TRAP100 (MED24) (NM_014815) Human Untagged Clone

SKU SC111740

MED24 (untagged)-Human mediator complex subunit 24 (MED24), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: TRAP100
• Synonyms: ARC100; CRSP4; CRSP100; DRIP100; MED5; THRAP4; TRAP100
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014815, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAAGGTGGTCAACCTGAAGCAAGCCATTTTGCAAGCCTGGAAGGAGCGCTGGAGTGACTACCAATGGG; CAATCAACATGAAGAAATTCTTTCCTAAAGGAGCCACCTGGGATATTCTCAACCTGGCAGATGCGTTACT; AGAGCAGGCCATGATTGGACCATCCCCCAATCCTCTCATCTTGTCCTACCTGAAGTATGCCATTAGTTCC; CAGATGGTGTCCTACTCTTCTGTCCTCACAGCCATCAGTAAGTTTGATGACTTTTCTCGGGACCTGTGTG; TCCAGGCATTGCTGGACATCATGGACATGTTTTGTGACCGTCTGAGCTGTCACGGCAAAGCAGAGGAATG; CATCGGACTGTGCCGAGCCCTTCTTAGCGCCCTCCACTGGCTGCTGCGCTGCACGGCAGCCTCTGCAGAG; CGGCTGCGGGAGGGGCTGGAGGCCGGCACTCCAGCCGCTGGGGAGAAGCAGCTTGCCATGTGCCTTCAGC; GCCTGGAGAAAACCCTCAGCAGCACCAAGAACCGGGCCCTGCTGCACATCGCCAAACTAGAGGAGGCCTC; TTCTTGGACTGCCATCGAGCATTCTCTCTTGAAACTTGGAGAGATCCTGGCCAATCTCAGCAACCCGCAG; CTCCGGAGTCAGGCCGAGCAGTGTGGCACCCTCATTAGGAGCATCCCCACGATGCTGTCTGTGCATGCGG; AGCAGATGCACAAGACCGGCTTCCCCACTGTCCACGCCGTGATCCTGCTCGAGGGCACCATGAACCTGAC; AGGCGAGACGCAGTCCCTGGTGGAGCAGCTGACGATGGTGAAGCGCATGCAGCATATCCCCACCCCACTT; TTTGTCCTGGAGATCTGGAAAGCTTGCTTCGTGGGGCTCATTGAGTCTCCCGAGGGTACGGAGGAGCTCA; AGTGGACAGCTTTCACTTTCCTCAAGATTCCACAGGTTTTGGTGAAGTTGAAGAAGTACTCTCATGGAGA; CAAGGACTTCACTGAGGATGTCAACTGTGCTTTTGAGTTCCTGCTGAAGCTCACCCCCTTGTTGGACAAA; GCTGACCAGCGCTGCAACTGTGACTGTACAAACTTCCTGCTCCAAGAATGTGGCAAGCAGGGGCTTCTGT; CTGAGGCCAGCGTCAACAACCTTATGGCTAAGCGCAAAGCGGACCGAGAGCACGCACCCCAGCAGAAATC; GGGAGAGAATGCCAACATCCAGCCCAACATCCAGCTGATCCTCCGGGCGGAGCCCACTGTCACAAACATC; CTCAAGACGATGGATGCAGACCACTCTAAGTCACCGGAGGGACTGCTGGGAGTCCTGGGCCACATGCTGT; CCGGGAAGAGTCTGGACTTGCTGCTGGCTGCCGCCGCCGCCACTGGAAAGCTGAAATCCTTCGCCCGGAA; ATTCATCAATTTGAATGAATTCACAACCTATGGCAGCGAAGAAAGCACCAAACCGGCCTCCGTCCGGGCC; CTGCTGTTTGACATCTCCTTCCTCATGCTGTGCCATGTGGCCCAGACCTATGGTTCAGAGGTGATTCTGT; CCGAGTCGCGCACAGGAGCTGAGGTGCCCTTCTTCGAGACCTGGATGCAGACCTGCATGCCTGAGGAGGG; CAAGATCCTGAACCCTGACCACCCCTGCTTCCGCCCCGACTCCACCAAAGTGGAGTCCCTGGTGGCCCTG; CTCAACAACTCCTCGGAGATGAAGCTAGTGCAGATGAAGTGGCATGAGGCCTGTCTCAGCATCTCAGCCG; CCATCTTGGAAATCCTCAATGCCTGGGAGAATGGGGTCCTGGCCTTCGAGTCCATCCAGAAAATCACTGA; TAACATCAAAGGGAAGGTATGCAGTCTGGCGGTGTGTGCTGTGGCTTGGCTTGTGGCCCACGTCCGGATG; CTGGGGCTGGATGAGCGTGAGAAGTCGCTGCAGATGATCCGCCAGCTGGCAGGGCCACTGTTTAGTGAGA; ACACCCTGCAGTTCTACAATGAGAGGGTGGTGATCATGAACTCGATCCTGGAGCGCATGTGTGCCGACGT; GCTGCAGCAGACAGCCACGCAGATCAAGTTTCCCTCCACCGGGGTGGACACAATGCCCTACTGGAACCTG; CTGCCCCCCAAGCGGCCCATCAAAGAGGTGCTGACGGACATTTTTGCCAAGGTGCTGGAGAAGGGCTGGG; TGGACAGCCGCTCCATCCACATCTTTGACACCCTGCTGCACATGGGCGGCGTCTACTGGTTCTGCAACAA; CCTGATTAAGGAGCTGCTGAAGGAGACGCGGAAGGAGCACACGCTGCGGGCAGTGGAGCTGCTCTACTCC; ATCTTCTGCCTGGACATGCAGCAAGTGACCCTGGTCCTGCTGGGCCACATCCTACCTGGCCTGCTCACTG; ACTCCTCCAAGTGGCACAGCCTCATGGACCCCCCGGGCACTGCTCTTGCCAAGCTGGCCGTGTGGTGTGC; CCTCAGTTCCTACTCCTCCCACAAGGGACAGGCGTCCACCCGCCAGAAGAAGAGACACCGCGAAGACATT; GAGGATTATATCAGCCTCTTCCCCCTGGACGATGTGCAGCCTTCGAAGTTGATGCGACTGCTGAGCTCTA; ATGAGGACGATGCCAACATCCTTTCGAGCCCCACAGACCGATCCATGAGCAGCTCCCTCTCAGCCTCTCA; GCTCCACACGGTCAACATGCGGGACCCTCTGAACCGAGTCCTGGCCAACCTGTTCCTGCTCATCTCCTCC; ATCCTGGGGTCTCGCACCGCTGGCCCCCACACCCAGTTCGTGCAGTGGTTCATGGAGGAGTGTGTGGACT; GCCTGGAGCAGGGTGGCCGTGGCAGCGTCCTGCAGTTCATGCCCTTCACCACCGTGTCGGAACTGGTGAA; GGTGTCAGCCATGTCCAGCCCCAAGGTGGTTCTGGCCATCACGGACCTCAGCCTGCCCCTGGGCCGCCAG; GTGGCTGCTAAAGCCATTGCTGCACTCTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014815 unedited; GACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATTGTGCGCTCCATTTCTTTCACC; TCAAAGCTGGCCGCCACCGCTGCCACCTGCTCAGAGTGAAATAATGAAGGTGGTCAACCT; GAAGCAAGCCATTTTGCAAGCCTGGAAGGAGCGCTGGAGTGACTACCAATGGGCAATCAA; CATGAAGAAATTCTTTCCTAAAGGAGCCACCTGGGATATTCTCAACCTGGCAGATGCGTT; ACTAGAGCAGGCCATGATTGGACCATCCCCCAATCCTCTCATCTTGTCCTACCTGAAGTA; TGCCATTAGTTCCCAGATGGTGTCCTACTCTTCTGTCCTCACAGCCATCAGTAAGTTTGA; TGACTTTTCTCGGGACCTGTGTGTCCAGGCATTGCTGGACATCATGGACATGTTTTGTGA; CCGTCTGAGCTGTCACGGCAAAGCAGAGGAATGCATCGGACTGTGCCGAGCCCTTCTTAG; CGCCCTCCACTGGCTGCTGCGCTGCACGGCAGCCTCTGCAGAGCGGCTGCGGGAGGGGCT; GGAGGCCGGCACTCCAGCCGCTGGGGAGAAGCAGCTTGCCATGTGCCTTCAGCGCCTGGA; GAAAACCCTCAGCAGCACCAAGAACCGGGCCCTGCTGCACATCGCCAAACTAGAGGGAGC; CTCTTTCTGGACTGCCATCGAGCATTCTCTCTTGAAACTTGNAGAGATCCCTGCCAATCT; CAGCAACCCGCAGCTTCGNAGTCAGGCCGAGCAGTGTGGCACCCTCATTANGAGCATCCC; ACGATGCTGTCTGTGCATGCNGAGCANATGCACAAGAACCGCTTNCCCACTGTCCACGCC; GTGATCCTGCTCGAGGGCACATGAACCTGACAGCGAGACGCATCCCTTGGTGGACANCTG; ACGATGGTGAGCGCATGCANATATNCCCACCCACTTTTGTCCTGNAGACTGGAAAGCTGC; TCGTGGGCTCATGATCTCCGAGGNTACGAGAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014815 unedited; GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAAGGGAGCCACGAGGAGCT; TTATTTATACATGAACGAAGGTAGATTTCCCTCACATATTACAAAATACACACAAACACA; CACACACACACACACACACACACACACACATACATACTTTGCATCTAGAAAGTTCCTCAG; AGGTAAGACTACTCCTGGGCTGGGGCGGGCGCACACAGGGGTGACCACTGGGCTTGGGGT; CCAGGCTGCTCACTCTCCTCAGGTGCCAGCAGATGGAGAGGAAATTTTGAAAGAAGAAAC; CGGGAGACATCCTGGAGGTCAGGAGTCAGGAGCGGGGAACTGGAAGCCGCTAGAGGCTCT; TGCACCATGTGGAGCGGATGTGAGGCACGATGCCTCATCTTTCCTCACTGCTTCCCTTGG; AGGCGCCTGGGGCTGCGCCAGTCCCCAGCCCCCACTGCGGCCATGCCAAGCCCCTCAGAG; TGCAGCAATGGCTTTAGCAGCCACCTGGCGGCCCAGGGGCAGGCTGAGGTCCGTGATGGC; CAGAACCACCTTGGGGCTGGACATGGCTGACACCTTCACCAGTTCCGACACGGTGGTGAA; GGGCATGAACTGCAGGACGCTGCCACGGCCACCCTGTTCAGGCAGTCCACACACTCCTCC; ATGAACCACTGCACGAACTGGGTGTGGGGGCCAGCGGTGCGAGACCCCAGGATGGAGGAA; ATGACCAGGACCCGGTTGCCCAGGACTCGGTTCAGAGGGTCCCGCATGTTGACCGCGTGG; ACCTGAAAGGCTGACAGGGACCTGCTCATGGAACCGGCCTGGGGGGCCTCAAAAGGATGT; GGCACTCGCCCTCATAAAACCTCAAAAGCGCAACAACTTCAGAAGGTGGCCATTGGNCAA; GGGGGAAGAAGCTTAAATAACTCCCCATGCGCTTCGCGGGGTTTCTTTCTTTGGGGGGTG; G;
• Restriction Sites: NotI-NotI
• ACCN: NM_014815
• Insert Size: 3600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014815.2, NP_055630.2
• RefSeq Size: 3735 bp
• RefSeq ORF: 3735 bp
• Locus ID: 9862
• UniProt ID: O75448
• Cytogenetics: 17q21.1
• Protein Families: Druggable Genome, Transcription Factors
• Summary: This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).