FBXO31 (NM_024735) Human Untagged Clone

SKU SC112027

FBXO31 (untagged)-Human F-box protein 31 (FBXO31)

Product Data

• Type: Human Untagged Clone
• Target Symbol: FBXO31
• Synonyms: FBX14; Fbx31; FBXO14; MRT45; pp2386
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024735, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGGTGTGTGCTCGCCTTTGCGGCGTGGGCCCGTCGCGCGGATGTCGGCGCCGCCAGCAGCGCCGGG; GCCCGGCCGAGACGGCGGCGGCCGACAGCGAGCCGGACACAGACCCCGAGGAGGAGCGCATCGAGGCTAG; CGCCGGGGTCGGGGGCGGCTTGTGCGCGGGCCCCTCGCCGCCGCCCCCGCGCTGCTCGCTGCTGGAGCTG; CCGCCCGAGCTGCTGGTGGAGATCTTCGCGTCGCTGCCGGGCACGGACCTACCCAGCTTGGCCCAGGTCT; GCACGAAGTTCCGGCGCATCCTCCACACCGACACCATCTGGAGGAGGCGTTGCCGTGAGGAGTATGGTGT; TTGCGAAAACTTGCGGAAGCTGGAGATCACAGGCGTGTCTTGTCGGGACGTCTATGCGAAGCTGCTTCAC; CGATATAGACACATTTTGGGATTGTGGCAGCCAGATATCGGGCCATACGGAGGACTGCTGAACGTGGTGG; TGGACGGCCTGTTCATCATCGGGTGGATGTACCTGCCTCCCCATGACCCCCACGTCGATGACCCTATGAG; ATTCAAGCCTCTGTTCAGGATCCACCTGATGGAGAGGAAGGCTGCCACAGTGGAGTGCATGTACGGCCAC; AAAGGGCCCCACCACGGCCACATCCAGATTGTGAAGAAGGATGAGTTCTCCACCAAGTGCAACCAGACGG; ACCACCACAGGATGTCCGGCGGGAGGCAGGAGGAGTTTCGGACGTGGCTGAGGGAGGAATGGGGGCGCAC; GCTGGAGGACATCTTCCACGAGCACATGCAGGAGCTCATCCTGATGAAGTTCATCTACACCAGTCAGTAC; GACAACTGCCTGACCTACCGCCGCATCTACCTGCCGCCCAGCCGCCCCGACGACCTCATCAAGCCTGGCC; TCTTCAAAGGTACCTATGGCAGCCACGGCCTGGAGATTGTGATGCTCAGCTTCCACGGCCGGCGTGCCAG; GGGCACCAAGATCACGGGCGACCCCAACATCCCCGCTGGGCAGCAGACAGTGGAGATCGACCTGAGGCAT; CGGATCCAGCTGCCCGACCTCGAGAACCAGCGCAACTTCAATGAGCTCTCCCGCATCGTCCTGGAGGTGC; GCGAGAGGGTGCGCCAGGAGCAGCAGGAAGGCGGGCACGAGGCGGGCGAGGGTCGTGGCCGGCAGGGCCC; CCGGGAGTCCCAGCCAAGCCCTGCCCAGCCCAGGGCAGAGGCGCCCAGCAAGGGCCCAGATGGGACACCT; GGTGAGGATGGTGGCGAGCCTGGGGATGCCGTAGCTGCGGCCGAGCAGCCTGCCCAGTGTGGGCAGGGGC; AGCCGTTCGTGCTGCCCGTGGGCGTGAGCTCCAGGAATGAGGACTACCCCCGAACCTGCAGGATGTGTTT; TTATGGCACAGGCCTCATCGCGGGCCACGGCTTCACCAGCCCTGAACGCACCCCCGGGGTCTTCATCCTC; TTCGATGAGGACCGCTTCGGGTTCGTCTGGCTGGAGCTGAAATCCTTCAGCCTGTACAGCCGGGTCCAGG; CCACCTTCCGGAACGCAGATGCGCCGTCCCCACAGGCCTTCGATGAGATGCTCAAGAACATTCAGTCCCT; CACCTCCTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024735 unedited; ATTTTCCCCGCCCCGTTGNCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA; GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG; CGAATTCGGCACGAGGGCGGCATGGCGGTGTGTGCTCGCCTTTGCGGCGTGGGCCCGTCG; CGCGGATGTCGGCGCCGCCAGCAGCGCCGGGGCCCGGCCGAGACGGCGGCGGCCGACAGC; GAGCCGGACACAGACCCCGAGGAGGAGCGCATCGAGGCTAGCGCCGGGGTCGGGGGCGGC; TTGTGCGCGGGCCCCTCGCCGCCGCCCCCGCGCTGCTCGCTGCTGGAGCTGCCGCCCGCT; CTGCTGGTGGAGATCTTCGCGTCGCTGCCGGGCACGGACCTACCCAGCTTGGCCCAGGTC; TGCACGAAGTTCCGGCGCATCCTCCACACCGACACCATCTGGAGGAGGCGTTGCCGTGAG; GAGTATGGTGTTTGCGAAAACTTGCGGAAGCTGGAGATCACAGGCGTGTCTTGTCGGGAC; GTCTATGCGAAGCTGCTTTACCGATATAGACACATTTTGGGATTGTGGCAGCCAGATATC; GGGCCATACGGAGGACTGCTGAACGTGGCGGGTGGACGGCCCTGCTCTCATCCGGCTGGA; CGTACCTGCCTCCCCATGACCCCCCAGGTCGATGACCCCTATGAGACTCAAGTCCTTTGG; TCCAGGACCCCCCTCGATGGCCAAGCACGGCTGCCCCCGTTGGCCTGCCATGTACCGGCA; AAAAGGGCCCCTACAACGGGCCAAATTCAAATTGTGAAAAAAGGCCCAGTCCCCTCCACC; ACCCGTCATCCCGACTGTACCCCTCCAGCATTTCCGCGCCCTATGCCCGTAAGTACCTCC; ACCACCCCCGTCTCTACGAAGTAACGGCCCCCTCCTNCTCCTCTTTCCTTCCCCCTCCCC; CTTTCCGTTCTCTCCCCCTTCAATCCCCCCG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024735 unedited; NNNCCGGTCTAGCTATGNNACCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTGAGATCTGAGATTCCTTTAATCAGAAGCACGTGCGTCCCA; CAGTGTGCTCTTCAAGCCCCAAAGGGCACGCCTCTAGGACTGCGTCCCTTAAAGCGAGGC; TCGGGATCTTGGTAAAAAAGCATTTGCTTGATTTTATTTAAACAATGGTGAATCTTCAAG; GGGCCAGTCTACATGCCCAACAGTCCTCCAGGCTTTAAGGCCACAGTCACCGTCACTCAA; AGACTGCCTCATTTGGCAAGAGAGAAAAACAGTGACCACCCCAGAGGGCAGGGGGTGACA; AAACTTGTAGGCTAATGCTGCAAAAGCCCCTTGAAACTGGGGCCCCACACAAAAACCAGC; AGGGGCGCCTCGGGGTCTGCGTGGGTCCCCCTGCTGCCCCCCTGGAAAGGCGCTTTTTCC; ACTGGGTTTTTTTGTGAGTCTTTCTTCCCGGCCGGCCCGATTACCCTTTAGGAACCCTTG; CCAATTTTCTTGATCTGGGAAACAAAATAAGCATTTGCCCAAAGCACTGGGGCTGGGCCA; TGGGAATTCTTTTTGTCTTTTAAGGCCCCTCTAGGGGAAATTTTTTCGGGGAAAATTTTT; CCCGGACCATCCCTCAGGGTTGGGCTCCCAACCCAAGGACACAAGACTGTCCACCTTCCC; GAGGGGCCTTTCCACCAGGACCTGTCCGGGGGGCCCACCCAAGGGCTTGGGCACCCCCAC; CCAAGGCCAACCGCCTAAAGGGAAGGTCCTTGGGACACCCCTCCTGTACCGACTTTCTTT; GGCTTGGGGAAAGACCACTTTGGACCACGCTTTTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_024735
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_024735.2, NP_079011.3
• RefSeq Size: 3635 bp
• RefSeq ORF: 1620 bp
• Locus ID: 79791
• UniProt ID: Q5XUX0
• Cytogenetics: 16q24.2
• Domains: F-box
• Protein Families: Druggable Genome
• Summary: This gene is a member of the F-box family. Members are classified into three classes according to the substrate interaction domain, FBW for WD40 repeats, FBL for leucing-rich repeats, and FBXO for other domains. This protein, classified into the last category because of the lack of a recognizable substrate binding domain, has been proposed to be a component of the SCF ubiquitination complex. It is thought to bind and recruit substrate for ubiquitination and degradation. This protein may have a role in regulating the cell cycle as well as dendrite growth and neuronal migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).