PAR4 (PAWR) (NM_002583) Human Untagged Clone

SKU SC110969

PAWR (untagged)-Human PRKC, apoptosis, WT1, regulator (PAWR)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PAR4
• Synonyms: Par-4; PAR4
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110969 sequence for NM_002583 edited (data generated by NextGen Sequencing); ATGGCGACCGGTGGCTACCGGACCAGCAGCGGCCTCGGCGGCAGCACCACAGACTTCCTG; GAGGAGTGGAAGGCGAAACGCGAGAAGATGCGCGCCAAGCAGAACCCCCCGGGCCCGGCC; CCCCCGGGAGGGGGCAGCAGCGACGCCGCTGGGAAGCCCCCCGCGGGGGCTCTGGGCACC; CCGGCGGCCGCCGCTGCCAACGAGCTCAACAACAACCTCCCGGGCGGCGCGCCGGCCGCA; CCTGCCGTCCCCGGTCCCGGGGGCGTGAACTGCGCGGTCGGCTCCGCCATGCTGACGCGG; GCGGCCCCCGGCCCGCGGCGGTCGGAGGACGAGCCCCCAGCCGCCTCTGCCTCGGCTGCA; CCGCCGCCCCAGCGTGACGAGGAGGAGCCGGACGGCGTCCCAGAGAAGGGCAAGAGCTCG; GGCCCCAGTGCCAGGAAAGGCAAGGGGCAGATCGAGAAGAGGAAGCTGCGGGAGAAGCGG; CGCTCCACCGGCGTGGTCAACATCCCTGCCGCAGAGTGCTTAGATGAGTACGAAGATGAT; GAAGCAGGGCAGAAAGAGCGGAAACGAGAAGATGCAATTACACAACAGAACACTATACAG; AATGAAGCTGTAAACTTACTAGATCCAGGCAGTTCCTATCTGCTACAGGAGCCACCTAGA; ACAGTTTCAGGCAGATATAAAAGCACAACCAGTGTCTCTGAAGAAGATGTCTCAAGTAGA; TATTCTCGAACAGATAGAAGTGGGTTCCCTAGATATAACAGGGATGCAAATGTTTCAGGT; ACTCTGGTTTCAAGTAGCACACTGGAAAAGAAAATTGAAGATCTTGAAAAGGAAGTAGTA; AGAGAAAGACAAGAAAACCTAAGACTTGTGAGACTGATGCAAGATAAAGAGGAAATGATT; GGAAAACTCAAAGAAGAAATTGATTTATTAAATAGAGACCTAGATGACATAGAAGATGAA; AATGAACAGCTAAAGCAGGAAAATAAAACTCTTTTGAAAGTTGTGGGTCAGCTGACCAGG; TAG; ; Clone variation with respect to NM_002583.2; 597 t=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002583 unedited; TCCCGCCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGA; GCTTATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAAT; TCGGCACGAGGGAGAAACTTTGCAGGGTGGGGACCCGGCGGCTGCTGGCCGGTAGTGACT; GGTGGGCGCGCTCGAGGACTCCAAGGGGCGCAGCCCGGGGGCAGACCCTTGGGTCGGGCG; GGGATCTTACGCTTCCCTTACCCGCCCCCTTTTGTCTTTCACCTCAGCCCCGCCGGCTGC; TGTGGGAGCGGCGGCCGTCCCTCTCCTGGAGGTCGTCTCCTGGCATCCTCGGGGCCGCAG; GAAGGAAGAGGAGGCAGCGGCCGGAGCCCTGGTGGGCGGCCTGAGGTGAGAGCCCGACCG; GCCCCTTTGGGAATATGGCGACCGGTGGCTACCGGACCAGCAGCGGCCTCGGCGGCAGCA; CCACAGACTTCCTGGAGGAGTGGAAGGCGAAACGCGAGAAGATGCGCGCCAAGCAGAACC; CCCCGGGCCCGGCCCCCCCGGGAGGGGGCAGCAGCGACGCCGCTGGGAAGCCCCCCGCGG; NGGCTCTGGGCACCCCGGCGGCCGNCGCTGCCAACGAGCTCAACACAACCTCCCGGGCGG; CGCGCCGGCCGCACCTGCCGTCCCCGTCCCGGGGGCGTGACTGCGNNCGTCGCTCCGCCA; TGCTGACGCGGGCGGGCCCCGGCCGCNNGCGTTCGAGACGAACCCCCACCGNCTCTGCCT; CGGNTGCACCGCCGCCCANCGTGACAAAGGAGAACCCGGACGCGTCCCANAAAGGGCAGA; GCTCGGCCCCAGTGCCAGAANGGCAAGGGCANATCGAGAAAAGAACCTGCCGGAGACCGC; GCTCACCGGCGGGTCACATCCTGCCGCAAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002583 unedited; NNCCGGTATATTATGGACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTT; TTTCTAAATAATAATATTTAATAGAAAAAAAATCTGTATAATTATTTAGAAGTAACTCTG; TTTTTAAAATTTCAATGTTAGGAAGAAAATCCCTTAAATTTGAAATAGCGGAATATTCTC; TCATATGAAAAAACTGTGTCCCAGTGTTATTCTTCAACTTAGAAATTAGGCAAAGTATTT; TAGTACTATTTCTGCTTACATATTGTTTTGTATGAATGTTTTAAGTATGAATAATATTTT; GGTTTTTAGTGTGCATGCTAATATAAAAATATGGATTTTTTTCTCCTACACTGACTTTGT; ACACTTTCCAGGCCCGAAGAATAAAAAACTTTTAAAATATCCTGTGTTCACTAGCAAACC; TTGGAACGATAAAAAAAGGTAGTACCTACATAAGAAATTTTACATTGAAATTACAATTGT; ATGTTTTTCAGGAGTTAAAGTGATTCAATTTTTCAATATTCAAACACAAAATAGTAAATT; TTTATTTACTATAGCACAATTCAAGTATTCTCTTAAATTTATATTTAGTCAACTAAAACA; TGACAGTGACTTTATAAGAGCTTTCCTTTTAAAAGGTTGCTGTTATAAAAAAGTATGACT; CTATTACTCTAAATGCTTAGAATAAGTGGAAAGAACAAATGACAGTAGACAAAAACCTTT; ATGAGTGAGTCATCAACACATAAAAGGTTGCACACCTCAGTAATAGGACATGTGAAGGTA; ACATCCCAAAAGTATTTGAACTCATGTAAACAAAGACTATGGTATTTCCTAGGGAGAAAA; TGTNCTTCCCAGATAAAATATATTTCAACGGCTGTGACTTTTAACCATGTATTAGTTGGA; TACCCN;
• Restriction Sites: NotI-NotI
• ACCN: NM_002583
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002583.2, NP_002574.2
• RefSeq Size: 1967 bp
• RefSeq ORF: 1023 bp
• Locus ID: 5074
• UniProt ID: Q96IZ0
• Cytogenetics: 12q21.2
• Protein Families: Druggable Genome, Transcription Factors
• Summary: This gene encodes a tumor suppressor protein that selectively induces apoptosis in cancer cells through intracellular and extracellular mechanisms. The intracellular mechanism involves the inhibition of pro-survival pathways and the activation of Fas-mediated apoptosis, while the extracellular mechanism involves the binding of a secreted form of this protein to glucose regulated protein 78 (GRP78) on the cell surface, which leads to activation of the extrinsic apoptotic pathway. This gene is located on the unstable human chromosomal 12q21 region and is often deleted or mutated different tumors. The encoded protein also plays an important role in the progression of age-related diseases. [provided by RefSeq, Aug 2017]