VPS24 (CHMP3) (NM_016079) Human Untagged Clone

CAT#: SC111287

CHMP3 (untagged)-Human vacuolar protein sorting 24 homolog (S. cerevisiae) (VPS24), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VPS24
• Synonyms: CGI-149; NEDF; VPS24
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111287 sequence for NM_016079 edited (data generated by NextGen Sequencing)
  ATGGGGCTGTTTGGAAAGACCCAGGAGAAGCCGCCCAAAGAACTGGTCAATGAGTGGTCA
  TTGAAGATAAGAAAGGAAATGAGAGTTGTTGACAGGCAAATAAGGGATATCCAAAGAGAA
  GAAGAAAAAGTGAAACGATCTGTGAAAGATGCTGCCAAGAAGGGCCAGAAGGATGTCTGC
  ATAGTTCTGGCCAAGGAGATGATCAGGTCAAGGAAGGCTGTGAGCAAGCTGTATGCATCC
  AAAGCCCACATGAACTCAGTGCTCATGGGGATGAAGAACCAGCTCGCGGTCTTGCGAGTG
  GCTGGTTCCCTGCAGAAGAGCACAGAAGTGATGAAGGCCATGCAAAGTCTTGTGAAGATT
  CCAGAGATTCAGGCCACCATGAGGGAGTTGTCCAAAGAAATGATGAAGGCTGGGATCATA
  GAGGAGATGTTAGAGGACACTTTTGAAAGCATGGACGATCAGGAAGAAATGGAGGAAGAA
  GCAGAAATGGAAATTGACAGAATTCTCTTTGAAATTACAGCAGGGGCCTTGGGCAAAGCA
  CCCAGTAAAGTGACTGATGCCCTTCCAGAGCCAGAACCTCCAGGAGCGATGGCTGCCTCA
  GAGGATGAGGAGGAGGAGGAAGAGGCTCTGGAGGCCATGCAGTCCCGGCTGGCCACACTC
  CGCAGCTAG
  
  Clone variation with respect to NM_016079.3
  246 a=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016079 unedited
  ATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGTTCAGTTCGTCATGGGGCT
  GTTTGGAAAGACCCAGGAGAAGCCGCCCAAAGAACTGGTCAATGAGTGGTCATTGAAGAT
  AAGAAAGGAAATGAGAGTTGTTGACAGGCAAATAAGGGATATCCAAAGAGAAGAAGAAAA
  AGTGAAACGATCTGTGAAAGATGCTGCCAAGAAGGGCCAGAAGGATGTCTGCATAGTTCT
  GGCCAAGGAGATGATCAGGTCAAGGAAGGCTGTGAGCAAGCTGTATGCATCCAAAGCCCA
  CATGAACTCAGTGCTCATGGGGATGAAGAACCAGCTCGCGGTCTTGCGAGTGGCTGGTTC
  CCTGCAGAAGAGCACAGAAGTGATGAAGGCCATGCAAAGTCTTGTGAAGATTCCAGAGAT
  TCAGGCCACCATGAGGGAGTTGTCCAAAGAAATGATGAAGGCTGGGATCATAGAGGAGAT
  GTTAGAGGACACTTTTGAAAGCATGGACGATCAGGAAGAAATGGAGGAAGAAGCAGAAAT
  GGAAATTGACAGAATTCTCTTTGAAATTACAGCAGGGGCCTTGGGCAAAGCACCCAGTAA
  AGTGACTGATGCCCTTCCAGAGCCAGAACCTCCAGGAGCGATGGCTGCCTCANACGATGA
  GGAGGAGGAGGAAGAGGCTCTGGAGGCCATGCAGTCCCGGCTGGCCACACTCCGCAGCTA
  GGGGCTGCCTACCCCGCTGGGTGTGCACACACTCCTCTCAAGAGCTGCCATTNTATGTGT
  CTCTTGCACTACACCNTCTGTGTGAGGACTACCATTTTGGAGAAGGNTCTGTNTGNCTCT
  TTTCATTNCTCTGCCCAGTTNNTGGGATCGCANAAGGGATNGNTCTTATNAAAGTGGCAT
  AAATAAATGCATCATTTNNNTAGAGATAGACAGATTTATCTTATTNGTGGGGAGGGGAAA
  GAAATNCATCTGCTCATGAAGCCTTNCTGAAAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016079 unedited
  NNTTTTAGCTTTGGACCGGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTGN
  GAGAGAAGGATCTTGCTATGTTGCCCAGGCTGGTCTTGAACTCCTATTCTCAAGAGAGCC
  TCCTGCCTCAGCCTTGTAAAGCACTGGGATTATAGGCATGAACCACCGCACCCAGCCAAG
  ATTGCCATTTTGTATGATGAGACTGGAAGGACCCCATTGTTTCAGGATTTTGCTACAATA
  TACAAAAAACAATCTGTGAGACAGTGGCTGGGCTTTTTTCCTGCCTGATTAGTTCAGTGC
  ACATACAACTTGGACCAGAGGATCTGGGTTTGAATCCCATCTCTGATACTTCCCAAACTG
  AGCTGTTTTCCTTATTTGTAAAGACTAAGATCGCGTATGTCAAAGAGCTCTGTAAACTCT
  CAACACATACAAAGTACTACTGCTGAAATGATTTATAAATGTATCTTAAGAATGTCCCAG
  ATGAGTGAATGTTGGTTATGATGCATGACCACACCTTAGTGTGCTCCTGGTCAGCTCCTG
  ACTTTGAGAAAACACAGATAGGAAGGCCCATGTCCCACGCACAAGACAACCAGCTCGAAG
  CAGGGACAGGCAATGAGAGGTGAAAGAGACCTAGTACTACAGCTGTCTCATCTGGGTGCA
  ACTTGCAATGGGAAGGAAGGGACAGAAGCTCTAAATAGACCCACATGCACACCCCAGGAC
  ATAATGCAGCCACCAGCCAACAGGACAAGCAGCACATTTCCCCAGCATCCTCTTGTTTAA
  ATAGAAACAGAATGGCTGCCATACCATGTCTGTGAAAGCCGGGAGCAGCAACCACAGAGC
  AAAGTCACTTCTGCTGAGCTCCCAAATCCATATGCCTGGCCTAGCAACTCTTTCCACACC
  AA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016079
• Insert Size: 2080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016079.2, NP_057163.1
• RefSeq Size: 3174 bp
• RefSeq ORF: 669 bp
• Locus ID: 51652
• UniProt ID: Q9Y3E7
• Cytogenetics: 2p11.2
• Domains: DUF279
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes a protein that sorts transmembrane proteins into lysosomes/vacuoles via the multivesicular body (MVB) pathway. This protein, along with other soluble coiled-coil containing proteins, forms part of the ESCRT-III protein complex that binds to the endosomal membrane and recruits additional cofactors for protein sorting into the MVB. This protein may also co-immunoprecipitate with a member of the IFG-binding protein superfamily. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ring finger protein 103 (RNF103) gene. [provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as Vps24alpha).