EXOC2 (NM_018303) Human Untagged Clone

CAT#: SC111916

EXOC2 (untagged)-Human exocyst complex component 2 (EXOC2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EXOC2
• Synonyms: NEDFACH; SEC5; SEC5L1; Sec5p
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111916 sequence for NM_018303 edited (data generated by NextGen Sequencing)
  ATGTCTCGATCACGACAACCCCCCCTTGTGACCGGCATCTCTCCAAATGAAGGGATACCA
  TGGACGAAGGTCACAATCAGGGGAGAAAATCTGGGGACTGGCCCCACCGACCTCATAGGC
  TTGACCATTTGTGGACATAATTGCCTCCTGACGGCAGAATGGATGTCTGCAAGTAAAATA
  GTATGTCGAGTGGGACAAGCCAAAAATGACAAAGGAGACATTATTGTCACCACTAAGTCA
  GGTGGCAGAGGAACCTCAACAGTCTCTTTCAAGCTACTCAAACCTGAGAAAATAGGCATT
  TTGGATCAGTCTGCTGTGTGGGTTGATGAAATGAATTATTATGATATGCGCACTGACAGG
  AACAAAGGAATTCCGCCCTTGTCCTTACGTCCTGCTAACCCGCTTGGCATTGAGATTGAA
  AAAAGTAAATTTTCGCAGAAGGACTTAGAAATGCTATTCCATGGAATGAGTGCTGATTTT
  ACAAGTGAGAATTTCTCAGCAGCCTGGTATCTTATAGAGAATCACTCAAACACCAGTTTT
  GAGCAGCTCAAAATGGCAGTCACCAACCTAAAGAGACAGGCTAACAAGAAGAGTGAGGGC
  AGCCTGGCCTATGTGAAAGGCGGTCTCAGTACATTCTTCGAAGCACAGGATGCCCTCTCA
  GCCATCCATCAAAAACTAGAAGCAGATGGAACGGAAAAAGTAGAAGGATCCATGACGCAG
  AAACTGGAGAATGTTCTGAACAGAGCAAGTAATACTGCAGACACATTGTTTCAAGAAGTA
  TTAGGTCGGAAAGACAAGGCAGATTCCACTAGAAATGCACTCAATGTGCTTCAGCGATTT
  AAGTTTCTTTTCAACCTTCCTCTAAATATTGAAAGGAATATTCAAAAGGGTGATTATGAT
  GTGGTTATTAATGATTATGAAAAGGCCAAGTCACTTTTTGGGAAAACGGAGGTGCAAGTT
  TTCAAGAAATATTATGCTGAAGTAGAAACAAGGATTGAAGCTTTAAGAGAATTACTTCTG
  GATAAATTGCTTGAGACACCATCAACTTTACATGACCAAAAACGTTACATAAGGTACCTG
  TCTGACCTTCATGCGTCTGGTGACCCTGCTTGGCAATGCATTGGAGCCCAACACAAGTGG
  ATCCTTCAGCTCATGCACAGTTGCAAAGAGGGCTACGTGAAAGATCTGAAAGGTAACCCA
  GGCCTGCACAGTCCCATGTTGGATCTTGATAATGATACACGTCCCTCAGTGTTGGGCCAT
  CTCAGTCAGACAGCGTCCCTGAAGAGGGGCAGCAGCTTTCAGTCTGGTCGAGACGACACG
  TGGAGATACAAAACTCCCCACAGGGTGGCCTTTGTTGAAAAATTGACAAAACTCGTCTTG
  AGCCAGCTGCCTAACTTCTGGAAACTCTGGATCTCCTACGTTAATGGAAGCCTCTTCAGT
  GAGACTGCTGAGAAGTCAGGCCAGATTGAAAGATCAAAGAATGTAAGGCAAAGACAAAAT
  GATTTTAAGAAAATGATTCAGGAAGTAATGCACTCCCTGGTGAAGCTTACCCGCGGAGCC
  CTGCTTCCCCTCAGCATCCGGGATGGGGAAGCCAAGCAGTACGGAGGCTGGGAGGTGAAG
  TGCGAGCTCTCCGGACAGTGGCTCGCTCACGCCATCCAGACTGTAAGACTTACTCACGAA
  TCGTTGACTGCCCTTGAAATTCCTAATGACCTGTTACAGACTATCCAGGATCTCATCTTG
  GATCTCCGAGTACGTTGCGTAATGGCCACGTTGCAGCACACGGCGGAAGAAATAAAGAGA
  TTAGCTGAAAAAGAAGACTGGATTGTTGACAATGAAGGACTGACTTCTCTACCATGTCAG
  TTTGAACAGTGCATCGTGTGTTCTCTGCAGTCACTGAAGGGGGTTCTGGAGTGCAAGCCG
  GGAGAGGCCAGTGTCTTCCAACAACCTAAAACACAGGAGGAGGTTTGCCAGCTAAGCATC
  AATATAATGCAGGTTTTTATATACTGTCTGGAACAGTTGAGCACCAAGCCTGATGCAGAT
  ATAGATACTACACATCTCTCTGTTGATGTTTCTTCCCCTGACTTGTTTGGAAGTATCCAT
  GAAGACTTCAGCTTGACCTCAGAACAGCGCCTTTTGATAGTCCTAAGTAATTGCTGCTAT
  CTAGAACGTCACACCTTCCTAAATATCGCAGAACATTTTGAAAAGCACAACTTCCAGGGA
  ATAGAAAAAATCACACAGGTTAGCATGGCCTCATTGAAAGAACTAGATCAAAGACTCTTT
  GAAAATTACATCGAGTTGAAAGCAGATCCCATCGTTGGCTCCTTAGAACCTGGAATTTAT
  GCAGGATATTTTGATTGGAAGGACTGCCTGCCTCCAACAGGTGTCAGAAACTATTTAAAA
  GAAGCACTGGTGAATATAATTGCCGTGCATGCAGAGGTGTTCACCATTTCCAAAGAACTG
  GTCCCTCGGGTACTATCCAAGGTGATAGAAGCAGTTTCTGAAGAGCTCAGTCGACTGATG
  CAGTGTGTTTCATCCTTCAGCAAAAATGGAGCTTTACAGGCGAGACTTGAAATCTGTGCT
  TTGAGGGACACTGTGGCTGTTTACCTGACACCCGAAAGCAAGTCAAGTTTTAAGCAGGCT
  TTGGAAGCCCTGCCCCAGCTTTCCAGTGGAGCAGATAAAAAGTTACTGGAAGAGCTCCTG
  AACAAGTTCAAGAGTAGCATGCACTTGCAGCTCACCTGTTTCCAAGCAGCTTCTTCAACC
  ATGATGAAAACATAA
  
  Clone variation with respect to NM_018303.4
  1677 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018303 unedited
  GATTATGTATCCGACTTACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGGCGAGACCT
  ACCGGGCCCTGTCTGTGCCGGCCGTGAGCTGCGGCAGGACGGCTGGAGATTACTTCTCTA
  ACAGGATCACCAGTTTGCTCCACAAAGCACAATGTCTCGATCACGACAACCCCCCCTTGT
  GACCGGCATCTCTCCAAATGAAGGGATACCATGGACGAAGGTCACAATCAGGGGAGAAAA
  TCTGGGGACTGGCCCCACCGACCTCATAGGCTTGACCATTTGTGGACATAATTGCCTCCT
  GACGGCAGAATGGATGTCTGCAAGTAAAATAGTATGTCGAGTGGGACAAGCCAAAAATGA
  CAAAGGAGACATTATTGTCACCACTAAGTCAGGTGGCAGAGGAACCTCAACAGTCTCTTT
  CAAGCTACTCAAACCTGAGAAAATAGGCATTTTGGATCAGTCTGCTGTGTGGGTTGATGA
  AATGAATTATTATGATATGCGCACTGACAGGAACAAAGGAATTCCGCCCTTGTCCTTACG
  TCCTGCTAACCCGCTTGGCATTGAGATTGAAAAAAGTAAATTTTCGCAGAAGGACTTAGA
  AATGCTATTCCATGGAATGAGTGCTGATTTTACAAGTGAGAATTTCTCAGCAGCCTGGTA
  TCTTATAGAGAATCACTCAAACACCAGTTNTGAGCAGCTCAAAATGGCAGTCACCAACCT
  AAAGAGACAGGCTAACAAGAAGAGTGAGGGCAGCCCTGCCTATGTGAAAGGCGGTCTCAG
  TACATTCTTCGAAGCACANGATGCCCTCTCAGCCATCCATCANANACTAGAAGCAGATGG
  AACGGNAAAAGTAGNAAGATCCATGACGCAGAAACTGGAGAATGTTCTGAACAGAGCAGT
  NATACTGCAGAACATTGNTTNCAGAAGTATTTAGTCGAAGACAGGCAGATNCACTAGAAT
  GCACTCATGTGCTCAGCGATTTAGCTTCTTTCAACCTTCTCTAATATTGAAGGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018303 unedited
  TATGGACCGGCGGCCGCAATCTAGNGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTAAAGGCAGATTCTTTATTGGCTGAATGGGCTGTAGTAGATATTT
  AAAATATCACAAACATTCATGATTGCAGTTTTTAAACTTAGCTCACAGTTGAGTTAGATA
  CCACAGTTAACATACTTAGGAAAGACAATTCTTAACAGCTTTATCAAGCACATTTGGGAA
  AGATTAAACTTTCATTTCCAGTTACCATGATGTCCCCCCATACCCCAAATGAACCAACCC
  ATTTTTTTATTTTAAATAATGGAAACTGTACATGGCTACCCTGGGCTCTAAAATTACTTG
  CATTAATGAGGACATTAATTTTAGCATTTAAAAAAAGAGATTTAAAAATAACCCTAGAAA
  AACTTGAAAACCCTGACAAGCCGACTACAAAGAGAATTCCTCTCTGAAAATTTTCAAATG
  CCACGGTATGAGGGAGTTGGGTGGGCTGCTGACTCTGAAAGAAGTCCCGGGGACCTGAAC
  CTATTCCAGTGAAGCCAGTCAGCAGTGAATGTGGGGCAAGTGCATGCGGCTGGTCTACAG
  CTCCCATTGCCTGGTGACCAAGGAGCATCCTGAAGTTTCAAAGAAAAAACTGTGTGACAG
  CAGCATTGTTCCAGCTACTGAGCAAGATCCTTCAGCAGGTAAGAAGTGGCAGAGACAGTC
  ACCTGCTGAAGGAGCGGGCAGGGAGTTGGCCGTCCCGCGGAGCCTGGCCTTTCCGTTAGG
  GGACCCTGAAGTCTGCGACCGCCCCGNCAACACCTGANAGTGTGACACAGTGCCCGTTAC
  CATTCACACTCCATCCAGATAACACACAGGCCCTCAGAACTGCACACGTGCATGTGTTGT
  GCGCCCGACAATTAGAGTGAGGGAGATGGAAGCTATGCCTTAACCTGTGAAAATAAAATC
  AGTAGCAAACGCAAGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018303
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018303.4, NP_060773.3
• RefSeq Size: 4458 bp
• RefSeq ORF: 2775 bp
• Locus ID: 55770
• UniProt ID: Q96KP1
• Cytogenetics: 6p25.3
• Domains: IPT
• Gene Summary: The protein encoded by this gene is a component of the exocyst complex, a multi-protein complex essential for the polarized targeting of exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and the functions of the exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. This interaction has been shown to mediate filopodia formation in fibroblasts. This protein has been shown to interact with the Ral subfamily of GTPases and thereby mediate exocytosis by tethering vesicles to the plasma membrane. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]
  Transcript Variant: This variant (1) represents the shorter, protein coding transcript. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.