Heparanase 1 (HPSE) (NM_006665) Human Untagged Clone

SKU SC111172

HPSE (untagged)-Human heparanase (HPSE), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Heparanase 1
• Synonyms: HPA; HPA1; HPR1; HPSE1; HSE1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111172 sequence for NM_006665 edited (data generated by NextGen Sequencing); ATGCTGCTGCGCTCGAAGCCTGCGCTGCCGCCGCCGCTGATGCTGCTGCTCCTGGGGCCG; CTGGGTCCCCTCTCCCCTGGCGCCCTGCCCCGACCTGCGCAAGCACAGGACGTCGTGGAC; CTGGACTTCTTCACCCAGGAGCCGCTGCACCTGGTGAGCCCCTCGTTCCTGTCCGTCACC; ATTGACGCCAACCTGGCCACGGACCCGCGGTTCCTCATCCTCCTGGGTTCTCCAAAGCTT; CGTACCTTGGCCAGAGGCTTGTCTCCTGCGTACCTGAGGTTTGGTGGCACCAAGACAGAC; TTCCTAATTTTCGATCCCAAGAAGGAATCAACCTTTGAAGAGAGAAGTTACTGGCAATCT; CAAGTCAACCAGGATATTTGCAAATATGGATCCATCCCTCCTGATGTGGAGGAGAAGTTA; CGGTTGGAATGGCCCTACCAGGAGCAATTGCTACTCCGAGAACACTACCAGAAAAAGTTC; AAGAACAGCACCTACTCAAGAAGCTCTGTAGATGTGCTATACACTTTTGCAAACTGCTCA; GGACTGGACTTGATCTTTGGCCTAAATGCGTTATTAAGAACAGCAGATTTGCAGTGGAAC; AGTTCTAATGCTCAGTTGCTCCTGGACTACTGCTCTTCCAAGGGGTATAACATTTCTTGG; GAACTAGGCAATGAACCTAACAGTTTCCTTAAGAAGGCTGATATTTTCATCAATGGGTCG; CAGTTAGGAGAAGATTTTATTCAATTGCATAAACTTCTAAGAAAGTCCACCTTCAAAAAT; GCAAAACTCTATGGTCCTGATGTTGGTCAGCCTCGAAGAAAGACGGCTAAGATGCTGAAG; AGCTTCCTGAAGGCTGGTGGAGAAGTGATTGATTCAGTTACATGGCATCACTACTATTTG; AATGGACGGACTGCTACCAGGGAAGATTTTCTAAACCCTGATGTATTGGACATTTTTATT; TCATCTGTGCAAAAAGTTTTCCAGGTGGTTGAGAGCACCAGGCCTGGCAAGAAGGTCTGG; TTAGGAGAAACAAGCTCTGCATATGGAGGCGGAGCGCCCTTGCTATCCGACACCTTTGCA; GCTGGCTTTATGTGGCTGGATAAATTGGGCCTGTCAGCCCGAATGGGAATAGAAGTGGTG; ATGAGGCAAGTATTCTTTGGAGCAGGAAACTACCATTTAGTGGATGAAAACTTCGATCCT; TTACCTGATTATTGGCTATCTCTTCTGTTCAAGAAATTGGTGGGCACCAAGGTGTTAATG; GCAAGCGTGCAAGGTTCAAAGAGAAGGAAGCTTCGAGTATACCTTCATTGCACAAACACT; GACAATCCAAGGTATAAAGAAGGAGATTTAACTCTGTATGCCATAAACCTCCATAATGTC; ACCAAGTACTTGCGGTTACCCTATCCTTTTTCTAACAAGCAAGTGGATAAATACCTTCTA; AGACCTTTGGGACCTCATGGATTACTTTCCAAATCTGTCCAACTCAATGGTCTAACTCTA; AAGATGGTGGATGATCAAACCTTGCCACCTTTAATGGAAAAACCTCTCCGGCCAGGAAGT; TCACTGGGCTTGCCAGCTTTCTCATATAGTTTTTTTGTGATAAGAAATGCCAAAGTTGCT; GCTTGCATCTGA; ; Clone variation with respect to NM_006665.5; 920 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006665 unedited; TTGATATTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACCAGCCAAAATGCTGCT; GCGCTCGAAGCCTGCGCTGCCGCCGCCGCTGATGCTGCTGCTCCTGGGGCCGCTGGGTCC; CCTCTCCCCTGGCGCCCTGCCCCGACCTGCGCAAGCACAGGACGTCGTGGACCTGGACTT; CTTCACCCAGGAGCCGCTGCACCTGGTGAGCCCCTCGTTCCTGTCCGTCACCATTGACGC; CAACCTGGCCACGGACCCGCGGTTCCTCATCCTCCTGGGTTCTCCAAAGCTTCGTACCTT; GGCCAGAGGCTTGTCTCCTGCGTACCTGAGGTTTGGTGGCACCAAGACAGACTTCCTAAT; TTTCGATCCCAAGAAGGAATCAACCTTTGAAGAGAGAAGTTACTGGCAATCTCAAGTCAA; CCAGGATATTTGCAAATATGGATCCATCCCTCCTGATGTGGAGGAGAAGTTACGGTTGGA; ATGGCCCTACCAGGAGCAATTGCTACTCCGAGAACACTACCAGAAAAAGTTCAAGAACAG; CACCTACTCAAGAAGCTCTGTAGATGTGCTATACACTTTTGCAAACTGCTCAGGACTGGA; CTTGATCTTTGGCCTAAATGCGTTATTAAGAACAGCAGATTTGCAGTGGAACAGTTCTAA; TGCTCAGTTGCTCCTGGACTACTGCTCTTCCAAGGGGTATAACATTTCTTGGGAACTAGG; CAATGAACCTAACAGTTTCCTTAAGAAAGCTGATATTTTCATCAATGGGTCGCAGTTAGG; AGAAAGATTTATTCAATTGCATAAACTTCTAAGAAAGTCCACCTTCAAAATGCAAACTCT; ATGTCCTGNATGTGGTCANCTCGAGAAAGACGCTAAGTGCTGAAGACTTCTGAAGCTGNT; GGAGAAGTGATGATCAGTACATNCATACTACTATTGAATNACGGCTGCTACAGGAAGAAT; CTAACCTA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006665 unedited; TCTATGAACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT; CAGGACTAGTATATTTTATTTTCAAATGCAAGCAGCAACTTTGGCATTTCTTATCACAAA; AAAACTATATGAAAAAAGCTGGCAAGCCCAGTGAACTTCCTGGCCGGAGAGGTTTTTCCA; TTAAAGGTGGCAAGGTTTGATCATCCACCATCTTTAAAGTTAAACCATTGAGTTGGACAG; ATTTGGAAAGTAATCCATGAGGTCCCAAAGGTCTTAAAAGGTATTTATCCACTTGCTTGT; TAAAAAAAGGATAGGGTAACCGCAAGTACTTGGTGACATTATGGAGGTTTATGGCATACA; GAGTTAAATCTCCTTCTTTATACCTTGGATTGTCAGTGTTTGTGCAATGAAGGTATACTC; GAAGCTTCCTTCTCTTTGAACCTTGCACGCTTGCCATTAACACCTTGGTGCCCACCAATT; TCTTGAACAGAAGAGATAGCCAATAATCAGGTAAAGGATCGAAGTTTTCATCCACTAAAT; GGTAGTTTCCTGCTCCAAAGAATACTTGCCTCATCACCACTTCTATTCCCATTCGGGCTG; ACAGGCCCAATTTATCCAGCCACATAAAGCCAGCTGCAAAGGTGTCGGATAGCAAGGGCG; CTCCGCCTCCATATGCAGAGCTTGTTTCTCCTAACCAGACCTTCTTGCCAGGCCCTGGTG; CTCTCAACCACCTGNAAAAACTTTTTGCACAGATGAAAATAAAATGTCCAATACATCAGG; GTTTAGAAAAATCTCCCTGGTAGCAGTCCGTCCATTCAAATAGTAGTGATGCCATGTAAC; TGAATCAATCACTTCTCCACCACCTTCAGGAAGCTCTTCAGCATCTTACCGTCTTTCTTC; GAGCTGACCAACTCAGACCATAGAGTTTGCATTTTTGAGGGGACTTCCTTAAAGTTATGC; AATGATAAATCTCTTCTACTGCGACCATGAC;
• Restriction Sites: Please inquire
• ACCN: NM_006665
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006665.2, NP_006656.1
• RefSeq Size: 3726 bp
• RefSeq ORF: 1632 bp
• Locus ID: 10855
• UniProt ID: Q9Y251
• Cytogenetics: 4q21.23
• Domains: Glyco_hydro_79n
• Protein Families: Secreted Protein
• Protein Pathways: Glycosaminoglycan degradation, Metabolic pathways
• Summary: Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]; Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.