTTTTCAGCTGGTGCCCCGCGAGGAG; CTGCCACGCCATGCCCACCTGCGCTTTTACACNGCCCCGCCTGGCCCCCAGCTCGCCCTA; TGTTTCGTGGACATCCGCCGGTTCGGCCGCTGGGACCTTGGGGGAAAGTGCAGCCGGGCC; GCGGGCCCTGTGTCTTTGCAGAGTACCAGCAGTTCAGGGAGAATGTGCTACGAAACCTAG; CGGATAAGGCCTTTGACCGGCCCATCTGCGAGGCCCTNCTGGACCAGNAGTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_024608
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_024608.1, NP_078884.1
• RefSeq Size: 1828 bp
• RefSeq ORF: 1173 bp
• Locus ID: 79661
• UniProt ID: Q96FI4
• Cytogenetics: 15q24.2
• Domains: Fapy_DNA_glyco
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Summary: This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]; Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.