NLN (NM_020726) Human Untagged Clone

SKU SC111375

NLN (untagged)-Human neurolysin (metallopeptidase M3 family) (NLN), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: NLN
• Synonyms: AGTBP; EP24.16; MEP; MOP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020726, the custom clone sequence may differ by one or more nucleotides; ; ; ATGATCGCCCGGTGCCTTTTGGCTGTGCGAAGCCTCCGCAGAGTTGGTGGTTCCAGGATTTTACTCAGAA; TGACGTTAGGAAGAGAAGTGATGTCTCCTCTTCAGGCAATGTCTTCCTATACTGTGGCTGGCAGAAATGT; TTTAAGATGGGATCTTTCACCAGAGCAAATTAAAACAAGAACTGAGGAGCTCATTGTGCAGACCAAACAG; GTGTACGATGCTGTTGGAATGCTCGGTATTGAGGAAGTAACTTACGAGAACTGTCTGCAGGCACTGGCAG; ATGTAGAAGTAAAGTATATAGTGGAAAGGACCATGCTAGACTTTCCCCAGCATGTATCCTCTGACAAAGA; AGTACGAGCAGCAAGTACAGAAGCAGACAAAAGACTTTCTCGTTTTGATATTGAGATGAGCATGAGAGGA; GATATATTTGAGAGAATTGTTCATTTACAGGAAACCTGTGATCTGGGGAAGATAAAACCTGAGGCCAGAC; GATACTTGGAAAAGTCAATTAAAATGGGGAAAAGAAATGGGCTCCATCTTCCTGAACAAGTACAGAATGA; AATCAAATCAATGAAGAAAAGAATGAGTGAGCTATGTATTGATTTTAACAAAAACCTCAATGAGGATGAT; ACCTTCCTTGTATTTTCCAAGGCTGAACTTGGTGCTCTTCCTGATGATTTCATTGACAGTTTAGAAAAGA; CAGATGATGACAAGTATAAAATTACCTTAAAATATCCACACTATTTCCCTGTCATGAAGAAATGTTGTAT; CCCTGAAACCAGAAGAAGGATGGAAATGGCTTTTAATACAAGGTGCAAAGAGGAAAACACCATAATTTTG; CAGCAGCTACTCCCACTGCGAACCAAGGTGGCCAAACTACTCGGTTATAGCACACATGCTGACTTCGTCC; TTGAAATGAACACTGCAAAGAGCACAAGCCGCGTAACAGCCTTTCTAGATGATTTAAGCCAGAAGTTAAA; ACCCTTGGGTGAAGCAGAACGAGAGTTTATTTTGAATTTGAAGAAAAAGGAATGCAAAGACAGGGGTTTT; GAATATGATGGGAAAATCAATGCCTGGGATCTATATTACTACATGACTCAGACAGAGGAACTCAAGTATT; CCATAGACCAAGAGTTCCTCAAGGAATACTTCCCAATTGAGGTGGTCACTGAAGGCTTGCTGAACACCTA; CCAGGAGTTGTTGGGACTTTCATTTGAACAAATGACAGATGCTCATGTTTGGAACAAGAGTGTTACACTT; TATACTGTGAAGGATAAAGCTACAGGAGAAGTATTGGGACAGTTCTATTTGGACCTCTATCCAAGGGAAG; GAAAATACAATCATGCGGCCTGCTTCGGTCTCCAGCCTGGCTGCCTTCTGCCTGATGGAAGCCGGATGAT; GGCAGTGGCTGCCCTCGTGGTGAACTTCTCACAGCCAGTGGCAGGTCGTCCCTCTCTCCTGAGACACGAC; GAGGTGAGGACTTACTTTCATGAGTTTGGTCACGTGATGCATCAGATTTGTGCACAGACTGATTTTGCAC; GATTTAGCGGAACAAATGTGGAAACTGACTTTGTAGAGGTGCCATCGCAAATGCTTGAAAATTGGGTGTG; GGACGTCGATTCCCTCCGAAGATTGTCAAAACATTATAAAGATGGAAGCCCTATTGCAGACGATCTGCTT; GAAAAACTTGTTGCTTCTAGGCTGGTCAACACAGGTCTTCTGACCCTGCGCCAGATTGTTTTGAGCAAAG; TTGATCAGTCTCTTCATACCAACACATCGCTGGATGCTGCAAGTGAATATGCCAAATACTGCTCAGAAAT; ATTAGGAGTTGCAGCTACTCCAGGCACAAATATGCCAGCTACCTTTGGACATTTGGCAGGGGGATACGAT; GGCCAATATTATGGATATCTTTGGAGTGAAGTATTTTCCATGGATATGTTTTACAGCTGTTTTAAAAAAG; AAGGGATAATGAATCCAGAGGTTGGAATGAAATACAGAAACCTAATCCTGAAACCTGGGGGATCTCTGGA; CGGCATGGACATGCTCCACAATTTCTTGAAACGTGAGCCAAACCAAAAAGCGTTCCTAATGAGTAGAGGC; CTGCATGCTCCGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020726 unedited; CCCCCCCGGGGNNNCCNNCNCNNCCNNNCCNCTTTCTCCNNNCCCCCCCNNCCCGTTCAA; ATTTGTNATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGGCGTGGAGCTGCCG; CACGTGGGAGGGCGCTGGCCAGGCAGCCACTGTGGCCTCTGCGGCTAGGCCGGCTCGAGA; CTCCCGGGCGCCCAGGCGCTGCCGCCCGCCTCGCCGCCCCACGCCGAAGGACCACGCGCC; CGCCGCCGCCAGCCTCTCAGCGCTCCCATGATCGCCCGGTGCCTTTTGGCTGTGCGAAGC; CTCCGCAGAGTTGGTGGTTCCAGGATTTTACTCAGAATGACGTTAGGAAGAGAAGTGATG; TCTCCTCTTCAGGCAATGTCTTCCTATACTGTGGCTGGCAGAAATGTTTTAAGATGGGAT; CTTTCACCAGAGCAAATTAAAACAAGAACTGAGGAGCTCATTGTGCAGACCAAACAGGTG; TACGATGCTGTTGGAATGCTCGGTATTGAGGAAGTAACTTACGAGAACTGTCTGCAGGCA; CTGGCAGATGTAGAAGTAAAGTATATAGTGGAAAGGACCATGCTAGACTTTCCCCAGCAT; GTATCCTCTGACAAAGAAGTACGAGCAGCAAGTACAGAAGCAGACAAAAGACTTTCTCGT; TNTGATATTGAGATGAGCATGAGAGGAGATATATTTGAGAGAATTGTTCATTTACAGGAN; ACCTGTGATCTGGGGAAGATAAAACCTGGAGCCAGACGATACTTGGAAAAGTCAATTAAA; ATGGGGAAAAGAAATGGGCTCCATCTTTCTGAAACAGTACAGAATGAAATCAAATCAATG; AAGACAGAATGAGTGAGCTATGTATTGATTTTACCAAAACCCCTCATGAGATGATACCTT; CCTTGTATTTTCCAGGNCTGAACTTGGNGCTCTCCCTGAGATTTCATTGCCAGGTTAAAA; AGACGATGATGACAGGTTAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020726 unedited; NTTTAGCTTGGACCGGCGGCCGCAATCTANGGATCGGTTTTTTTTTTTTTTTTTTGGGAT; GATTCATTTATCAGTCCAGAAATCCCAAGTACAAAACTTCAAGATACAAGAAGGATCAAA; TTATATCATATATATGATTCAATTTAAAAATTCTTAGCCCTCTTATATCATATTATCTAG; ATTATAATAGTAAAAAATCAAGTTACATTCATATGAAACTTTCATAAAAAGAAATCAAAT; CCAGTTTTATGAAATTTTATAGTACAATTACTTTCTAGTGGGTCTTTTCTTAGGTCACAG; TATTTATAATTCCATTTACATCTTTATAATTTTTAAAATTAGAAAACAAAAGGATGTCAA; TAGAAATCTAAATTTTCACTTGCAAAACTCCCTTCAGTTTCCAGGCCAGTAACACATGGT; GATGTCGACTTGTCCTCCAGACATGGACGGCTACCAAAGATCCCCAGTTCACGGAGCATG; CAGGCCTCTACTCATTAGGAACGCTTTTTGGTTTGGCTCACGTTTCAAGAAATTGTGGAG; CATGTCCATGCCGTCCAGAGATCCCCCAGGTTTCAGGATTAGGTTTCTGTATTTCATTCC; AACCTCCGGATTCATTATCCCTTCTTTTTTAAAACAGCTGTAAAACATATCCATGGAAAA; TACTTCACTCCAAAGATATCCATAATATTGGCCATCGTATCCCCCTGCCAAATGTCCAAA; GGTAGCTGGCATATTTGTGCCTGGAGTAGCTGCAACTCCTAATATTTCTGAGCAGTATTT; GGCATATTCACTTGCAGCATCCAGCGATGTGTTGGTATGAAGAGACTGATCAACTTTGCT; CAAACAATCTGGCGCAGGTCAGAAGACCTGTGTTGAGCAGCCTAGAAGCCACAGTTTTCA; AGCAGATCGTCTGCATAGGGCTTNCTCCTTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_020726
• Insert Size: 2720 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020726.2, NP_065777.1
• RefSeq Size: 2882 bp
• RefSeq ORF: 2115 bp
• Locus ID: 57486
• UniProt ID: Q9BYT8
• Cytogenetics: 5q12.3
• Domains: Peptidase_M3
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Renin-angiotensin system
• Summary: This gene encodes a member of the metallopeptidase M3 protein family that cleaves neurotensin at the Pro10-Tyr11 bond, leading to the formation of neurotensin(1-10) and neurotensin(11-13). The encoded protein is likely involved in the termination of the neurotensinergic signal in the central nervous system and in the gastrointestinal tract.[provided by RefSeq, Jun 2010]