TANK (NM_004180) Human Untagged Clone

SKU SC111743

TANK (untagged)-Human TRAF family member-associated NFKB activator (TANK), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: TANK
• Synonyms: I-TRAF; ITRAF; TRAF2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111743 sequence for NM_004180 edited (data generated by NextGen Sequencing); ATGGATAAAAACATTGGCGAGCAACTCAATAAAGCGTATGAAGCCTTCCGGCAGGCATGC; ATGGATAGAGATTCTGCAGTAAAAGAATTACAGCAAAAGACTGAGAACTATGAGCAGAGA; ATACGTGAACAACAGGAACAGCTGTCACTTCAACAGACTATTATTGACAAGCTAAAATCT; CAGTTACTTCTTGTGAATTCCACTCAAGATAACAATTATGGCTGTGTTCCTCTGCTTGAA; GACAGTGAAACAAGAAAGAATAATTTGACTCTTGATCAGCCACAAGATAAAGTGATTTCA; GGAATAGCAAGAGAAAAACTACCAAAGGTAAGAAGACAAGAGGTTTCTTCTCCTAGAAAA; GAAACTTCAGCAAGGAGTCTTGGCAGTCCTTTGCTCCATGAAAGGGGTAATATAGAGAAG; ACTTTCTGGGATCTGAAAGAAGAATTTCATAAAATATGCATGCTAGCAAAAGCACAGAAA; GACCACTTAAGCAAACTTAATATACCAGACACTGCAACTGAAACACAGTGCTCTGTGCCT; ATACAGTGTACGGATAAAACAGATAAACAAGAAGCGCTGTTTAAGCCTCAGGCTAAAGAT; GATATAAATAGAGGTGCACCATCCATCACATCTGTCACACCAAGAGGACTGTGCAGAGAT; GAGGAAGACACCTCTTTTGAATCACTTTCTAAATTCAATGTCAAGTTTCCACCTATGGAC; AATGACTCAACTTTCTTACATAGCACTCCAGAGAGACCCGGCATCCTTAGTCCTGCCACG; TCTGAGGCAGTGTGCCAAGAGAAATTTAATATGGAGTTCAGAGACAACCCAGGGAACTTT; GTTAAAACAGAAGAAACTTTATTTGAAATTCAGGGAATTGACCCCATAGCTTCAGCTATA; CAAAACCTTAAAACAACTGACAAAACAAAGCCCTCAAATCTCGTAAACACTTGTATCAGG; ACAACTCTGGATAGAGCTGCGTGTTTGCCACCTGGAGACCATAATGCATTATATGTAAAT; AGCTTCCCACTTCTGGACCCATCTGATGCACCTTTTCCCTCACTCGATTCCCCGGGAAAA; GCAATCCGAGGACCACAGCAGCCCATTTGGAAGCCCTTTCCTAATCAAGACAGTGACTCG; GTGGTACTAAGTGGCACAGACTCAGAACTGCATATACCTCGAGTATGTGAATTCTGTCAA; GCAGTTTTCCCACCATCCATTACATCCAGGGGGGATTTCCTTCGGCATCTTAATTCACAC; TTCAATGGAGAGACTTAA; ; Clone variation with respect to NM_004180.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004180 unedited; CACCATTTGTATCCGACTCATATAGGCGGCCGCGCAATTCGCACGAGGGTACCTGTCATT; TACTCCATCCTTTATAGTGATGCTACAGGACGAAGAGGAATGGATAAAAACATTGGCGAG; CAACTCAATAAAGCGTATGAAGCCTTCCGGCAGGCATGCATGGATAGAGATTCTGCAGTA; AAAGAATTACAGCAAAAGACTGAGAACTATGAGCAGAGAATACGTGAACAACAGGAACAG; CTGTCACTTCAACAGACTATTATTGACAAGCTAAAATCTCAGTTACTTCTTGTGAATTCC; ACTCAAGATAACAATTATGGCTGTGTTCCTCTGCTTGAAGACAGTGAAACAAGAAAGAAT; AATTTGACTCTTGATCAGCCACAAGATAAAGTGATTTCAGGAATAGCAAGAGAAAAACTA; CCAAAGGTAAGAAGACAAGAGGTTTCTTCTCCTAGAAAAGAAACTTCAGCAAGGAGTCTT; GGCAGTCCTTTGCTCCATGAAAGGGGTAATATAGAGAAGACTTTCTGGGATCTGAAAGAA; GAATTTCATAAAATATGCATGCTAGCAAAAGCACAGAAAGACCACTTAAGCAAACTTAAT; ATACCAGACACTGNCACTGAAACACAGTGCTCTGTGCCCTATACAGTGTACGGATTAAAC; AGATAAACAAGAAGCGCTGTTTAAGCCTCANGGCTAAGATGATATAAATAGAAGTGCACC; ATCCATCACATCTGTCACACCCAGAGGACTGTGCAGAGATGAAGAAACACCTCTTTTGAA; TCACTTTCTAAATACATGTCAAGTTTCCACTATGGACAATGACTCAACTTCTTACATAGC; ACTCCAGAGAGACCCGGCATCCTTATTCCTGCACGTCTGGAGCAGGGTGCCAGGAAAATT; TATTATGGAGTTCAAAAACAACCCAGGGAACTTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004180 unedited; CGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTACCTCTTAATATTTAATTAAT; TTCATCATGTAGCTATACACATAGTAAAAATTAAAGCCATGTATATTATTTAAAAATCTA; AAGTGTTTAAATAATTCTACCAGTTGTTTTATCACTGGACAAGAAATGTATTAGGTTTGA; TATTTACAAAGATGTTTATGTTGTCTGCTATTTTCTATTATAGGTTTATTCAAAGAGAAT; ATCACCTATAGCATAAATAATTAAATTTTTCAATAAAAACAAACTAAAAAAACCCTAGAA; ACATTTGACATCACCAAATTCAACAGCTCTCACTAGAAATCCAAGCAATAAACTTAGATA; TTTGAAATAAACATAAATTATGATTATATAACTCTAAGTCACATACATAATTTTGAATTA; TAGTAATAGCACTGTAAACATGAATACAAAGGATTACAGTTTTATACAGAATTTTTTTTG; TTTTTCTTCTTTTAGGCTTATTTCTCCTTGAAAGAACAGAATGATCTTTTAAAAAATAAG; AGTTATATAGTAAATCCAGAAAAAAATTCAAATAGCTGTGAAACTAGATTTTCCTATAAA; TGATCTTGAATTTAGTTTACAATCAAGTATTTATAGTATTAAAAACCCAAAATCATCACA; TAGAACTTGATATGTCTGTTTTCAAATGTGTCTTAAGTCTCTTCATTGAAGTGTGAAATA; AGATGCCCGAAGAAATCCCNCCTGGNATGTATGGATGGTGGGAAAACTGCTTGACAGAAT; CACATACTCGAGTATATGCAGTTCTGAGTCTGTGCCACTTANTACACCGAGTTACTGTCT; TGATTANGAAGGGGCTTNCAATGGNCTGCTGTGGTCCTCGNATGCTTTTCCCGGGNATCG; AGTGAGGGAAAAGTGCATCNATGGTCCAGAANTGGAAGCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_004180
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004180.2, NP_004171.2
• RefSeq Size: 2089 bp
• RefSeq ORF: 1278 bp
• Locus ID: 10010
• UniProt ID: Q92844
• Cytogenetics: 2q24.2
• Protein Families: Druggable Genome
• Protein Pathways: RIG-I-like receptor signaling pathway
• Summary: The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor superfamily. The protein encoded by this gene is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby inhibiting TRAF function by sequestering the TRAFs in a latent state in the cytoplasm. For example, the protein encoded by this gene can block TRAF2 binding to LMP1, the Epstein-Barr virus transforming protein, and inhibit LMP1-mediated NF-kappa-B activation. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]; Transcript Variant: This variant (1) is the longer transcript and encodes the longer isoform (a).