POFUT1 (NM_015352) Human Untagged Clone

SKU SC111782

POFUT1 (untagged)-Human protein O-fucosyltransferase 1 (POFUT1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: POFUT1
• Synonyms: DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT; OFUCT1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111782 sequence for NM_015352 edited (data generated by NextGen Sequencing); ATGGGCGCCGCCGCGTGGGCACGGCCGCTGAGCGTGTCTTTCCTGCTGCTGCTTCTGCCG; CTCCCGGGGATGCCTGCGGGCTCCTGGGACCCGGCCGGTTACCTGCTCTACTGCCCCTGC; ATGGGGCGCTTTGGGAACCAGGCCGATCACTTCTTGGGCTCTCTGGCATTTGCAAAGCTG; CTAAACCGTACCTTGGCTGTCCCTCCTTGGATTGAGTACCAGCATCACAAGCCTCCTTTC; ACCAACCTCCATGTGTCCTACCAGAAGTACTTCAAGCTGGAGCCCCTCCAGGCTTACCAT; CGGGTCATCAGCTTGGAGGATTTCATGGAGAAGCTGGCACCCACCCACTGGCCCCCTGAG; AAGCGGGTGGCATACTGCTTTGAGGTGGCAGCCCAGCGAAGCCCAGATAAGAAGACGTGC; CCCATGAAGGAAGGAAACCCCTTTGGCCCATTCTGGGATCAGTTTCATGTGAGTTTCAAC; AAGTCGGAGCTTTTTACAGGCATTTCCTTCAGTGCTTCCTACAGAGAACAATGGAGCCAG; AGATTTTCTCCAAAGGAACATCCGGTGCTTGCCCTGCCAGGAGCCCCAGCCCAGTTCCCC; GTCCTAGAGGAACACAGGCCACTACAGAAGTACATGGTATGGTCAGACGAAATGGTGAAG; ACGGGAGAGGCCCAGATTCATGCCCACCTTGTCCGGCCCTATGTGGGCATTCATCTGCGC; ATTGGCTCTGACTGGAAGAACGCCTGTGCCATGCTGAAGGACGGGACTGCAGGCTCGCAC; TTCATGGCCTCTCCGCAGTGTGTGGGCTACAGCCGCAGCACAGCGGCCCCCCTCACGATG; ACTATGTGCCTGCCTGACCTGAAGGAGATCCAGAGGGCTGTGAAGCTCTGGGTGAGGTCG; CTGGATGCCCAGTCGGTCTACGTTGCTACTGATTCCGAGAGTTATGTGCCTGAGCTCCAA; CAGCTCTTCAAAGGGAAGGTGAAGGTGGTGAGCCTGAAGCCTGAGGTGGCCCAGGTCGAC; CTGTACATCCTCGGCCAAGCCGACCACTTTATTGGCAACTGTGTCTCCTCCTTCACTGCC; TTTGTGAAGCGGGAGCGGGACCTCCAGGGGAGGCCGTCTTCTTTCTTCGGCATGGACAGG; CCCCCTAAGCTGCGGGACGAGTTCTGA; ; Clone variation with respect to NM_015352.1;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015352 unedited; AACTCCCGCCCCGTTCCCGCATTACTGCGGTAGGCGTGCACGGTGGGAGGTCTATATAAG; CAGAGTTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC; GAATTCGGCACGAGGCCCGACTGTGCGCCGCGGCTGGCTCGGGTTCCCGGGCCGACATGG; GCGCCGCCGCGTGGGCACGGCCGCTGAGCGTGTCTTTCCTGCTGCTGCTTCTGCCGCTCC; CGGGGATGCCCGCGGGCTCCTGGGACCCGGCCGGTTACCTGCTCTACTGCCCCTGCATGG; GGCGCTTTGGGAACCAGGCCGATCACTTCTTGGGCTCTCTGGCATTTGCAAAGCTGCTAA; ACCGTACCTTGGCTGTCCCTCCTTGGATTGAGTACCAGCATCACAAGCCTCCTTTCACCA; ACCTCCATGTGTCCTACCAGAAGTACTTCAAGCTGGAGCCCCTCCAGGCTTACCATCGGG; TCATCAGCTTGGAGGATTTCATGGAGAAGCTGGCACCCACCCACTGGCCCCCCTGAGAGC; CGGGTGGCATACTGCTTTGAGGTGGCAGCCCAGCGAAGCCCAGATAAGAAGACGTGCCCC; ATGAAGGAAGGAAACCCCTTTGGCCCATTCTGGGATCAGTTCCATGTGAGTTCAACAAGT; CGGAGCTTTTACAGGCTTTCCCTCAGTGCTTCTACCGAGAACATGGAGCCCGAGATTTCC; TCCAAAGAACATTCGGTGCTTGGCCTTGCAGGAACCCCAGCCCAGTTCCCCGCCCTACAG; GAACACAGGCCCCTCAGCAACACATGGTATGGTCACCACAAATGGTGAAGACCGGAGAAG; GCCCACATCATTCCCCACCTTGTCCGGGCCTATGGGGGCCCCATATTTGGCCCCGGCCTC; TGACTTGAAAAACACCGGGCCCTGCTCTAAGAACGGGAATGAAGCGCCCCACTTATTGCC; CTCCTCCAAGGGTGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015352 unedited; TTTATTTTCTCTTTTNNTTTTTTNNTTTTTTNNTTTTTTTTTTTNTTTTTTTNTNNTTTT; TTTNNTTTTTTTTTTTTNTTTTTTTTTNTTTTTAAAAAAAAAAACCTTTTTCCCGGCTCC; CTCCCCCCGGGGGAAAACCTGGTCCCCTTTTAAGTAAAACCCCGGGGCCTTAAATTGGTT; TTTTTACCTGGTTAAAACCTTGTAAATTTTTGGATCTTTGAAAAAAACTGGAAAATATTT; TCCTTTGTTCAAAATCTAATTTTTTCCCCCCCCCTTTAATGCAATGGCCTTTGTCTTTTG; GGGAAGGGGGGCCCTGGGGTCCTATTCTAGGGGGGCCCAACCCCCCATTTTTTTGCCTGG; AACCCTGTTAAAAACCCCGGCCGGGGCTTTTTGGTTTTACCCTTTTTTTTTCCAACCCCC; TTAACACTGGCCCATTTAAGGGGGCCCTCAAAATGGGAATTAACTTATGCCCCTTTTTTG; GTTAAAACCCTAGGGGGGTTCCTGGTTTTCTCCCACTAAAAACTTCCTGCCCTGGCCCGC; CCGGCTGGAAAATGGCACCTTCCCCGGTCTGACCCGGTGGTTGGGTCCCCGGCTATGGCT; GGCTTTCCAAACATGCTGGGCCCCTGGGGTGTTTTTTAGAACACCCTCCAATTTTGGGGC; CTTTTGACCGGCTGGTTTCTCTGGCCGGAAAGGGCCCCCTCCCCCCCTTTTGAAAGCTCT; TTAGAAAGGGGCTTCCCCGGGCCCCCCTAAAAAAAATCCCCCCCCCCTTTTTGGGCCCAA; CCCCTTTTTTTTTTTATAGCCCTTTTTAAATGTGGCCCACGGACATTTTTACACCCCCCC; CCTTTCAAAAGGCAATCCCTGGGAAAAAAACTTTTCCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_015352
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015352.1, NP_056167.1
• RefSeq Size: 5249 bp
• RefSeq ORF: 1167 bp
• Locus ID: 23509
• UniProt ID: Q9H488
• Cytogenetics: 20q11.21
• Protein Families: Druggable Genome, Transcription Factors
• Summary: This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the longer isoform (1) of this protein.