Amyloid Precursor Protein (APP) (NM_000484) Human Untagged Clone

SKU SC111589

APP (untagged)-Human amyloid beta (A4) precursor protein (APP), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Amyloid Precursor Protein
• Synonyms: AAA; ABETA; ABPP; AD1; alpha-sAPP; APPI; CTFgamma; CVAP; PN-II; PN2; preA4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000484 edited; CTCTGAGCCCCGCCGCCGCGCTCGGGCTCCGTCAGTTTCCTCGGCAGCGGTAGGCGAGAG; CACGCGGAGGAGCGTGCGCGGGGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAG; CAAGGACGCGGCGGATCCCACTCGCACAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCG; ATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGAGGTA; CCCACTGATGGTAATGCTGGCCTGCTGGCTGAACCCCAGATTGCCATGTTCTGTGGCAGA; CTGAACATGCACATGAATGTCCAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAA; ACCTGCATTGATACCAAGGAAGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTG; CAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGG; GGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGT; GAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGG; ATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAG; AAGAGTACCAACTTGCATGACTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGA; GGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGAT; GCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGG; AGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAA; GAAGCCGATGATGACGAGGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAA; CCCTACGAAGAAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCACCACCACCACA; GAGTCTGTGGAAGAGGTGGTTCGAGAGGTGTGCTCTGAACAAGCCGAGACGGGGCCGTGC; CGAGCAATGATCTCCCGCTGGTACTTTGATGTGACTGAAGGGAAGTGTGCCCCATTCTTT; TACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAGAAGAGTACTGCATGGCCGTG; TGTGGCAGCGCCATGTCCCAAAGTTTACTCAAGACTACCCAGGAACCTCTTGCCCGAGAT; CCTGTTAAACTTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAG; ACACCTGGGGATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCC; AAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGGCAGAACGTCAAGCA; AAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGTGGAA; TCTTTGGAACAGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGA; GTGGAAGCCATGCTCAATGACCGCCGCCGCCTGGCCCTGGAGAACTACATCACCGCTCTG; CAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCGCA; GAACAGAAGGACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCC; AAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTGTGATTTATGAGCGC; ATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGGAT; GAAGTTGATGAGCTGCTTCAGAAAGAGCAAAACTATTCAGATGACGTCTTGGCCAACATG; ATTAGTGAACCAAGGATCAGTTACGGAAACGATGCTCTCATGCCATCTTTGACCGAAACG; AAAACCACCGTGGAGCTCCTTCCCGTGAATGGAGAGTTCAGCCTGGACGATCTCCAGCCG; TGGCATTCTTTTGGGGCTGACTCTGTGCCAGCCAACACAGAAAACGAAGTTGAGCCTGTT; GATGCCCGCCCTGCTGCCGACCGAGGACTGACCACTCGACCAGGTTCTGGGTTGACAAAT; ATCAAGACGGAGGAGATCTCTGAAGTGAAGATGGATGCAGAATTCCGACATGACTCAGGA; TATGAAGTTCATCATCAAAAATTGGTGTTCTTTGCAGAAGATGTGGGTTCAAACAAAGGT; GCAATCATTGGACTCATGGTGGGCGGTGTTGTCATAGCGACAGTGATCGTCATCACCTTG; GTGATGCTGAAGAAGAAACAGTACACATCCATTCATCATGGTGTGGTGGAGGTTGACGCC; GCTGTCACCCCAGAGGAGCGCCACCTGTCCAAGATGCAGCAGAACGGCTACGAAAATCCA; ACCTACAAGTTCTTTGAGCAGATGCAGAACTAGACCCCCGCCACAGCAGCCTCTGAAGTT; GGACAGCAAAACCATTGCTTCACTACCCATCGGTGTCCATTTATAGAATAATGTGGGAAG; AAACAAACCCGTTTTATGATTTACTCATTATCGCCTTTTGACAGCTGTGCTGTAACACAA; GTAGATGCCTGAACTTGAATTAATCCACACATCAGTAATGTATTCTATCTCTCTTTACAT; TTTGGTCTCTATACTACATTATTAATGGGTTTTGTGTACTGTAAAGAATTTAGCTGTATC; AAACTAGTGCATGAATAGATTCTCTCCTGATTATTTATCACATAGCCCCTTAGCCAGTTG; TATATTATTCTTGTGGTTTGTGACCCAATTAAGTCCTACTTTACATATGCTTTAAGAATC; GATGGGGGATGCTTCATGTGAACGTGGGAGTTCAGCTGCTTCTCTTGCCTAAGTATTCCT; TTCCTGATCACTATGCATTTTAAAGTTAAACATTTTTAAGTATTTCAGATGCTTTAGAGA; GATTTTTTTTCCATGACTGCATTTTACTGTACAGATTGCTGCTTCTGCTATATTTGTGAT; ATAGGAATTAAGAGGATACACACGTTTGTTTCTTCGTGCCTGTTTTATGTGCACACATTA; GGCATTGAGACTTCAAGCTTTTCTTTTTTTGTCCACGTATCTTTGGGTCTTTGATAAAGA; AAAGAATCCCTGTTCATTGTAAGCACTTTTACGGGGCGGGTGGGGAGGGGTGCTCTGCTG; GTCTTCAATTACCAAGAATTCTCCAAAACAATTTTCTGCAGGATGATTGTACAGAATCAT; TGCTTATGACATGATCGCTTTCTACACTGTATTACATAAATAAATTAAATAAAATAAAAA; AAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000484 unedited; TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCTGAGCCCCGCCGCCGCGC; TCGGGCTCCGTCAGTTTCCTCGGCAGCGGTAGGCGAGAGCACGCGGAGGAGCGTGCGCGG; GGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAGCAAGGACGCGGCGGATCCCAC; TCGCACAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCGATGCTGCCCGGTTTGGCACTG; CTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGAGGTACCCACTGATGGTAATGCTGGC; CTGCTGGCTGAACCCCAGATTGCCATGTTCTGTGGCAGACTGAACATGCACATGAATGTC; CAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAAACCTGCATTGATACCAAGGAA; GGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAA; GCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGGNGCCGCAAGCAGTGCAAGACC; CATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTT; CTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGGATGGGATGTNTNGCGAAACTC; ATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTTGCATG; ACTACGGCATGTTGCTGCCCTGCGGNATTGACAGTNCCGAGGTGACAGTTCTGGGCGCCT; GCCCCTGCCTGNAGAAAGCGACATGCGGCTCCCGCTGTGCGGCACGAGATGCCCACGCAG; CCTGCCGCCCCGTCCTACTCTCCCTTACCCCGTTCTCTCCCCCTCCCCCCCCCTCCTCCC; GCCCCCCCCCCGTCTTTATTACGCGTCCTCCTCTCTCCCTGCATCGTCGTTCCTGGCTGT; ACCCCCCCATCTCACCCACTTCGGCCTTCCTCCG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000484 unedited; CCATCGNGANNGATGGCAACTTCCCAGGTTCAGGAAAGCACTGGGGCAGGGTCACAGGGC; TGCCACCCGGGTTCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGT; TTTTTTTTTTTTTTTTTATTTTATTTAATTTATTTATGTAATACAGTGTAGAAAGCGATC; ATGTCATAAGCAATGATTCTGTACAATCATCCTGCAAAAAATTGTTTTGGAGAATTCTTG; GTAATTGAAGACCAGCAGAGCACCCCTCCCCACCCGCCCCGTAAAAGTGCTTACAATGAA; CAGGGATTCTTTTCTTTATCAAAGACCCAAAGATACGTGGACAAAAAAAGAAAAGCTTGA; AGTCTCAATGCCTAATGTGTGCACATAAAACAGGCACGAAGAAACAAACGTGTGTATCCT; CTTAATTCCTATATCACAAATATAGCAGAAGCAGCAATCTGTACAGTAAAATGCAGTCAT; GGAAAAAAAATCTCTCTAAAGCATCTGAAATACTTAAAAATGTTTAACTTTAAAATGCAT; AGTGATCAGGAAAGGAATACTTAGGCAAGAGAAGCAGCTGAACTCCCACGTTCACATGAA; GCATCCCCCATCGATTCTTAAAGCATATGTAAAGTAAGACTTAATTGGGTCACACACCAC; AAGAATAATATACAACTGGCTAAGGGGCTATGTGATAAATAATCAGGAAAGAATCCTTTC; CTGCCCTAGTTTGATACAGCTAAATTCTTTACGGTCACCAAACCCTTTTTTTATTGTTTT; TTGAGCCCAATTGTAAGGGGGGGTGGAATCCCTTCCGGGTGTGTGGTTCCATTCAGGGTT; AGCTTTTTTTTGTTTTCACTCCCTCTGCCCATGGCGNTTTTGTGTAACCCTAAAAAGGGC; GTTTTCTTCCCCCTTTTTCTTTGAGGGCCCAAAGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_000484
• Insert Size: 3600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_000484.2.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000484.2, NP_000475.1
• RefSeq Size: 3641 bp
• RefSeq ORF: 2313 bp
• Locus ID: 351
• UniProt ID: P05067
• Cytogenetics: 21q21.3
• Domains: A4_EXTRA, Beta-APP, KU
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Alzheimer's disease
• Summary: This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a, also known as PreA4 770).