KEAP1 (NM_012289) Human Untagged Clone

SKU SC111946

KEAP1 (untagged)-Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: KEAP1
• Synonyms: INrf2; KLHL19
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_012289, the custom clone sequence may differ by one or more nucleotides; ; ; ATGCAGCCAGATCCCAGGCCTAGCGGGGCTGGGGCCTGCTGCCGATTCCTGCCCCTGCAGTCACAGTGCC; CTGAGGGGGCAGGGGACGCGGTGATGTACGCCTCCACTGAGTGCAAGGCGGAGGTGACGCCCTCCCAGCA; TGGCAACCGCACCTTCAGCTACACCCTGGAGGATCATACCAAGCAGGCCTTTGGCATCATGAACGAGCTG; CGGCTCAGCCAGCAGCTGTGTGACGTCACACTGCAGGTCAAGTACCAGGATGCACCGGCCGCCCAGTTCA; TGGCCCACAAGGTGGTGCTGGCCTCATCCAGCCCTGTCTTCAAGGCCATGTTCACCAACGGGCTGCGGGA; GCAGGGCATGGAGGTGGTGTCCATTGAGGGTATCCACCCCAAGGTCATGGAGCGCCTCATTGAATTCGCC; TACACGGCCTCCATCTCCATGGGCGAGAAGTGTGTCCTCCACGTCATGAACGGTGCTGTCATGTACCAGA; TCGACAGCGTTGTCCGTGCCTGCAGTGACTTCCTGGTGCAGCAGCTGGACCCCAGCAATGCCATCGGCAT; CGCCAACTTCGCTGAGCAGATTGGCTGTGTGGAGTTGCACCAGCGTGCCCGGGAGTACATCTACATGCAT; TTTGGGGAGGTGGCCAAGCAAGAGGAGTTCTTCAACCTGTCCCACTGCCAACTGGTGACCCTCATCAGCC; GGGACGACCTGAACGTGCGCTGCGAGTCCGAGGTCTTCCACGCCTGCATCAACTGGGTCAAGTACGACTG; CGAACAGCGACGGTTCTACGTCCAGGCGCTGCTGCGGGCCGTGCGCTGCCACTCGTTGACGCCGAACTTC; CTGCAGATGCAGCTGCAGAAGTGCGAGATCCTGCAGTCCGACTCCCGCTGCAAGGACTACCTGGTCAAGA; TCTTCGAGGAGCTCACCCTGCACAAGCCCACGCAGGTGATGCCCTGCCGGGCGCCCAAGGTGGGCCGCCT; GATCTACACCGCGGGCGGCTACTTCCGACAGTCGCTCAGCTACCTGGAGGCTTACAACCCCAGTGACGGC; ACCTGGCTCCGGTTGGCGGACCTGCAGGTGCCGCGGAGCGGCCTGGCCGGCTGCGTGGTGGGCGGGCTGT; TGTACGCCGTGGGCGGCAGGAACAACTCGCCCGACGGCAACACCGACTCCAGCGCCCTGGACTGTTACAA; CCCCATGACCAATCAGTGGTCGCCCTGCGCCCCCATGAGCGTGCCCCGTAACCGCATCGGGGTGGGGGTC; ATCGATGGCCACATCTATGCCGTCGGCGGCTCCCACGGCTGCATCCACCACAACAGTGTGGAGAGGTATG; AGCCAGAGCGGGATGAGTGGCACTTGGTGGCCCCAATGCTGACACGAAGGATCGGGGTGGGCGTGGCTGT; CCTCAATCGTCTCCTTTATGCCGTGGGGGGCTTTGACGGGACAAACCGCCTTAATTCAGCTGAGTGTTAC; TACCCAGAGAGGAACGAGTGGCGAATGATCACAGCAATGAACACCATCCGAAGCGGGGCAGGCGTCTGCG; TCCTGCACAACTGTATCTATGCTGCTGGGGGCTATGATGGTCAGGACCAGCTGAACAGCGTGGAGCGCTA; CGATGTGGAAACAGAGACGTGGACTTTCGTAGCCCCCATGAAGCACCGGCGAAGTGCCCTGGGGATCACT; GTCCACCAGGGGAGAATCTACGTCCTTGGAGGCTATGATGGTCACACGTTCCTGGACAGTGTGGAGTGTT; ACGACCCAGATACAGACACCTGGAGCGAGGTGACCCGAATGACATCGGGCCGGAGTGGGGTGGGCGTGGC; TGTCACCATGGAGCCCTGCCGGAAGCAGATTGACCAGCAGAACTGTACCTGTTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012289 unedited; TCACTATATTTGTATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGAGCGC; GGCCATGGCGGGGTCCCTGAGTGCCAGAGGTGGTGGTGTTGCTTATCTTCTGGAACCCCA; TGCAGCCAGATCCCAGGCCTAGCGGGGCTGGGGCCTGCTGCCGATTCCTGCCCCTGCAGT; CACAGTGCCCTGAGGGGGCAGGGGACGCGGTGATGTACGCCTCCACTGAGTGCAAGGCGG; AGGTGACGCCCTCCCAGCATGGCAACCGCACCTTCAGCTACACCCTGGAGGATCATACCA; AGCAGGCCTTTGGCATCATGAACGAGCTGCGGCTCAGCCAGCAGCTGTGTGACGTCACAC; TGCAGGTCAAGTACCAGGATGCACCGGCCGCCCAGTTCATGGCCCACAAGGTGGTGCTGG; CCTCATCCAGCCCTGTCTTCAAGGCCATGTTCACCAACGGGCTGCGGGAGCAGGGCATGG; AGGTGGTGTCCATTGAGGGTATCCACCCCAAGGTCATGGAGCGCCTCATTGAATTCGCCT; ACACGGCCTCCATCTCCATGGGCGAGAAGTGTGTCCTCCACGTCATGAACGGTGCTGTCA; TGTACCAGATCGACAGCGTTGTCCGTGCCTGCAGTGACTTCCTGGTGCAGCAGCTGGACC; CCAGCAATGCCATCGGCATCGCCCACTTCGCTGAGCAGATTGGCTGTGTGGAGTTGCACC; ACCGTGCCCGGGGAGTACATCTACATGCATTTTGGGGAGGTTGGCCAACCAAGAGGAGTT; CTTCACCCTGTCCCACTGGCCACTGGGTGACCCTCATCAGCCGGGACGACCTGNACGTGC; GCTGCGAGTCCCAAGTCTTCCACGCCCTGCATCAACTGGGTGCAAGNACGACTGCGAACA; GCGACCGTTTTACNTTCAGGCGCTTGCTGCGGGCCCGGCG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012289 unedited; GCACGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAT; TAGTCTGTTCTTTATTTTCCTTGGAAAATTACAGGGTTACTTATCAACAAAATAACTGTC; CCTCCGGGGGGGAAGTGCCATCCGGTGGCTTCTATGTACAGGCCCATCCCTGGGAACCAC; ATTTCCAAAGAGGGGCCTCCCCCTGAGGCCCCCATTGGCCCCCTCCCAGGTATCCAAGAA; TAAATCACATGGTGACAGCTGCCGGCATGGGTGAGTGGGACCCAAGCCTATTACCCGGCC; AGAGGGTTTGGGGGCCTCTCTCCTGGAAGCCTGCTCTTTCCACACCCCCTTTCCCAGCCA; GGCTGTCTTGGACACTCCCGGGGCTCCGCTGAGGGGCACATGATTCCCGCTTTGGACTTC; TTTTGAGATGTCATTTTAACACTGAGGCATCCTGGCCTCCCTTCCCGGAAAATGGGTTAT; TTGCAGAGCTGGCTTTTCTTTTAATCCCGGTTTTTGTACAAAAACAATGATACTCCCCAT; TGGACTGTATTTTTGCCCAAAAAACAAAAGTGCCTCAACAGGTACAGTTCTGCTGGGCAA; TCTGCTTCCCGGAGGGCTCCATGGTGACAGCCACCCCCACCCCACTCCCGCCCCGATGGC; TTTCCGGTCACCTCGCCCCAAGTGGCTTGATTCTGGGCCGAACCCTCCACACTGGTCCGG; AACCTGTGACCCTCTTAGCCCCAAGGCCTAAATTCTCCCCTGGGGCCAAGGATCCCCAGG; CCCTTTCCGGGCCTTTGGGGCCTCCAAACTCCCGTCTCTTTTCACCACAAACGCCCCCCC; TGTCACCGGGCCCGCCCCCATCCCCCCCCCCTAATACCCCCGGACAGACCAAACCCCTCC; CCTTCCGGAGGGCCCCTGCTCTACCCCCGCACCCCCCCTTTGGGGTAGAACCCCACGAAT; ACGCCGTCGCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_012289
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012289.3, NP_036421.2
• RefSeq Size: 2577 bp
• RefSeq ORF: 1875 bp
• Locus ID: 9817
• UniProt ID: Q14145
• Cytogenetics: 19p13.2
• Domains: BTB, Kelch
• Protein Families: Transcription Factors
• Protein Pathways: Ubiquitin mediated proteolysis
• Summary: This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) has an alternate 5' UTR, as compared to variant 1.