Prion protein PrP (PRNP) (NM_000311) Human Untagged Clone

SKU SC110984

PRNP (untagged)-Human prion protein (PRNP), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Prion protein PrP
• Synonyms: AltPrP; ASCR; CD230; CJD; GSS; KURU; p27-30; PRIP; PrP; PrP27-30; PrP33-35C; PrPc
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110984 sequence for NM_000311 edited (data generated by NextGen Sequencing); ATGGCGAACCTTGGCTGCTGGATGCTGGTTCTCTTTGTGGCCACATGGAGTGACCTGGGC; CTCTGCAAGAAGCGCCCGAAGCCTGGAGGATGGAACACTGGGGGCAGCCGATACCCGGGG; CAGGGCAGCCCTGGAGGCAACCGCTACCCACCTCAGGGCGGTGGTGGCTGGGGGCAGCCT; CATGGTGGTGGCTGGGGGCAGCCTCATGGTGGTGGCTGGGGGCAGCCCCATGGTGGTGGC; TGGGGACAGCCTCATGGTGGTGGCTGGGGTCAAGGAGGTGGCACCCACAGTCAGTGGAAC; AAGCCGAGTAAGCCAAAAACCAACATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCA; GTGGTGGGGGGCCTTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACAT; TTCGGCAGTGACTATGAGGACCGTTACTATCGTGAAAACATGCACCGTTACCCCAACCAA; GTGTACTACAGGCCCATGGATGAGTACAGCAACCAGAACAACTTTGTGCACGACTGCGTC; AATATCACAATCAAGCAGCACACGGTCACCACAACCACCAAGGGGGAGAACTTCACCGAG; ACCGACGTTAAGATGATGGAGCGCGTGGTTGAGCAGATGTGTATCACCCAGTACGAGAGG; GAATCTCAGGCCTATTACCAGAGAGGATCGAGCATGGTCCTCTTCTCCTCTCCACCTGTG; ATCCTCCTGATCTCTTTCCTCATCTTCCTGATAGTGGGATGA; ; Clone variation with respect to NM_000311.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000311 unedited; TTTGTATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCGACCGAGAGCAGTCA; TTATGGCGAACCTTGGCTGCTGGATGCTGGTTCTCTTTGTGGCCACATGGAGTGACCTGG; GCCTCTGCAAGAAGCGCCCGAAGCCTGGAGGATGGAACACTGGGGGCAGCCGATACCCGG; GGCAGGGCAGCCCTGGAGGCAACCGCTACCCACCTCAGGGCGGTGGTGGCTGGGGGCAGC; CTCATGGTGGTGGCTGGGGGCAGCCTCATGGTGGTGGCTGGGGGCAGCCCCATGGTGGTG; GCTGGGGACAGCCTCATGGTGGTGGCTGGGGTCAAGGAGGTGGCACCCACAGTCAGTGGA; ACAAGCCGAGTAAGCCAAAAACCAACATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGG; CAGTGGTGGGGGGCCTTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATAC; ATTTCGGCAGTGACTATGAGGACCGTTACTATCGTGAAAACATGCACCGTTACCCCAACC; AAGTGTACTACAGGCCCATGGATGAGTACAGCAACCAGAACAACTTTGTGCACGACTGCG; TCAATATCACAATCAAGCAGCACACGGTCACCACAACCACCAAGGGGGAGAACTTCACCG; AGACCGACGTTAAGATGATGGAGCGCGTGGTTGAGCANATGTGTATCACCCAGTACGAGA; GGGAATCTCAGGCCTATTACCAGAGGAGATCGAGCATGGTCCTCTTCTCCTCTCCACCTG; GATCCTNCTGATCTCTTTCCTCATCTTCCTGATAGGGGGATGAGGAANGGTCTTNNCTGT; TTNCACCATCTTNCTAAATCTTNTTCAGCTTGAGGGAGGCGGNATCCACCTGCACCCTTT; TANGGGNGGGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000311 unedited; NGGCTTCACTCTGAACCCGCGGCCGCATNCTAAGATCGAGTTTTTTTTTTTTTTTTTTGG; TGCTCATCTTCGCTCGTTTAATTTCAGTCAGATATTAAACATTTACAATTTTTTTATATA; ACAAAACAAGAACATGCAAAGTTACAAATATAGAAAATATAAAGTACATGCATATTTCAA; AGACCTGTTAATGGTGTCCACTTTGGATTCTTACATGAAACGATTCAGTGCACATTGTAA; GCCTAAGGACCACGCAAAAGGGTTTCCCACATATTAAGTATTCAGTACCTTACAAAAGTT; AATGCATTAGACACTTCAGATGTTAACTGCTCTAAACAAAACTCCTAAGTCTGTCCTATG; CAATATATATTTTATATACATATATATTTTTACATAGAATACTCACAAAGTGCAAGCCAA; TAATAACATTGCAGAAAAGTAATACATATCTGCTAGGTGACAATATCAAACAATTCAGGG; AATAATTTTACTTTAATTAACATTAACAGAATTTCTTTTTCCACTTCAAATCAATCATAT; TTCTGTCATCTCCAACCTAAGATATTTTTTAGATTGTCTCCCTATTCTTTGATTCAAAAG; CCAATTACAGAAACTATGAACTTGACCTAATTCTGGTTTTTGACAATTATGAGACAGAAA; TAAAGAAATGCAAGCAGTTCTTTTCTTTGCACACTGACCATTTTTTAATTACATCATCCT; CTATGATGATGGTGCTTTCACAACTGCAGCTCTCCTGTATGTCAAAATCATTCTGGTTTC; CAGGTAAATGGACAAAGGAGATTTGCCTTCAGTGTCTAGAAAGCAATTACTTTTCAGCTG; CCTTAATTACTATAGTTAAAGAAAGGATGCCCATATAGGGCCTTTAAACANTAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_000311
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000311.2, NP_000302.1
• RefSeq Size: 2483 bp
• RefSeq ORF: 762 bp
• Locus ID: 5621
• UniProt ID: P04156
• Cytogenetics: 20p13
• Domains: prion
• Protein Families: ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Prion diseases
• Summary: The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]; Transcript Variant: This variant (1) represents the longest transcript. Variants 1-5 can all encode the same isoform (Prp).