VCIP 135 (VCPIP1) (NM_025054) Human Untagged Clone

SKU SC111292

VCPIP1 (untagged)-Human valosin containing protein (p97)/p47 complex interacting protein 1 (VCPIP1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: VCIP 135
• Synonyms: DUBA3; VCIP135
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_025054 edited; GAATTCGGCACGAGCTGATGTCCATCAGTATTTCTACAGAAGGACTGGAGTGATAGGAGT; TCAGCCTGAGGAAGTCACAGCAGCTGCTAAAAAAGCAGTAATGGATAATCGCCTTCACAA; ATGTTTGCTCTGTGGTGCCCTTTCTGAACTTCATGTTCCTCCAGAGTGGTTGGCTCCTGG; AGGGAAATTGTATAACCTGGCAAAAAGTACTCATGGACAGCTGAGGACTGACAAAAATTA; CAGCTTTCCCTTGAACAATTTGGTTTGCTCATATGATTCAGTGAAAGATGTTCTGGTACC; AGACTATGGAATGAGTAACCTAACAGCTTGTAATTGGTGCCATGGCACATCTGTGCGAAA; GGTCAGAGGAGATGGGTCTATTGTGTATTTGGATGGAGACAGAACTAATTCTAGGTCCAC; TGGTGGCAAATGTGGTTGTGGATTCAAACACTTTTGGGATGGTAAGGAGTATGACAATCT; ACCAGAAGCTTTCCCTATTACTTTAGAATGGGGTGGAAGAGTGGTCAGAGAAACAGTATA; TTGGTTCCAGTATGAAAGTGATTCATCTTTGAATAGTAATGTTTACGATGTTGCAATGAA; ACTTGTTACCAAGCACTTTCCAGGTGAATTTGGGAGTGAAATCCTAGTTCAGAAAGTTGT; CCACACTATATTGCATCAGACTGCCAAAAAGAATCCCGATGATTATACTCCTGTAAATAT; AGATGGTGCTCACGCCCAAAGAGTTGGAGATGTTCAAGGACAAGAATCAGAGTCTCAGCT; CCCAACTAAAATTATTCTTACTGGACAGAAAACAAAAACTTTGCACAAGGAGGAGTTAAA; CATGAGTAAAACTGAAAGAACTATTCAACAGAATATTACGGAACAGGCTTCTGTAATGCA; GAAACGGAAAACAGAGAAGTTAAAACAAGAACAAAAAGGGCAACCCAGGACTGTTTCTCC; CAGTACCATTCGTGATGGTCCATCCTCTGCACCTGCTACACCTACCAAGGCTCCCTATTC; ACCGACAACTTCTAAGGAGAAGAAGATCCGAATCACAACTAATGATGGACGACAGTCCAT; GGTTACCCTTAAGTCTTCAACAACCTTTTTTGAACTTCAGGAAAGTATAGCCAGAGAATT; CAACATTCCTCCATATTTACAGTGTATTCGATACGGGTTTCCTCCTAAAGAGTTAATGCC; ACCACAGGCAGGAATGGAAAAGGAACCAGTTCCTTTACAGCATGGCGACAGAATTACAAT; AGAAATTCTAAAAAGTAAAGCTGAAGGTGGTCAGTCTGCTGCAGCACACTCAGCCCACAC; TGTGAAACAAGAAGATATTGCTGTTACTGGTAAACTGTCATCTAAGGAACTTCAGGAGCA; AGCTGAAAAAGAAATGTACTCCTTGTGTCTTTTAGCAACATTAATGGGAGAAGATGTATG; GTCTTATGCAAAGGGACTTCCTCACATGTTTCAGCAGGGTGGTGTATTCTACAGTATTAT; GAAGAAAACCATGGGTATGGCTGATGGCAAGCATTGTACTTTTCCACATCTGCCTGGCAA; AACCTTTGTCTATAATGCTTCTGAAGATAGACTGGAATTGTGTGTGGATGCTGCAGGACA; TTTCCCCATTGGTCCTGATGTTGAAGATTTAGTTAAAGAGGCTGTAAGTCAGGTTCGAGC; AGAGGCTACTACAAGAAGTAGGGAATCAAGTCCCTCACATGGGCTATTAAAACTAGGTAG; TGGTGGAGTAGTGAAAAAGAAATCTGAGCAACTTCATAACGTAACTGCCTTTCAGGGAAA; AGGGCATTCTTTAGGAACTGCATCTGGTAACCCACACCTTGATCCAAGAGCTAGGGAAAC; TTCAGTTGTAAGAAAGCATAATACAGGGACAGACTTTAGTAATAGTTCCACTAAAACAGA; GCCTTCTGTATTCACAGCTTCTTCTAGTAATAGTGAGCTTATTCGAATAGCTCCTGGAGT; AGTAACAATGAGAGACGGCAGGCAGCTTGATCCTGATTTGGTTGAGGCCCAGCGAAAAAA; ATTGCAGGAAATGGTTTCTTCTATTCAGGCTTCAATGGACAGGCACCTTCGGGATCAAAG; TACAGAGCAGTCACCATCTGATCTTCCTCAAAGGAAAACAGAAGTTGTGAGTTCTTCTGC; AAAGTCTGGGAGTCTTCAGACTGGTTTGCCTGAATCTTTTCCTTTAACTGGTGGTACTGA; AAATTTGAATACAGAAACAACTGATGGCTGTGTAGCAGATGCACTGGGAGCAGCCTTTGC; CACAAGGTCAAAAGCACAAAGGGGAAATTCCGTGGAGGAGCTTGAAGAGATGGATAGTCA; AGATGCTGAGATGACTAACACAACTGAGCCAATGGATCACTCTTGATTTAATTAGAGGCT; AATAAAGGCAGAATGTTTATTGTGAATATGTAATATTTGTTGGCTGGGCCACGTAACTTG; ATTAGTCATTAAAAATCTTGTACGTATATAATTCAAAGATTATATCTTGTTATTCAGTGC; ATGATAGCAAGTGTGTGATTGGCCATAGCTTTTAATATACTGCTGCCCAGGCTGGCTCTG; AATTTCTATAAATTAGCTGTTAGATCTGCTGAGATGACTTTCTTCCATATATGTGGTTCA; TTGGAAGTTCTTTGTAATTTTAATTCCATGAAAGTCTTAATGTTTCCAACATGAAAATTC; TCTTACTTAAATACGTTTGATTTCTGAAAAGCCTACAAATGGAGGGGAAATGAGATTGTG; CTAATGTTGATGTCATCAAGGTAACCATCTCATATACTAGTGTCAAAAGGTTTATGTAAA; CTTGAATTTTATCTGAACTAAATTGTATCTTAAAATTCATACAATTATTTATTAGACAGT; GGAATGCAAATGTTTCTATGAACGTAAACACATTCATAGAGATTTCTTGGCGAAAATATC; TTGAGAATAGAATGTAACGATATAACATTTTGCTGATTTGTGCAAGATTGGGGAAAAATT; TCTTCTAGATTGATGAGGTCAATGTGATTCAGTTGTGTTGCTGTCTGAGCCACAGCCAAT; TGTGTGTGCTCTATTAATGTTACCATTATTTTTGATTCCTGGCTTGGTCAAGAACAACCT; GATTTTTTTTTTTTTAATCTTGCAGAAAAGTTGATTGCAAAGTGTGGTAGAAAAAGAAAT; AATGGTAGCAGTAGAGAATTTATAAATAAGAGTTTATCTTTATGAATGAGTTAATTGTTC; TACAATTTTGATCAGTGGTATTTAAGCCTGTATATGCTAAGTGTATTTATGCCCCTTTGA; GCCAAAATTTTTTTTTTTTTTTTCAGAATCCATAAGAGTTTATTTGTTGGCTTCACTTGG; GTTTAGATGCAAATTTTACTGCATCACACCCTGATCAGCTGGGTATTTGCTTAGTGTATT; CATCAAATCTTCAGTGCCAGTTTCCTTGAGAAGCAGCTTTCATGCTTATTCCTTAAAAAC; AACAGAAGAGTGTTTATCCTGAGATTGGTATTGCACTATTTTATTTTATTCTGCAAATCT; GTATCCTGAAGTGTACATTTCAACCCATATTCTTCTCTGTTCACTGAAATTAAGTTGAAT; TTGAATGGCTGAAAGACAAAATATATGTTAATACAATCTCTTACCTAGAAAAAAAAAAAA; AAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025054 unedited; GGCGGCCCGCGAATTCGCACGAGGCTGATGTCCATCAGTATTTCTACAGAAGGACTGGAG; TGATAGGAGTTCAGCCTGAGGAAGTCACAGCAGCTGCTAAAAAAGCAGTAATGGATAATC; GCCTTCACAAATGTTTGCTCTGTGGTGCCCTTTCTGAACTTCATGTTCCTCCAGAGTGGT; TGGCTCCTGGAGGGAAATTGTATAACCTGGCAAAAAGTACTCATGGACAGCTGAGGACTG; ACAAAAATTACAGCTTTCCCTTGAACAATTTGGTTTGCTCATATGATTCAGTGAAAGATG; TTCTGGTACCAGACTATGGAATGAGTAACCTAACAGCTTGTAATTGGTGCCATGGCACAT; CTGTGCGAAAGGTCAGAGGAGATGGGTCTATTGTGTATTTGGATGGAGACAGAACTAATT; CTAGGTCCACTGGTGGCAAATGTGGTTGTGGATTCAAACACTTTTGGGATGGTAAGGAGT; ATGACAATCTACCAGAAGCTTTCCCTATTACTTTAGAATGGGGTGGAAGAGTGGTCAGAG; AAACAGTATATTGGTTCCAGTATGAAAGTGATTCATCTTTGAATAGTAATGTTTACGATG; TTGCAATGAAACTTGTTACCAAGCACTTTCCAGGTGAATTTGGGAGTGAAATCCTAGTTC; AGAAAGTTGTCCACACTATATTGCATCAGACTGCCAAAAAGAATCCCGATGATTATACTC; CTGTAATATAGATGGTGCTCACGCCCAAAGAGTGGNAGATGTTCAAGGACCAGAATCAGA; GTCTCAGCTCCCACTAAAATTATTCTTACTGGACAGAANACAAAAACTTGCACAGGNAGA; GTTAACATGAGTAAACTGAAAGACTATC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_025054 unedited; TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTCT; AGGTAAGAGATTGTATTAACATATATTTTGTCTTTCAGCCATTCAAATTCAACTTAATTT; CAGTGAACAGAGAAGAATATGGGTTGAAATGTACACTTCAGGATACAGATTTGCAGAATA; AAATAAAATAGTGCAATACCAATCTCAGGATAAACACTCTTCTGTTGTTTTTAAGGAATA; AGCATGAAAGCTGCTTCTCAAGGAAACTGGCACTGAAGATTTGATGAATACACTAAGCAA; ATACCCAGCTGATCAGGGTGTGATGCAGTAAAATTTGCATCTAAACCCAAGTGAAGCCAA; CAAATAAACTCTTATGGATTCTGAAAAAAAAAAAAAAAATTTTGGCTCAAAGGGGCATAA; ATACACTTAGCATATACAGGCTTAAATACCACTGATCAAAATTGTAGAACAATTAACTCA; TTCATAAAGATAAACTCTTATTTATAAATTCTCTACTGCTACCATTATTTCTTTTTCTAC; CACACTTTGCAATCAACTTTTCTGCAAGATTAAAAAAAAAAAAATCAGGTTGTTCTTGAC; CAAGCCAGGAATCAAAAATAATGGTAACATTAATAGAGCACACACAATTGGCTGTGGCTC; AGACAGCAACACAACTGAATCACATTGACCTCATCAATCTAGAAGAAATTTTTCCCCAAT; CTTGCACAAATCAGCAAAATGTTATATCGTTACATTCTATTCTCAAGAATTTTCGCCAAG; AAATCTCTATGAATGTGTTTACGTTCATAAAACATTTGCATTCCACTGTCTAATAAAAAT; TGTATGAATTTTAAGAACATTTAGTTCAGATAAATTCAGTTTACATAAACCTTTTGACCT; TGTATATGAAATGTTACCNTGATGACATCACATAGCACATCTCATTTCCCTCCATTGTAG; GGCTTTCAGAATCAACGTATTTAGTTAGAGAATTTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_025054
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_025054.3, NP_079330.2
• RefSeq Size: 8192 bp
• RefSeq ORF: 3669 bp
• Locus ID: 80124
• UniProt ID: Q96JH7
• Cytogenetics: 8q13.1
• Summary: Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP (By similarity). Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains.[UniProtKB/Swiss-Prot Function]