TRAF2 (NM_021138) Human Untagged Clone

CAT#: SC111044

TRAF2 (untagged)-Human TNF receptor-associated factor 2 (TRAF2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRAF2
• Synonyms: MGC:45012; RNF117; TRAP; TRAP3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021138 edited
  GAATTCGGCACGAGGGAACCCGTGGTAGCTAAAGGGAGTGCACTAGCCAGCCTCAGGTGT
  GCTACGTTCCCGCTCTCGGGGCTGGAGGGGAACCCGTGGTAGCTATAGGGAGTGCACTAG
  CCAGCCTCAGATGTGCTACGTTCCCGCTCTCAGGGCTGGAGGGGAACCCGTGGTAGCTAA
  AGGGAGTGCACTAGCCAGCTCCAGGTGTGCTACATTCCCGCTCTCGGGGCTGGAGGGGAA
  CCCGTGGTAGCTAAAGGGAGTGCACTAGCCAGCCCCAGGTGTGCTACATTCCCGCTCTAG
  GGGCTGGTCTGGCGGCATGGCTCTCTCCTGAGTGCCTTCAGAGCCTGAGTGCCAGCAGGC
  AGGCGTCTCCTGCCCAGTGGGTTTGGGCTTTGTTCGCGGGGGTCACAGCTCTCATGGCTG
  CAGCTAGCGTGACCCCCCCTGGCTCCCTGGAGTTGCTACAGCCCGGCTTCTCCAAGACCC
  TCCTGGGGACCAAGCTGGAAGCCAAGTACCTGTGCTCCGCCTGCAGAAACGTCCTCCGCA
  GGCCCTTCCAGGCGCAGTGTGGCCACCGGTACTGCTCCTTCTGCCTGGCCAGCATCCTCA
  GCTCTGGGCCTCAGAACTGTGCTGCCTGTGTTCACGAGGGCATATATGAAGAAGGCATTT
  CTATTTTAGAAAGCAGTTCGGCCTTCCCAGATAATGCTGCCCGCAGGGAGGTGGAGAGCC
  TGCCGGCCGTCTGTCCCAGTGATGGATGCACCTGGAAGGGGACCCTGAAAGAATACGAGA
  GCTGCCACGAAGGCCGCTGCCCGCTCATGCTGACCGAATGTCCCGCGTGCAAAGGCCTGG
  TCCGCCTTGGTGAAAAGGAGCGCCACCTGGAGCACGAGTGCCCGGAGAGAAGCCTGAGCT
  GCCGGCATTGCCGGGCACCCTGCTGCGGAGCAGACGTGAAGGCGCACCACGAGGTCTGCC
  CCAAGTTCCCCTTAACTTGTGACGGCTGCGGCAAGAAGAAGATCCCCCGGGAGAAGTTTC
  AGGACCACGTCAAGACTTGTGGCAAGTGTCGAGTCCCTTGCAGATTCCACGCCATCGGCT
  GCCTCGAGACGGTAGAGGGTGAGAAACAGCAGGAGCACGAGGTGCAGTGGCTGCGGGAGC
  ACCTGGCCATGCTACTGAGCTCGGTGCTGGAGGCAAAGCCCCTCTTGGGAGACCAGAGCC
  ACGCGGGGTCAGAGCTCCTGCAGAGGTGCGAGAGCCTGGAGAAGAAGACGGCCACTTTTG
  AGAACATTGTCTGCGTCCTGAACCGGGAGGTGGAGAGGGTGGCCATGACTGCCGAGGCCT
  GCAGCCGGCAGCACCGGCTGGACCAAGACAAGATTGAAGCCCTGAGTAGCAAGGTGCAGC
  AGCTGGAGAGGAGCATTGGCCTCAAGGACCTGGCGATGGCTGACTTGGAGCAGAAGGTCT
  TGGAGATGGAGGCATCCACCTACGATGGGGTCTTCATCTGGAAGATCTCAGACTTCGCCA
  GGAAGCGCCAGGAAGCTGTGGCTGGCCGCATACCCGCCATCTTCTCCCCAGCCTTCTACA
  CCAGCAGGTACGGCTACAAGATGTGTCTGCGTATCTACCTGAACGGCGACGGCACCGGGC
  GAGGAACACACCTGTCCCTCTTCTTTGTGGTGATGAAGGGCCCGAATGACGCCCTGCTGC
  GGTGGCCCTTCAACCAGAAGGTGACCTTAATGCTGCTCGACCAGAATAACCGGGAGCACG
  TGATTGACGCCTTCAGGCCCGACGTGACTTCATCCTCTTTTCAGAGGCCAGTCAACGACA
  TGAACATCGCAAGCGGCTGCCCCCTCTTCTGCCCCGTCTCCAAGATGGAGGCAAAGAATT
  CCTACGTGCGGGACGATGCCATCTTCATCAAGGCCATTGTGGACCTGACAGGGCTCTAAC
  TGCCCCCTACTGGTGTCTGGGGGTTGGGGGCAGCCAGGCACAGCCGGCTCACGGAGGGGC
  CACCACACTGGGCCAGGGTCTCACTGTACAAGTGGGCAGGGGCCGCGCTTGGGCGCTTGG
  GAGGGTGTCGGCCTGCAGCCAAGTTCACTGTCACGGGGGAAGGAGCCACCAGCCAGTCCT
  CAGATTTCAGAGACTGCGGAGGGGCTTGGCAGACGGTCTTAGCCAAGGGCTGTGGTGGCA
  TTGGCCGAGGGTCTTCGGGTGCTTCCCAGCACAAGCTGCCCTTGCTGTCCTGTGCAGTGA
  AGGGAGAGGCCCTGGGTGGGGGACACTCAGAGTGGGAGCACATCCCAGCAGTGCCCATGT
  AGCAGGAGCACAGTGGATGGCCTTGTGTCCCTCGGGCATGACAGGCAGAAACGAGGGCTG
  CTCCAGGAGAAGGGCCTCCTGCTGGCCAGAGCAAGGAAGGCTGAGCAGCTTGGTTCTCCC
  CTCTGGCCCCTGGAGAGAAGGGGGAGCATTCCTAGACCCCTGGGTGCTTGTCTGCACAGA
  GCTCTGGTCTGTGCCACCTTGGCCAGGCTGGCTGTGGGAGGGTCTGGTCCCACGCCGCCT
  CTGCTCAGACCACTGTGTGGGAGGTGCACAGCACAGCCTGCGGGTAAAGTGTGAGAGCTT
  GCCATCCAGCTCACGAAGACAGAGTTATTAAACCATTCAAATCTCAAAAAAAAAAAAAAA
  AAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021138 unedited
  TACATAATTTGTAATACGACTCACTATAGGGCGGCACGCGAATTCGCACGAGAGGAACCC
  GTGGTAGCTAAAGGGAGTGCACTAGCCAGCCTCAGGTGTGCTACGTTCCCGCTCTCGGGG
  CTGGAGGGGAACCCGTGGTAGCTATAGGGAGTGCACTAGCCAGCCTCAGATGTGCTACGT
  TCCCGCTCTCAGGGCTGGAGGGGAACCCGTGGTAGCTAAAGGGAGTGCACTAGCCAGCTC
  CAGGTGTGCTACATTCCCGCTCTCGGGGCTGGAGGGGAACCCGTGGTAGCTAAAGGGAGT
  GCACTAGCCAGCCCCAGGTGTGCTACATTCCCGCTCTAGGGGCTGGTCTGGCGGCATGGC
  TCTCTCCTGAGTGCCTTCAGAGCCTGAGTGCCAGCAGGCAGGCGTCTCCTGCCCAGTGGG
  TTTGGGCTTTGTTCGCGGGGGTCACAGCTCTCATGGCTGCAGCTAGCGTGACCCCCCCTG
  GCTCCCTGGAGTTGCTACAGCCCGGCTTCTCCAAGACCCTCCTGGGGACCAAGCTGGAAG
  CCAAGTACCTGTGCTCCGCCTGCAGAAACGTCCTCCGCAGGCCCTTCCAGGCGCAGTGTG
  GCCACCGGTACTGCTCCTTCTGCCTGGCCAGCATCCTCAGCTCTGGGCCTCAGAACTGTG
  CTGCCTGTGTTCACGATGGCATATATGAAGAAGGCATTTCTATTTTAGAAAGCAGTTCGG
  CCTTCCCAGATAATGCTGCCCGCANGGAGGTGGAGAGCCTGCNCGCCGTCTGTCCCAGTG
  ATGGATGCACCTGGAAGGNGACCCCTGAAGAATACGAGAGCTGCCACGAAAGCCGCTGNC
  CGCTCATGCTGACCGAAATGTCCCGCGTGNCAAGGCCTTGGTCGCCTGGNTGAAAATGAG
  CGCACCTGGAGCACGGTGCCCCGANAGATCCTGAGCTGCCNGCATTGCGGNCACCTGCTG
  CGGACANAGTGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021138 unedited
  TTTTTTTTTTTTTTTTTNGAAATTTGATGGTTTAATAACTCTGTCTTCGTGAGCTGGATG
  GCAAGCTCTCACACTTTACCCGCAGGCTGTGCTGTGCACCTCCCACACAGTGGTCTGAGC
  AAAGGCGGCGTGGGACCAGACCCTCCCACAGCCAGCCTGGCCAAGGTGGCACAGACCAGA
  GCTCTGTGCAGACAAGCACCCAGGGGTCTAGGAATGCTCCCCCTTCTCTCCAGGGGCCAG
  AGGGGAGAACCAAGCTGCTCAGCCTTCCTTGCTCTGGCCAGCAGGAGGCCCTTCTCCTGG
  AGCAGCCCTCGTTTCTGCCTGTCATGCCCGAGGGACACAAGGCCATCCACTGTGCTCCTG
  CTACATGGGCACTGCTGGGATGTGCTCCCACTCTGAGTGTCCCCCACCCAGGGCCTCTCC
  CTTCACTGCACAGGACAGCAAGGGCAGCTTGTGCTGGGAAGCACCCGAAGACCCTCGGCC
  AATGCCACCACAGCCCTTGGCTAAGACCGTCTGCCAAGCCCCTCCGCAGTCTCTGAAATC
  TGAGGACTGGCTGGTGGCTCCTTCCCCCGTGACAGTGAACTTGGCTGCAGGCCGACACCC
  TCCCAAGCGCCCAAGCGCGGCCCCTGCCCACTTGTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021138
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021138.3, NP_066961.2
• RefSeq Size: 2298 bp
• RefSeq ORF: 1506 bp
• Locus ID: 7186
• UniProt ID: Q12933
• Cytogenetics: 9q34.3
• Domains: zf-TRAF, RING, MATH
• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Apoptosis, MAPK signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer
• Gene Summary: The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes a longer isoform (1).