LIAS (NM_006859) Human Untagged Clone

SKU SC111760

LIAS (untagged)-Human lipoic acid synthetase (LIAS), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: LIAS
• Synonyms: HGCLAS; HUSSY-01; LAS; LIP1; LS; PDHLD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006859, the custom clone sequence may differ by one or more nucleotides; ; ; ATGTCTCTACGCTGCGGGGATGCAGCCCGCACCCTGGGGCCCCGGGTATTTGGGAGATATTTTTGCAGCC; CAGTCAGACCGTTAAGCTCCTTGCCAGATAAAAAAAAGGAACTCCTACAGAATGGACCAGACCTTCAAGA; TTTTGTATCTGGTGATCTTGCAGACAGGAGCACCTGGGATGAATATAAAGGAAACCTAAAACGCCAGAAA; GGAGAAAGGTTAAGACTACCTCCATGGCTAAAGACAGAGATTCCCATGGGGAAAAATTACAATAAACTGA; AAAATACTTTGCGGAATTTAAATCTCCATACAGTATGTGAGGAAGCTCGATGTCCCAATATTGGAGAGTG; TTGGGGAGGTGGAGAATATGCCACCGCCACAGCCACGATCATGTTGATGGGTGACACATGTACAAGAGGT; TGCAGATTTTGTTCTGTTAAGACTGCAAGAAATCCTCCTCCACTGGATGCCAGTGAGCCCTACAATACTG; CAAAGGCAATTGCAGAATGGGGTCTGGATTATGTTGTCCTGACATCTGTGGATCGAGATGATATGCCTGA; TGGGGGAGCTGAACACATTGCAAAGACCGTATCATATTTAAAGGAAAGGAATCCAAAAATCCTTGTGGAG; TGTCTTACTCCTGATTTTCGAGGTGATCTCAAAGCAATAGAAAAAGTTGCTCTGTCAGGATTAGATGTGT; ATGCACATAATGTAGAAACAGTCCCGGAATTACAGAGTAAGGTTCGTGATCCTCGGGCCAATTTTGATCA; GTCCCTACGTGTACTGAAACATGCCAAGAAGGTTCAGCCTGATGTTATTTCTAAAACATCTATAATGTTG; GGTTTAGGCGAGAATGATGAGCAAGTATATGCAACAATGAAAGCACTTCGTGAGGCAGATGTAGACTGCT; TGACTTTAGGACAATATATGCAGCCAACAAGGCGTCACCTTAAGGTTGAAGAATATATTACTCCTGAAAA; ATTCAAATACTGGGAAAAAGTAGGAAATGAACTTGGATTTCATTATACTGCAAGTGGCCCTTTGGTGCGT; TCTTCATATAAAGCAGGTGAATTTTTCCTGAAAAATCTAGTGGCTAAAAGAAAAACAAAAGACCTCTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006859 unedited; TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGTTAGCGATCCCTCAACCC; CTGCACTGCGCTAGTCCTAAAGAGGAAATGTCTCTACGCTGCGGGGATGCAGCCCGCACC; CTGGGGCCCCGGGTATTTGGGAGATATTTTTGCAGCCCAGTCAGACCGTTAAGCTCCTTG; CCAGATAAAAAAAAGGAACTCCTACAGAATGGACCAGACCTTCAAGATTTTGTATCTGGT; GATCTTGCAGACAGGAGCACCTGGGATGAATATAAAGGAAACCTAAAACGCCAGAAAGGA; GAAAGGTTAAGACTACCTCCATGGCTAAAGACAGAGATTCCCATGGGGAAAAATTACAAT; AAACTGAAAAATACTTTGCGGAATTTAAATCTCCATACAGTATGTGAGGAAGCTCGATGT; CCCAATATTGGAGAGTGTTGGGGAGGTGGAGAATATGCCACCGCCACAGCCACGATCATG; TTGATGGGTGACACATGTACAAGAGGTTGCAGATTTTGTTCTGTTAAGACTGCAAGAAAT; CCTCCTCCACTGGATGCCAGTGAGCCCTACAATACTGCAAAGGCAATTGCAGAGTGGGGT; CTGGATTATGTTGTCCTGACATCTGTGGATCGAGATGATATGCCTGATGGGGGAGCTGAA; CACATTGCANAGACCGTATCATATTTAAAGGAAAGGAATCCAAAAATCCTTGTGGAGTGT; CTTACTNCTGATTNTCGAGGTGATCTCAAAGCAATAGAAAAAGTTGCTCTGTCAGGATTA; GATGTGTATGCACATAATGTAGAAACAGTCCCGGAATTTACAGAGTGATTACGTGTACAA; AGTATGGTTGGAAGTTAGGCGGTCAAATATGCATATATTCTGCCTTTCCAGGCTGAACTT; GCCATGACTTTACAAGTAACNTATTCTTTTTGTTTTAGCAACTCATCTGTCTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006859 unedited; AAGCTGTGNACCGCGGCCCGCTTTCTANGATCGAGTTTTTCTTTTTTTTTTTTATTTATT; TGAGATGGAGTTTTGCTCTTGTCACCCAGGCTGGAGGGCAATGGAGCGATCCTGGCTCAC; CGCAACCTCCCCCTCCCAGGCTCAAGTGATTCTCCTGCCTCAGCCCCCCGAGTAGCTGGG; ACCACAGGCACCCACCACCACGCCTGACTAATTTTTGTGTTTTTAGTGGAGACAGGATTT; CACCATGTTGGCCAGGCTGGTCTCGAACTCCCGACCTCAGGTGATCCACCCGCCTCGGCC; TCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTATGCCTGGCTATTGACATTTTTTTTT; TTAAGCAAAGAGGTGGGGCACATCCACTGCAGTCCAGGCATTCTGGCACCACCTCTGTTA; TTAACTGGAATCAAATTTTAAAAATTTCTGTGATCTTGAAGGTCTTGTTGAAGTTTTAGA; GGTCTTTTGTTTTTCTTTTAGCCACTAGATTTTTCAGGAAAAATTCACCTGCTTTATATG; AAGAACGCACCAAAGGGCCACTTGCAGTATAATGAAATCCAAGTTCATTTCCTACTTTTT; CCCAGTATTTGAATTTTTCAGGAGTAATATATTCTTCAACCTTAAAGTGACGCCTTGTTG; GCTGCATATATTGTCCTAAAGTCAAGCAGTCTACATCTGCCTCACGAAGTGCTTTCATTG; TTGCATATACTTGCTCATCATTCTCGCCTTAACCCAACATTATAGATGTTTTAAGAAATA; CATCAGGCTGAACCTTCTTGGCATGTTTCAGTACACGTAGGGACTGATCAAAATGGCCCG; AGGATCACGAACCTAATCACCGCAACCTCCGGCTNCCGGACTCAAGTGATCTTTTCCTGC; TTAGCCTACCGACCGCTGGAACTAAGGCTGGTGCCCCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_006859
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006859.2, NP_006850.2
• RefSeq Size: 1743 bp
• RefSeq ORF: 1119 bp
• Locus ID: 11019
• UniProt ID: O43766
• Cytogenetics: 4p14
• Protein Pathways: Lipoic acid metabolism, Metabolic pathways
• Summary: The protein encoded by this gene belongs to the biotin and lipoic acid synthetases family. Localized in the mitochondrion, this iron-sulfur enzyme catalyzes the final step in the de novo pathway for the biosynthesis of lipoic acid, a potent antioxidant. The deficient expression of this enzyme has been linked to conditions such as diabetes, atherosclerosis and neonatal-onset epilepsy. Alternative splicing occurs at this locus, and several transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Aug 2020]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).