TRIM5 alpha (TRIM5) (NM_033034) Human Untagged Clone

SKU SC111855

TRIM5 (untagged)-Human tripartite motif containing 5 (TRIM5), transcript variant alpha

Product Data

• Type: Human Untagged Clone
• Target Symbol: TRIM5 alpha
• Synonyms: RNF88; TRIM5alpha
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_033034 edited; GAATTCGGCACCAGCCAGGACAGGCAGGAGCAGTGGAATAGCTAGTATGGCTTCTGGAAT; CCTGGTTAATGTAAAGGAGGAGGTGACCTGCCCCATCTGCCTGGAACTCCTGACACAACC; CCTGAGCCTGGACTGCGGCCACAGCTTCTGCCAAGCATGCCTCACTGCAAACTACAAGAA; GTCCATGCTAGACAAAGGAGAGAGTAGCTGCCCTGTGTGCCGGATCAGTTACCAGCCTGA; GAACATACGGCCTAATCGGCATGTAGCCAACATAGTGGAGAAGCTCAGGGAGGTCAAGTT; GAGCCCAGAGGGGCAGAAAGTTGATCATTGTGCACGCCATGGAGAGAAACTTCTACTCTT; CTGTCAGGAGGACGGGAAGGTCATTTGCTGGCTTTGTGAGCGGTCTCAGGAGCACCGTGG; TCACCACACGTTCCTCACAGAGGAGGTTGCCCGGGAGTACCAAGTGAAGCTCCAGGCAGC; TCTGGAGATGCTGAGGCAGAAGCAGCAGGAAGCTGAAGAGTTAGAAGCTGACATCAGAGA; AGAGAAAGCTTCCTGGAAGACTCAAATACAGTATGACAAAACCAACGTCTTGGCAGATTT; TGAGCAACTGAGAGACATCCTGGACTGGGAGGAGAGCAATGAGCTGCAAAACCTGGAGAA; GGAGGAGGAAGACATTCTGAAAAGCCTTACGAACTCTGAAACTGAGATGGTGCAGCAGAC; CCAGTCCCTGAGAGAGCTCATCTCAGATCTGGAGCATCGGCTGCAGGGGTCAGTGATGGA; GCTGCTTCAGGGTGTGGATGGCGTCATAAAAAGAGCTGACAGATGTCCGACGCTACTGGG; TTGATGTGACAGTGGCTCCAAACAACATTTCATGTGCTGTCATTTCTGAAGATAAGAGAC; AAGTGAGCTCTCCGAAACCACAGATAATATATGGGGCACGAGGGACAAGATACCAGACAT; TTGTGAATTTCAATTATTGTACTGGCATCCTGGGCTCTCAAAGTATCACATCAGGGAAAC; ATTACTGGGAGGTAGACGTGTCCAAGAAAACTGCTTGGATCCTGGGGGTATGTGCTGGCT; TCCAACCTGATGCAATGTGTAATATTGAAAAAAATGAAAATTATCAACCTAAATACGGCT; ACTGGGTTATAGGGTTAGAGGAAGGAGTTAAATGTAGTGCTTTCCAGGATAGTTCCTTCC; ATACTCCTTCTGTTCCTTTCATTGTGCCCCTCTCTGTGATTATTTGTCCTGATCGTGTTG; GAGTTTTCCTAGACTATGAGGCTTGCACTGTCTCATTCTTCAATATCACAAACCATGGAT; TTCTCATCTATAAGTTTTCTCACTGTTCTTTTTCTCAGCCTGTATTTCCATATTTAAATC; CTAGAAAATGTGGAGTCCCCATGACTCTGTGCTCACCAAGCTCTTGAACCTTCTTACACA; CTCAGCCCCTTCTGTACAGCACCTCTTGTCCAGGTGCATCTCATACACCTGAACTCATTT; GCATCATTTTAACCATCTTTTCCTTGCTGTCTCCCTTCTTTCTATTTGAACGTCCTTCAC; TCATCAGTAAAATGTAATAATTGCCTTGTGCCATATTGTCCCCAATATTTTATTGACATT; TGATAGCAATTTTTTTCATCATTTTCCGTACTCCTAAGGAAAACTGACCTATACCTCATA; AAATGAGACCGCTATTTAGGTATTACTTCTGCCAGATATTTATCACCCAATTGCCTCTGA; CACTGACTAAGAAGATGAAGAAAAGCTTTTCAACAGCCTTTCTATATCATCGTGTGATAA; TTGTTCACCAATGAATGAGTCCTTAGCCCTGTGTCAGTTTACCCTCGATGCCCTTATTTG; TGAGTTAAAGAGAAAATATCATAAATGGTATACTCTTAAGTATAGAGGTTTTGTATCTAG; AGGATCTCAGTTCAACTCCTGTCTCTCCATATACCAGCAGTGTAACTGTGAATAACATAC; TTAAATGGCTGTGCTTATTTCCTTTTCTTTTCTTTTTTCTTTTTTTTTTTTTTTGAGATG; AAGTTTTGCTCTTGTTCCCCAGGCTGGAGTGCAATGGCACGATCTCGGTTCACTGCAACC; TCCACCTCTCAGATTCAAGCAATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAG; GTGCCCACCACCACCCCTGGCTAAATTTGTATTTTCAGTAGAGACGGGGTTTCCCCATGT; TGGTTAGGCTCGTCTAGAACCTCTGACCTCAGGTGATCCACCCGCCTCGGCCTCCCAAAG; TGCTGGGATTACAGGCGTGAGCCACGGCGCCCAGCCTGTGCTTATTTTCTTAAAATAATT; TTTGTATTAAAAACTTCACATTAAATAAGTGCTAATGTTTTATTGCATAGTAGGGTGACT; AGAGTTAACAATAACCTATTGCATATATTTTGAAATAGCTAGAAGAGAGGATTTTGAAAG; TTCTCAACACAAAGAAATGACACATATTTGAGGTGATGGATATGCTAATTACCCTGGTTC; GGTTATTACGCAATGTATACATGTATCAAAACATCACACTGTACCACATAAATATGTATA; TTTATTATTTGTCAATTAAAAGCAAAATAAAACAAAAAACCTTCATCTAATACTTTGGAT; CATTGTGAAAAAATAAATTCCTGAAGTATAAAGCATCTXXXXXXXXXXXXXXXXAACTCG; AC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033034 unedited; CAAATTTATGTATACGACTCACTATAGGGCGGCCGCGNAATTCGCACCAGCCAGGACAGG; CAGGAGCAGTGGAATAGCTAGTATGGCTTCTGGAATCCTGGTTAATGTAAAGGAGGAGGT; GACCTGCCCCATCTGCCTGGAACTCCTGACACAACCCCTGAGCCTGGACTGCGGCCACAG; CTTCTGCCAAGCATGCCTCACTGCAAACTACAAGAAGTCCATGCTAGACAAAGGAGAGAG; TAGCTGCCCTGTGTGCCGGATCAGTTACCAGCCTGAGAACATACGGCCTAATCGGCATGT; AGCCAACATAGTGGAGAAGCTCAGGGAGGTCAAGTTGAGCCCAGAGGGGCAGAAAGTTGA; TCATTGTGCACGCCATGGAGAGAAACTTCTACTCTTCTGTCAGGAGGACGGGAAGGTCAT; TTGCTGGCTTTGTGAGCGGTCTCAGGAGCACCGTGGTCACCACACGTTCCTCACAGAGGA; GGTTGCCCGGGAGTACCAAGTGAAGCTCCAGGCAGCTCTGGAGATGCTGAGGCAGAAGCA; GCAGGAAGCTGAAGAGTTAGAAGCTGACATCAGAGAAGAGAAAGCTTCCTGGAAGACTCA; AATACAGTATGACAAAACCAACGTCTTGGCAGATTNTGAGCAACTGAGAGACATCCTGGA; CTGNGAGGAGAGCAATGAGCTGCANAACCTGNAGAAAGAGGANGAAGACATTCTGAAAAG; CCTTACGAACTCTGAAACTGAGATGGTGCAGCAGACCCAGTCCCTGAGAGAGCTCATCTC; AGATCTGGAGCATCGGCTGCAGGGGTCAGTGATGGAGCTGCCTCAAGGTGTTGGATTGCG; TCATAAAAAGAGCTGACAGATGTCCGACGCTACTNGGGTTGATGTGACAGTGGCTCCAAA; CAACATTAATGGGCTGCCATTCCTGAGAATAGAGAAAGGGAGCCTTCGGAACCCAGTAAA; TATATGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033034 unedited; TTGGACCGCGGCCGCAATCTACAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTAAAAGGCTTAAAACTTCGAGGAATTTATTTTTTACCAAGGATCCC; AAAGTTTTAAAGGAGGGGTTTTGGTTTAATTTGGCTTTAAATGGCCAAAAAAAAAATATC; CCTATTTATGGGGCCCAGGGGGAGGTTTTGAAACATGTTTACTTTGGGAAAAACCCGACC; CAGGGGAATAACCAAACCCTTTTTTTTAAAAAGGGGCCATTTTTTTGGGGGGAAAACTTT; AAAAATCCTTTTTTTTAGCTATTCCAAAATATATGCAAAAGGTTATTGTTAACCTTAGCC; CCCCTCCTTTGCAAAAAAACATTCCCCCTTATTTAAGGGGAAGCTTTTAAATCAAAAATT; ATTTTAAAAAAAAAACCCCCGGCTGGGCCCCGGGGCTCCCCCCTGAAACCCCACACTTGG; GGAGGCCCAGGCGGGGGGACCCCCTGGGGTCACAGGCTCAACACCACCCCCACACACATG; GGGCACACCCCCCCTTCCAATGAACCACCAACCCCAACCACCGGGTGGGGGGGGGCCCCT; CCACACCCCCCCCTGGCGCGGCCCGCCCCGAACACCCTTCTCTCCCTCCACCCGAGCGGC; CACACCACCCCACAACCTCCCCCCTCCCCCCACACCGGCCCCATCCACCCACCATCTTTC; TCTCTCCCCCTCAATCCCAACCCCTCCTTTACGCCCACCCCCCTCTCCTCCCCCCCTTCC; TCCCCCCCCACCCCTTCCCCTATCTCACCACCCCCCGCTAACAACTCCCTCACTCACACT; CCCCCCCCCCCCCCCCAAACCACCCTCCCCCCCCCCCCCCCCCCAAAACCCAATATCCTC; CCCCCTCCCCCCCCATACCCATTCCCCCACCCCCCCCACCTTACTCTACCCCACTTCCAC; CCCCCACTCCCTCCCCCCACCCCCCCACCCCCTCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_033034
• Insert Size: 2750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_033034.1, NP_149023.1
• RefSeq Size: 2990 bp
• RefSeq ORF: 1482 bp
• Locus ID: 85363
• UniProt ID: Q9C035
• Cytogenetics: 11p15.4
• Domains: RING, SPRY, zf-B_box
• Protein Families: Druggable Genome
• Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009]; Transcript Variant: This variant (alpha) encodes the longest isoform (alpha). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.