HDJ2 (DNAJA1) (NM_001539) Human Untagged Clone

SKU SC110925

DNAJA1 (untagged)-Human DnaJ (Hsp40) homolog, subfamily A, member 1 (DNAJA1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HDJ2
• Synonyms: DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2; HSPF4; NEDD7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110925 sequence for NM_001539 edited (data generated by NextGen Sequencing); ATGGTGAAAGAAACAACTTACTACGATGTTTTGGGGGTCAAACCCAATGCTACTCAGGAA; GAATTGAAAAAGGCTTATAGGAAACTGGCCTTGAAGTACCATCCTGATAAGAACCCAAAT; GAAGGAGAGAAGTTTAAACAGATTTCTCAAGCTTACGAAGTTCTCTCTGATGCAAAGAAA; AGGGAATTATATGACAAAGGAGGAGAACAGGCAATTAAAGAGGGTGGAGCAGGTGGCGGT; TTTGGCTCCCCCATGGACATCTTTGATATGTTTTTTGGAGGAGGAGGAAGGATGCAGAGA; GAAAGGAGAGGTAAAAATGTTGTACATCAGCTCTCAGTAACCCTAGAAGACTTATATAAT; GGTGCAACAAGAAAACTGGCTCTGCAAAAGAATGTGATTTGTGACAAATGTGAAGGTAGA; GGAGGTAAGAAAGGAGCAGTAGAGTGCTGTCCCAATTGCCGAGGTACTGGAATGCAAATA; AGAATTCATCAGATAGGACCTGGAATGGTTCAGCAAATTCAGTCTGTGTGCATGGAGTGC; CAGGGCCATGGGGAGCGGATCAGTCCTAAAGATAGATGTAAAAGCTGCAACGGAAGGAAG; ATAGTTCGAGAGAAGAAAATTTTAGAAGTTCATATTGACAAAGGCATGAAAGATGGCCAG; AAGATAACATTCCATGGTGAAGGAGACCAAGAACCAGGACTGGAGCCAGGCGATATTATC; ATTGTGTTAGATCAGAAGGACCATGCTGTTTTTACTCGACGAGGAGAAGACCTTTTCATG; TGTATGGACATACAGCTCGTTGAAGCACTGTGTGGCTTCCAGAAGCCAATATCTACTCTT; GACAACCGAACCATCGTCATCACCTCTCATCCAGGTCAGATTGTCAAGCATGGAGATATC; AAGTGTGTACTAAATGAAGGCATGCCAATTTATCGTAGACCATATGAAAAGGGTCGCCTA; ATCATCGAATTTAAGGTAAACTTTCCTGAGAATGGCTTTCTCTCTCCTGATAAACTGTCT; TTGCTGGAAAAACTCCTACCCGAGAGGAAGGAAGTGGAAGAGACTGATGAGATGGACCAA; GTAGAACTGGTGGACTTTGATCCAAATCAGGAAAGACGGCGCCACTACAATGGAGAAGCA; TATGAGGATGATGAACATCATCCCAGAGGTGGTGTTCAGTGTCAGACCTCTTAA; ; Clone variation with respect to NM_001539.2; 90 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001539 unedited; TTTGTATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGNAACGCTCGGTGAGAG; GCGGAGGAGCGGTAACTACCCCGGCTGCGCACAGCTCGGCGCTCCTTCCCGCTCCCTCAC; ACACCGGCCTCAGCCCGCACCGGCAGTAGAAGATGGTGAAAGAAACAACTTACTACGATG; TTTTGGGGGTCAAACCCAATGCTACTCAGGAAGAATTGAAAAAGGCTTATAGGAAACTGG; CCTTGAAGTACCATCCTGATAAGAACCCAAATGAAGGAGAGAAGTTTAAACAGATTTCTC; AAGCTTACGAAGTTCTCTCTGATGCAAAGAAAAGGGAATTATATGACAAAGGAGGAGAAC; AGGCAATTAAAGAGGGTGGAGCAGGTGGCGGTTTTGGCTCCCCCATGGACATCTTTGATA; TGTTTTTTGGAGGAGGAGGAAGGATGCAGAGAGAAAGGAGAGGTAAAAATGTTGTACATC; AGCTCTCAGTAACCCTAGAAGACTTATATAATGGTGCAACAAGACAACTGGCTCTGCAAA; AGAATGTGATTTGTGACCAATGTGAAGGTAGAGGAGGTAAGAAAGGAGCAGTAGAGTGCT; GCCCCAATTGCCGAGGTACTGGAATGCAAATAAGAATTCATCAGATAGGACCTGGAATGG; TTCAGCAAATTCAGTCTGTGTGCATGGAGTGCCAGGGCCATGGGGAGCGGATCAGTCCTA; AAGATAGATGTAAAAGCCTGCACCGGAAGGAAGATAGCTTCGAGAGGAACAAAATCTCAC; CAGTTCATTATTGACAAAGGCCATGAAAAATGGCCAGCAGGAAACCATCCCTTGGGGAGG; GAGACCAAGAACCAGGACTGGGAGCCAGCGAATTTATATTTGTGTTANATCAGAAAGAAC; ATGGCTGTTTTTACTCGACGAGAAGATAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001539 unedited; GGGACGCGCNNGGGCCCACCCCCCCCCTTTTNNNNNAANNTTTACTTAGAACCCGGCCGC; ATACNANGATCGGATTTTTTTTTTTTTTTTATATATGCGCGTATACTTCATTTAACTCTT; TATAACACTCCTCTAGTGTATTAAAACACAAACACAAGCACGTGGTGAGCATATTATGAG; AACAGAGCTTTACTCTTTCACTACTGCGCATTAAACGCCCCCAGTGAGTGTGATTCACTG; GGCCCTTAAAAGGGGTGACACTGAGCACCACCTCTGGGAGGAGGTGCATAATCCTCATAT; GCTCTTCCATTGTGGAGGGGCCGCCTTTCCTGAGTTGGATCAAAGACCACCACATCTACT; TGGGCCATCTCATAAGACTCTTTCACTTTCTTTCTCTCGGGGAGGAGAGTTTCCACCACA; GACACTGTATCAGGAGAGAGAAAGCCCTTCTCAGGAGAGAGTACCTTAAATACGATGAGA; ATGCGACCCTCTTCATATGGGCTACAATAAATAGGGATGCCTTCATTTAGAACACACTCG; AGATCTCCATGCGCGACACTCTGACCTGTATGAGAGGTGATGACAATGGGGCTGGTGTCA; AAAGAGTATATTGGGTTCTGGAAACCACACAGAGCTTTAACGAGCTGTGTGTGCATACAC; ACGAGAAGGTGTTCTCCTCTTCGAGAAAAAACACAATGGGGCTTCTGAGCTATCACACTG; AGAATATCGCCTGTGTCCACACCTGTGGCTTGGGCTCCTTCACCACGGAATGTGATATTT; CTGGGCACATTTCATGCGCTCTGTGAATATGAAACTCCTATAAAATCTTTCTCTCTAAAT; ATATTCCTTTTCGCTGCAGCATTTTACACATATATTTATGAGAGAGCCGCTCCCCATGTG; CCCTGGACTCCATGTGCACAGACTGAATATGCTGAAACATCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_001539
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001539.2, NP_001530.1
• RefSeq Size: 1538 bp
• RefSeq ORF: 1194 bp
• Locus ID: 3301
• UniProt ID: P31689
• Cytogenetics: 9p21.1
• Domains: DnaJ, DnaJ_C, DnaJ_CXXCXGXG
• Protein Families: Druggable Genome
• Summary: This gene encodes a member of the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus. Several pseudogenes of this gene are found on other chromosomes. [provided by RefSeq, Sep 2015]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).