OBFC1 (STN1) (NM_024928) Human Untagged Clone

SKU SC111636

OBFC1 (untagged)-Human oligonucleotide/oligosaccharide-binding fold containing 1 (OBFC1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: OBFC1
• Synonyms: AAF-44; AAF44; bA541N10.2; OBFC1; RPA-32
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_024928 edited; CAGCCAACTGCTCCTGCGCCGGGCGGGGTCGTCGCCGCCAGCGGCTCCGAGCGCCGGAAG; GGCCAGGTCGCAGGGCTCCTGGAGCTGCAGGCGGCGGGAGGGGCTACAAATGCTTGACTC; AGTGATGCAGAACCTTTCAGAGTTAGCTGGAAGCCACAGCCCTGCCTCTTGATGCAGCCT; GGATCCAGCCGGTGTGAAGAGGAGACCCCTTCCCTCTTGTGGGGTTTGGATCCTGTGTTT; CTAGCCTTTGCAAAACTCTACATCAGGGATATCCTGGACATGAAGGAGTCCCGCCAGGTG; CCAGGTGTATTTTTGTACAATGGACATCCAATAAAACAGGTAGATGTCTTGGGAACTGTC; ATTGGAGTGAGAGAAAGAGATGCTTTCTACAGTTATGGAGTGGATGACAGCACTGGAGTT; ATAAACTGCATCTGCTGGAAAAAGTTGAATACTGAGTCTGTATCAGCTGCTCCTAGTGCA; GCAAGAGAGCTCAGCTTAACCTCACAACTTAAGAAGCTACAAGAGACCATTGAGCAGAAA; ACAAAGATAGAGATCGGGGACACGATCCGAGTCAGAGGCAGTATCCGCACATACAGAGAA; GAGCGAGAGATTCATGCCACCGCTTACTATAAAGTGGACGACCCAGTGTGGAACATTCAA; ATTGCAAGGATGCTTGAGCTGCCCACTATCTACAGGAAAGTTTATGACCAGCCTTTTCAC; AGCTCAGCCCTAGAGAAAGAAGAGGCACTAAGCAATCCAGGCGCCCTGGACCTCCCCAGT; CTCACGAGTTTGCTGAGTGAAAAAGCCAAAGAATTCCTCATGGAGAACAGAGTGCAGAGC; TTTTACCAGCAGGAGCTGGAAATGGTGGAGTCTTTGCTGTCCCTTGCCAATCAGCCTGTG; ATTCACAGTGCCTGCTCCGACCAAGTGAATTTTAAGAAGGACACCACTTCCAAGGCAATT; CATAGTATATTTAAGAATGCTATACAACTGCTGCAGGAAAAAGGACTTGTTTTCCAGAAA; GATGATGGTTTTGATAACCTATACTATGTAACCAGAGAAGACAAAGACCTGCACAGAAAG; ATCCACCGGATCATTCAGCAGGACTGCCAGAAACCAAATCACATGGAGAAGGGCTGTCAC; TTCCTGCACATCTTGGCCTGTGCTCGCCTGAGCATCCGCCCGGGCCTGAGCGAGGCTGTG; CTGCAGCAAGTTCTGGAGCTCCTGGAGGACCAGAGTGACATTGTCAGCACAATGGAGCAC; TACTACACAGCGTTCTGAGCAGAGACACGCAGACCAGCTGAGGAGGACAAAGATAAGGTG; GCATTCACCCCCAGGCTCTGACTTTCAGCATCATGCAGGGGCTTATCTGTCTGGAGGCAG; TTACCTCATAATAAACTATAAAATATAGTCATCTTGGGAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024928 unedited; AACTCATAANTGTAAACGACTTACTATAGGCGGCCGCGAATTCGCACGAGGCAGCCAACT; GCTCCTGCGCCGGGCGGGGTCGTCGCCGCCAGCGGCTCCGAGCGCCGGAAGGGCCAGGTC; GCAGGGCTCCTGGAGCTGCAGGCGGCGGGAGGGGCTACAAATGCTTGACTCAGTGATGCA; GAACCTTTCAGAGTTAGCTGGAAGCCACAGCCCTGCCTCTTGATGCAGCCTGGATCCAGC; CGGTGTGAAGAGGAGACCCCTTCCCTCTTGTGGGGTTTGGATCCTGTGTTTCTAGCCTTT; GCAAAACTCTACATCAGGGATATCCTGGACATGAAGGAGTCCCGCCAGGTGCCAGGTGTA; TTTTTGTACAATGGACATCCAATAAAACAGGTAGATGTCTTGGGAACTGTCATTGGAGTG; AGAGAAAGAGATGCTTTCTACAGTTATGGAGTGGATGACAGCACTGGAGTTATAAACTGC; ATCTGCTGGAAAAAGTTGAATACTGAGTCTGTATCAGCTGCTCCTAGTGCAGCAAGAGAG; CTCAGCTTAACCTCACAACTTAAGAAGCTACAAGAGACCATTGAGCAGAAAACAAAGATA; GAGATCGGGGACACGATCCGAGTCAGAGGCAGTATCCGCACATACAGAGAAGAGCGAGAG; ATTCATGCCACCGCTTACTATAAAGTGGACGACCCAGTGTGGAACATTCAAATTGCAAGG; ATGCTTGAGCTGCCCACTATCTACAGGGAAGTTTATGACCAGCCTTTTCACAGCTCAGCC; CTAGAGAAAGAAGAGCACTAAGCAATCCAGGCGCCCTGGACCTNCCCAGTCTCACGAGTT; TGCTGAGTGAAAAGCCAAAGATTCCTCATGAGAACAGAGTGCAGAGCTTTTACAGCAGAG; CTGGAATGGNNGNAGTCTTGCTGTCCTTTGCAATCAGCCTGTGATCACAGTGCCG;
• Restriction Sites: Please inquire
• ACCN: NM_024928
• Insert Size: 1460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_024928.3, NP_079204.1
• RefSeq Size: 1451 bp
• RefSeq ORF: 1107 bp
• Locus ID: 79991
• UniProt ID: Q9H668
• Cytogenetics: 10q24.33
• Summary: OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).[supplied by OMIM, Nov 2009]