RHOBTB1 (NM_014836) Human Untagged Clone

SKU SC111741

RHOBTB1 (untagged)-Human Rho-related BTB domain containing 1 (RHOBTB1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: RHOBTB1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111741 sequence for NM_014836 edited (data generated by NextGen Sequencing); ATGGACGCTGACATGGACTACGAAAGACCCAACGTTGAAACTATCAAATGTGTGGTCGTG; GGTGACAATGCCGTGGGGAAGACGCGCTTGATCTGTGCCAGGGCGTGCAACACCACACTC; ACGCAGTATCAGCTGCTGGCCACCCACGTGCCAACAGTGTGGGCGATTGACCAGTACCGC; GTGTGCCAGGAGGTCTTGGAGCGTTCTCGGGATGTTGTTGATGAAGTGAGTGTTTCTCTC; AGGCTTTGGGATACTTTTGGTGATCATCACAAAGACAGACGCTTTGCATATGGCAGGTCT; GATGTTGTGGTCCTCTGTTTTTCGATTGCTAATCCCAATTCCCTAAATCATGTGAAAAGC; ATGTGGTATCCAGAAATCAAGCACTTTTGCCCTCGAACACCCGTTATCCTTGTTGGGTGC; CAGCTTGATCTCCGCTATGCCGACCTGGAAGCTGTTAATCGAGCCAGGCGCCCGTTAGCA; AGGCCCATAAAGAGAGGGGATATTTTGCCCCCAGAAAAAGGCCGAGAGGTAGCAAAGGAA; CTTGGCTTACCATACTATGAAACAAGCGTGTTTGACCAGTTTGGTATCAAGGATGTGTTT; GACAATGCAATCCGAGCAGCGCTGATTTCCCGCAGGCACCTGCAATTCTGGAAATCCCAC; CTAAAGAAAGTCCAGAAACCTTTACTTCAGGCACCCTTCCTACCTCCAAAAGCCCCTCCA; CCGGTCATCAAAATTCCAGAGTGTCCTTCCATGGGGACAAATGAAGCTGCCTGTTTACTG; GACAATCCTCTATGTGCCGATGTTCTGTTCATCCTTCAGGACCAGGAACACATCTTTGCA; CATCGAATTTACCTCGCTACCTCTTCTTCCAAATTTTATGATCTGTTTTTAATGGAATGT; GAAGAATCCCCAAATGGGAGTGAAGGAGCCTGTGAGAAAGAGAAGCAGAGCAGAGATTTC; CAGGGGCGGATATTGAGTGTCGACCCAGAGGAAGAAAGGGAGGAGGGCCCGCCTAGGATT; CCTCAGGCCGACCAGTGGAAGTCTTCAAACAAGAGCCTGGTGGAGGCTCTGGGGCTGGAA; GCCGAGGGTGCAGTTCCTGAGACACAGACTTTGACCGGATGGAGTAAGGGGTTCATTGGC; ATGCACAGGGAAATGCAAGTCAACCCCATTTCAAAGCGGATGGGGCCCATGACTGTGGTC; AGGATGGACGCTTCAGTCCAGCCAGGCCCTTTTCGGACCCTGCTCCAGTTTCTTTATACG; GGACAACTGGATGAAAAGGAAAAGGATTTGGTGGGCCTGGCTCAGATCGCAGAGGTCCTC; GAGATGTTCGATTTGAGGATGATGGTGGAAAACATCATGAACAAGGAAGCCTTCATGAAC; CAGGAGATTACGAAAGCCTTTCACGTAAGGAAAGCCAATCGGATAAAAGAGTGTCTCAGC; AAGGGAACGTTCTCGGACGTGACATTTAAATTGGACGATGGAGCCATCAGTGCCCACAAG; CCGCTGCTGATCTGTAGCTGTGAGTGGATGGCAGCCATGTTCGGGGGGTCATTTGTGGAA; AGTGCCAACAGTGAGGTGTATCTCCCGAACATAAACAAGATATCAATGCAAGCAGTATTG; GATTATCTCTATACCAAGCAGTTGTCTCCTAACTTGGATCTGGACCCGCTGGAATTAATT; GCCTTGGCAAACAGATTTTGCCTGCCACACTTGGTTGCACTTGCAGAACAGCATGCCGTT; CAGGAGTTGACCAAAGCCGCCACGAGTGGCGTGGGCATTGACGGAGAAGTGCTCTCTTAC; TTGGAATTGGCTCAGTTTCACAATGCCCACCAGTTGGCCGCCTGGTGTTTGCACCACATC; TGCACCAACTACAACAGTGTATGCTCCAAGTTCCGTAAGGAAATCAAATCAAAATCTGCA; GACAACCAGGAATACTTCGAGCGGCACCGCTGGCCCCCTGTGTGGTACCTGAAGGAAGAA; GATCACTACCAGCGTGTGAAAAGGGAACGAGAGAAGGAAGATATTGCACTAAATAAGCAT; CGCTCAAGACGAAAGTGGTGCTTCTGGAATTCATCTCCAGCAGTGGCCTGA; ; Clone variation with respect to NM_014836.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014836 unedited; NCCTTCCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA; GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG; CGAATTCGGCACGAGGCTCCGTTCCGCCTGGCTGCCCCGCCTGGGAGCGGGGTGGGGGCA; GGCGAAGGGGAGGGCTGCGGGTGGAAAGGGGCGAGCCCCTCTCGCCCGGGACTGGGAAGA; GCGGCCACGGCGCCGGGCGAGGCGGCAGGGCGAGGCAGTGCCATGCTGCCTTTGTGCAAA; GTTGGGCGGCGGGCGGCGCGCCGCGCGCAGGGGCCCCGCACACCCGCGACCGCGCGCAGC; CGTGGCTGCTGCTGGTGGGCGGCNNCNCCNNCCCNCCCCCCCCCTCCTCCTCCCCCCTCC; TCTTCCTTTCTTCCCCCTTTCCCTTTCCTCCTTCCCCCTCTTCTCTCCCTTTTTTCTCCC; CTCCCTCCCCCTTCCCCCCCCTTCCTTCCCTTTCCCCTCTCTTCCTTTTCCTCTTCCCCT; CCCCCCCCTCCTCCCCTCTCCCCTCCCCTTTCTTTTCCCCCCCCTTCTCTCTCCCTTCCC; CCCCCTTTCCCTCCCTCCCTCCTCCCCTTCCCCCTCCCTCCCTTTTTTTTCTTTTTTCCC; CCCCCCTTCTCCCCCCCTTCCCCCTTCTCTTCCCCCCTCCTCCCCCTCCTCCCCCCCCCC; TCCTCTTCTCTCCTCCTCCCCCCCCCCCCCCTCCCCCTCCCCCCCTTTTTCTCCTTCTCT; CCCCCCCCCCCCCCGCCTTTCTCCCTCCCTCCCCCCCCCCTCCNGTTTCCTTTGTCCCTT; TCCCTCCTTCCCTTTCCCCTCCCCTTCCCCCCTTTCCCCCCCCCCCCTTTTTTTCCCCTC; TCTCTCTCCCCGCCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_014836
• Insert Size: 4200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014836.3, NP_055651.1
• RefSeq Size: 4471 bp
• RefSeq ORF: 2091 bp
• Locus ID: 9886
• UniProt ID: O94844
• Cytogenetics: 10q21.2
• Summary: The protein encoded by this gene belongs to the Rho family of the small GTPase superfamily. It contains a GTPase domain, a proline-rich region, a tandem of 2 BTB (broad complex, tramtrack, and bric-a-brac) domains, and a conserved C-terminal region. The protein plays a role in small GTPase-mediated signal transduction and the organization of the actin filament system. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]; Transcript Variant: This variant (1) encodes the longer isoform. Variants 1-6 encode the same protein (isoform 1).