UBL4A (NM_014235) Human Untagged Clone

SKU SC111067

UBL4A (untagged)-Human ubiquitin-like 4A (UBL4A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: UBL4A
• Synonyms: DX254E; DXS254E; G6PD; GDX; GET5; MDY2; TMA24; UBL4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111067 sequence for NM_014235 edited (data generated by NextGen Sequencing); ATGCAGCTGACGGTGAAGGCGCTGCAGGGCCGCGAGTGCAGCCTGCAGGTGCCAGAGGAC; GAGCTGGTGTCCACGCTGAAGCAGCTGGTCTCCGAGAAGCTGAACGTCCCAGTGCGCCAG; CAGCGGCTGCTGTTCAAGGGCAAGGCCCTGGCAGATGGGAAACGACTCTCGGATTATAGC; ATCGGGCCCAACTCCAAGCTCAACCTAGTGGTCAAACCCCTGGAGAAGGTGCTACTAGAA; GAAGGCGAGGCCCAGAGGCTGGCCGACTCCCCACCCCCGCAGGTCTGGCAGCTGATCTCC; AAAGTCTTGGCCCGCCACTTCAGTGCGGCAGATGCCAGCAGGGTCCTGGAACAGCTACAG; AGGGATTACGAGAGGTCCCTGAGTCGCCTGACGCTGGACGACATCGAACGGTTGGCCAGC; CGCTTCCTGCACCCTGAAGTGACTGAGACAATGGAGAAGGGCTTCTCCAAATAG; ; Clone variation with respect to NM_014235.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014235 unedited; GCATTTGTATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGGACGCCGTCGCG; ACCGCCATGCAGCTGACGGTGAAGGCGCTGCAGGGCCGCGAGTGCAGCCTGCAGGTGCCA; GAGGACGAGCTGGTGTCCACGCTGAAGCAGCTGGTCTCCGAGAAGCTGAACGTCCCAGTG; CGCCAGCAGCGGCTGCTGTTCAAGGGCAAGGCCCTGGCAGATGGGAAACGACTCTCGGAT; TATAGCATCGGGCCCAACTCCAAGCTCAACCTAGTGGTCAAACCCCTGGAGAAGGTGCTA; CTAGAAGAAGGCGAGGCCCAGAGGCTGGCCGACTCCCCACCCCCGCAGGTCTGGCAGCTG; ATCTCCAAAGTCTTGGCCCGCCACTTCAGTGCGGCAGATGCCAGCAGGGTCCTGGAACAG; CTACAGAGGGATTACGAGAGGTCCCTGAGTCGCCTGACGCTGGACGACATCGAACGGTTG; GCCAGCCGCTTCCTGCACCCTGAAGTGACTGAGACAATGGAGAAGGGCCTTCTCCAAATA; GAATTCTCGGAGCATGGGGAGGTGCCCAACGCCAGGCTACCGCTGCATGTCGCACTAAGT; GTGTTCTCCCTGTGCAGTTGGGCTCATCATCATCATAGCTGGCATGTACCTGGCTCTGGC; CAGGTGCTAGGCACTCCCTCACAGCTTGACCTGCGTTTGCTTCCACACCCTCAGAAGAGG; AGAGCCGGCACAGCAGAAGCACCGGCGATGAAGTGGCACAGCCCCCATCCGGAATACAGC; GGCTTTCTGTAAGTGCCTTGCTGTGACAAAGAGGATGGGCCCGCTTGGAAAGCTGGCCTC; TTCGATGTCATGTTTAACCCCCCGAGAATAACCCTCAGTGACTCCTTCCGCTACTCGAAG; GCAAGCACCTGTCCCTGACTCTGGCGGGTCCACATAGCCTTAGGTTACTTGCAGAAGGCA; GTGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014235 unedited; TTTTACTCTGNACCCGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTCTGACCACGAAAAAATGTTTATTTGCTGGGGGCATCTGAAACAGCAAAG; GGGGTACACAAAAAAAATGCTGCTCTGGCAGTGAGGGAGGGATTCCGTGGAAGCTGCAGG; CAGCCATCTTTTCATCCTCGGAGGGGGGGGCCTGGGATGTACCAAATACCAACGCAAAGG; GAGGCAAAAAACACTGGGTGAAATTGCTCACCTGCTGCCAATGCCAATACCACTGGACTT; TTCACTTTCATAAGCCAAAAAATTTCCGTTAACTGATTAAACAAATTGCCTTTATTGTTA; AACCAATGATTGGATATCAAAAACAGCCTAGCTAATATAGCTCCTTTTGGATAAAAGTAA; AAGCCTCACATTCACCTTTAATGTTATTTCACTTTTTCCAAAATCAATTTCCTGGCACCA; CACAAACAAAAACACTGGTAAATGTATCACCTGAACTGCAGGCCACACATGCTCACGAGT; GTGTCCCTGCCGTAGGTGGCTTTGAAGCCATATACATGGCTCTCCAGCTCTCGTGGGGGG; CCAGGGGCTTCTGTGTGCTCTTCAAAGCCTTCACCCATTGGGCCTCTGTCCATCTTTCCA; GCCTGGGGACCCAGTATTTGCCCCACAAGGCCCAGGCAGGAGGGCCTNCAGCACGCGCAG; CCCTGCCACACTGTGAGCAGCTCACTTTGCAGGNNTGAAGGGCCCGNANTGGCGTCCTCC; TGATGTGGCTTCCTTTCAGCCTCTACACCCCTGGGGGCCCTCCTCAGGGAGCAGGTGGTT; AGATGTCCTTCTGTGGCACACACACGNAGGNTTGAAGGAAGATGAAACCGGACCCATNCA; TGCTTTTGCCCCACCCTGCACCCNAGGTNTNTTCTAGGCACAAGGACCCCCAAGGGGACC; CTGAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_014235
• Insert Size: 2350 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014235.2, NP_055050.1
• RefSeq Size: 2322 bp
• RefSeq ORF: 474 bp
• Locus ID: 8266
• UniProt ID: P11441
• Cytogenetics: Xq28
• Domains: UBQ
• Protein Families: Druggable Genome
• Summary: As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation (PubMed:20676083, PubMed:21636303, PubMed:21743475, PubMed:28104892). The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum (PubMed:20676083, PubMed:28104892, PubMed:25535373). Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome (PubMed:28104892). Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum (PubMed:21743475). The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome (PubMed:21636303).[UniProtKB/Swiss-Prot Function]