Radixin (RDX) (NM_002906) Human Untagged Clone

SKU SC110995

RDX (untagged)-Human radixin (RDX)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Radixin
• Synonyms: DFNB24
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110995 sequence for NM_002906 edited (data generated by NextGen Sequencing); ATGCCGAAACCAATCAACGTAAGAGTAACTACAATGGATGCTGAGCTGGAATTTGCCATT; CAGCCCAATACAACTGGCAAACAACTTTTTGACCAGGTGGTGAAAACAGTTGGTTTGCGT; GAGGTCTGGTTTTTTGGGCTGCAGTATGTAGACAGCAAAGGTTATTCTACATGGCTTAAA; CTAAATAAAAAGGTAACACAGCAGGATGTTAAAAAAGAGAATCCTTTACAGTTCAAGTTT; AGAGCTAAATTCTTTCCTGAAGATGTTTCTGAGGAATTAATTCAAGAAATAACCCAGAGA; CTCTTCTTCTTGCAAGTTAAAGAAGCCATCTTAAATGATGAGATATATTGCCCGCCAGAA; ACTGCAGTTCTTTTGGCTTCCTATGCTGTCCAAGCCAAGTATGGAGATTACAATAAAGAG; ATTCATAAGCCAGGCTACCTGGCTAATGATAGACTCCTACCCCAGCGTGTATTGGAACAA; CACAAACTAACAAAAGAACAGTGGGAAGAAAGAATACAGAACTGGCATGAAGAACATAGA; GGAATGTTAAGGGAGGATTCTATGATGGAATACCTGAAGATTGCACAAGATCTAGAAATG; TATGGAGTCAACTATTTTGAAATAAAAAATAAAAAAGGAACTGAATTGTGGCTAGGTGTT; GATGCTTTGGGTCTGAATATTTATGAGCATGACGACAAGTTAACACCTAAAATTGGTTTT; CCCTGGAGTGAAATCAGAAATATTTCATTTAATGACAAAAAATTTGTTATAAAGCCAATC; GACAAAAAGGCACCTGATTTTGTGTTTTATGCACCTCGTCTGAGAATCAATAAGCGGATT; TTGGCCTTATGTATGGGAAACCATGAACTATACATGCGAAGAAGGAAGCCTGATACTATT; GAAGTACAACAGATGAAGGCTCAGGCTAGGGAGGAGAAACATCAGAAGCAGTTGGAAAGG; GCACAATTAGAGAATGAAAAGAAGAAAAGAGAAATAGCAGAAAAGGAAAAGGAAAGAATA; GAACGTGAAAAGGAAGAGCTAATGGAACGTCTAAAACAAATTGAAGAGCAGACAATTAAA; GCTCAGAAAGAACTAGAAGAACAGACTCGAAAAGCTCTAGAACTGGATCAAGAACGAAAA; CGAGCAAAAGAAGAAGCAGAACGACTTGAAAAGGAGCGTCGAGCTGCTGAAGAGGCAAAG; TCTGCCATAGCAAAACAAGCTGCCGACCAGATGAAGAATCAGGAGCAGCTAGCAGCAGAA; CTTGCTGAATTCACTGCCAAGATTGCACTTCTAGAGGAAGCCAAGAAGAAAAAGGAAGAG; GAAGCTACTGAGTGGCAACACAAAGCTTTTGCAGCCCAGGAAGACTTGGAAAAGACCAAA; GAAGAGTTAAAAACTGTGATGTCTGCCCCCCCTCCACCTCCACCACCACCAGTCATTCCT; CCAACAGAAAACGAACATGATGAACACGATGAGAATAATGCTGAAGCTAGTGCTGAATTA; TCAAATGAAGGGGTAATGAACCATAGAAGCGAGGAAGAACGTGTAACCGAAACACAGAAA; AATGAGCGTGTTAAGAAGCAACTTCAGGCATTAAGTTCAGAATTAGCCCAAGCCAGAGAT; GAAACCAAGAAAACACAAAATGATGTTCTTCATGCTGAGAATGTTAAAGCAGGCCGTGAT; AAGTACAAGACTCTGCGACAGATTCGACAAGGCAATACAAAGCAGCGTATCGATGAGTTT; GAAGCAATGTGA; ; Clone variation with respect to NM_002906.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002906 unedited; TGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGCGGAGGCGCC; GAGCGGCCATATTGCGGAGCTGTCTGCGGTGGCGGCGGCGCCTCTCGTCTCCCGCGGCCC; AGCGCTCGCACCACCGCTTCTCCCTCCCTGTCGCAGCCGCGCCGCCGCGCAGCGCCCCAG; CCACACGCCGGCGGGCAGAAGCCGCCCGCTCTCCGGAAAGTGATAACAGAATTCATTGAA; GTGGAGAATTTTTAAAGAAGGTAACAAAAAGAGAAAGAAAATGCCGAAACCAATCAACGT; AAGAGTAACTACAATGGATGCTGAGCTGGAATTTGCCATTCAGCCCAATACAACTGGCAA; ACAACTTTTTGACCAGGTGGTGAAAACAGTTGGTTTGCGTGAGGTCTGGTTTTTTGGGCT; GCAGTATGTAGACAGCAAAGGTTATTCTACATGGCTTAAACTAAATAAAAAGGTAACACA; GCAGGATGTTAAAAAAGAGAATCCTTTACAGTTCAAGTTTAGAGCTAAATTCTTTCCTGA; AGATGTTTCTGAGGAATTAATTCAAGAAATAACCCAGAGACTCTTCTTCTTGCAAGTTAA; AGAAGCCATCTTAAATGATGAGATATATTGCCCGCCAGAAACTGCAGTTCTTTTGGCTTC; CTATGCTGTCCAAGCCAAGTATGGAGATTACCATAAAGAGATTCATAAGCCAGGCTACCT; GGCTAATGATAGACTCCTACCCCAGCGTGTATTGGAACACACAAACTAACANAAGACAGT; GGGAAGAAGATACAGACTGGCATGAAGACATAGAGGATGTTANGGGGAGATCATGATGGN; ATACCTGAGATGCCCAGATCTAAAAGTATGGAGCAACTATTTGGAATAAAAATAAAAGGA; ACTGATGTGCTAGTNGTGAGCTTGGGTCTGATATTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002906 unedited; TGTACGCGGCCGCATCTATAGTCGGTTTTTTTTTTTTTTTTTTTTGCATGAGCTTTTGTC; ACTTTATTGTTAACCAATGAATATTATCCAAAATTAGAGATGTAATTGTAACTTAATTGT; ACAACACAAAATTATGCTAATGGTAAAAGCCTACTGGGATTTACCAAATACTCATTAGTG; TATTTTTACATTGACTATATGAACATGTGCTCGCGACTGCTAATAAGTTATAATTGGTTT; AATCTCTAAAAAATCCATATTGCAAATGGTTCTTGTTTAAAAAAATTCACACAGCCTTAA; AAATGGATTAAATCCATTTTAATCCTAATCTACAAATAGATCATAAACAGCAAAATATAA; CTGATAATTTTTCAATACTGTATCAAACTGATGTAGCTATGGTAGTGCACATAATTCAAA; ATGAGGAAAATTAACAAAAATCCAATGAAAGTTCAGGTTTTTATCTATTATGCAATTGCT; TTAAAGTGAACATTTATGTCTCAAAACATGAATAGTACGGTCCACCATCCATTCCTTTGA; TGTACTGCAAGCAGGAATATTCTCTTCATTGGATTCTGAATCTTGTGTACAACCCTGGCA; CATCATTACTTCAAGGGTTATCTCCTTGTTTTTGCTCCTCCAGCTCCCTGTTTTCTTTTT; TTGTTTTTTGTTTTTTTTTCGTGTTTGGTTCTGTTGTTTTTTTTTTTTTTTTCCTTTTTT; TTTTGGTGTTTGTTGTTTCTTATTTTTTTTTTGCTTTTTTGTAGTGTTTGTGTGAGGGTA; TNTTTCGTGTTTTGTTCTGGGCTTCCATTTTCGGCGTGGTCTTGCTCAGGTTGNTCTGCT; GTTACCCCGTGGTGCATGTGTGTGTGTTTCCCATGTGATACTTCACGCCAATGGGCTGTA; TGCCCGTGCTGGTTATTGCTAACACTTGTCGAAGCTTATTCTTCACCATATTACTCTCCT; CTTATTTTTCTATATCCTCCTCTCCTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_002906
• Insert Size: 4730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002906.3, NP_002897.1
• RefSeq Size: 4498 bp
• RefSeq ORF: 1752 bp
• Locus ID: 5962
• UniProt ID: P35241
• Cytogenetics: 11q22.3
• Domains: B41, ERM
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton
• Summary: Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]; Transcript Variant: This variant (3) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a shorter C-terminus, compared to isoform 1.