PHF17 (JADE1) (NM_024900) Human Untagged Clone

SKU SC111825

PHF17 (untagged)-Human PHD finger protein 17 (PHF17), transcript variant S

Product Data

• Type: Human Untagged Clone
• Target Symbol: PHF17
• Synonyms: PHF17
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024900, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAAACGAGGTCGCCTTCCCAGCAGCAGTGAGGATTCTGACGACAATGGCAGCCTGTCAACTACTTGGT; CCCAGAATTCCCGATCCCAGCATAGGAGAAGCTCCTGCTCCAGACATGAAGATCGAAAGCCTTCAGAGGT; GTTTAGGACAGACCTGATCACTGCCATGAAGTTGCATGACTCCTACCAGCTGAATCCGGATGAGTACTAT; GTGTTGGCAGATCCCTGGAGACAGGAATGGGAGAAAGGGGTCCAGGTGCCTGTGAGCCCGGGGACCATCC; CTCAGCCTGTGGCCAGGGTTGTGTCTGAAGAGAAATCCCTCATGTTCATCAGGCCCAAGAAGTACATCGT; GTCATCAGGCTCTGAGCCTCCCGAGTTGGGCTATGTGGACATCCGGACGCTGGCTGACAGCGTGTGTCGC; TATGACCTCAATGACATGGATGCTGCATGGCTGGAACTGACCAATGAAGAATTTAAGGAGATGGGAATGC; CTGAACTAGATGAATACACCATGGAGAGGGTCCTAGAGGAATTTGAGCAGCGATGCTACGACAATATGAA; TCATGCCATAGAGACTGAGGAAGGCCTGGGGATCGAATATGATGAAGATGTTGTCTGTGATGTCTGCCAG; TCTCCTGATGGTGAGGACGGCAATGAGATGGTGTTCTGTGACAAATGCAACATCTGTGTGCACCAGGCCT; GTTATGGAATCCTCAAGGTACCAGAGGGCAGCTGGCTGTGCCGGACATGTGCCCTGGGGGTTCAGCCAAA; ATGTCTGCTGTGTCCGAAGAAGGGTGGAGCTATGAAGCCCACCCGTAGCGGAACCAAGTGGGTCCACGTT; AGCTGTGCTCTGTGGATCCCTGAGGTGAGCATTGGCAGCCCAGAGAAGATGGAGCCCATCACCAAGGTGT; CACACATTCCCAGCAGCCGGTGGGCGCTAGTGTGCAGCCTCTGCAATGAGAAGTTTGGGGCCTCTATACA; GTGCTCTGTGAAGAACTGCCGCACAGCCTTCCATGTGACCTGTGCTTTTGACCGGGGCCTGGAGATGAAG; ACCATCTTAGCAGAGAATGATGAAGTCAAGTTCAAGTCCTATTGCCCAAAGCACAGCTCACATAGGAAAC; CCGAGGAGAGTCTTGGCAAGGGGGCTGCACAGGAGAATGGGGCCCCTGAGTGTTCCCCCCGGAATCCGCT; GGAGCCCTTTGCCAGCCTTGAGCAGAACCGGGAGGAGGCCCACCGGGTGAGTGTCCGTAAGCAGAAGCTG; CAGCAGTTGGAGGATGAGTTCTACACCTTCGTCAACCTGCTGGATGTTGCCAGGGCTCTGCGGCTGCCTG; AGGAAGTAGTGGATTTCCTGTACCAGTACTGGAAGTTGAAGAGGAAGGTCAACTTCAACAAGCCCCTGAT; CACCCCAAAGAAAGATGAAGAGGACAATCTAGCCAAGCGGGAGCAGGATGTCTTATTTAGGAGGCTGCAG; CTGTTCACGCACCTGCGGCAGGACCTGGAGAGGGTAATGATTGACACTGACACCTTATAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024900 unedited; CGGCAGATTTGTCTACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCAGAACCGGG; AGGAGGCCCACCGGGTGAGTGTCCGTAAGCAGAAGCTGCAGCAGTTGGAGGATGAGTTCT; ACACCTTCGTCAACCTGCTGGATGTTGCCAGGGCTCTGCGGCTGCCTGAGGAAGTAGTGG; ATTTCCTGTACCAGTACTGGAAGTTGAAGAGGAAGGTCAACTTCAACAAGCCCCTGATCA; CCCCAAAGAAAGATGAAGAGGACAATCTAGCCAAGCGGGAGCAGGATGTCTTATTTAGGA; GGCTGCAGCTGTTCACGCACCTGCGGCAGGACCTGGAGAGGGTTCGGAACCTCACTTACA; TGGTGACCCGCAGGGAAAAGATTAAACGGTCTGTGTGCAAAGTCCAGGAACAGATATTCA; ATCTTTACACTAAGCTNTTGGAGCTAGACAGAGTTTCAGGTGGNGCCTTCTTCCTGCTCC; CTCCTCTCACTGGCAAACATGCTTTTTGTTACCAGTCTTCTGTTGGCCTGATGCTCCCCA; GAAGAGGACCTGAATTGGCATTTTGCATCTTTAGAAAACCAAAGGGCCCTCCCTTTGTTC; ATTCCTTAAAAAGCCCCTAAGCAAGACCTTTTGCAAAAAACCCTCTGAAGGAAAGGCAAA; CCCTTTTTAAACCACCCCAACCTTGTTGGAAAAGGGGGGGGGAGGGGGTCCCCACAAAAC; TTTTTTGGGTTTAAAAGAACATTTTTTGAAACCCTCTTTTTTATAACCCCCACAAAAAAA; GGGGGTGTGTGTGCCCCCCCCTCGGGAAAAAAAGAAACACCCTTTTTTTTGGGGGCCCCT; CTAGGGGGTGTTATGGGCGGCTTTTTTTATAGAAGGACCCCCCCTTCTCTCCCCACAAAT; TGTCTCCCGGAGATGTGGCCACACAAAACCCCCCCCTCTCTGTGGGGGGGCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_024900
• Insert Size: 2350 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_024900.2, NP_079176.2
• RefSeq Size: 4204 bp
• RefSeq ORF: 1530 bp
• Locus ID: 79960
• UniProt ID: Q6IE81
• Cytogenetics: 4q28.2
• Domains: PHD
• Protein Families: Druggable Genome, Transcription Factors
• Summary: Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator, it may also promote acetylation of nucleosomal histone H4 by KAT5. Promotes apoptosis. May act as a renal tumor suppressor. Negatively regulates canonical Wnt signaling; at least in part, cooperates with NPHP4 in this function.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) lacks two exons and its 3'-terminal exon extends past a splice site that is used in variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Variants 2 and 6 encode the same isoform.