FBXO11 (NM_025133) Human Untagged Clone

SKU SC110924

FBXO11 (untagged)-Human F-box protein 11 (FBXO11), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: FBXO11
• Synonyms: FBX11; IDDFBA; PRMT9; UBR6; UG063H01; VIT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110924 sequence for NM_025133 edited (data generated by NextGen Sequencing); ATGGTTGCAGAAGAATCAGGTCCTGGTGCACAAAATAGTCCATACCAACTTCGTAGAAAA; ACTCTTTTGCCGAAAAGAACAGCGTGTCCCACAAAGAACAGTATGGAGGGCGCCTCAACT; TCAACTACAGAAAACTTTGGTCATCGTGCAAAACGTGCAAGAGTGTCTGGAAAATCACAA; GATCTATCAGCAGCACCTGCTGAACAGTATCTTCAGGAGAAACTGCCAGATGAAGTGGTT; CTAAAAATCTTCTCTTACTTGCTGGAACAGGATCTTTGTAGAGCAGCTTGTGTATGTAAA; CGCTTCAGTGAACTTGCTAATGATCCAATTTTGTGGAAACGATTATATATGGAAGTATTT; GAATATACTCGCCCTATGATGCATCCTGAACCTGGAAAATTCTACCAGATTAATCCAGAA; GAGTATGAACATCCAAATCCCTGGAAAGAGAGTTTCCAGCAGTTGTATAAAGGTGCACAT; GTAAAGCCAGGATTTGCTGAACATTTCTACAGTAACCCTGCAAGATATAAAGGAAGAGAA; AATATGTTGTATTATGATACTATTGAAGATGCCCTTGGTGGGGTACAAGAGGCTCATTTT; GATGGACTTATCTTTGTTCATTCTGGAATATATACTGATGAATGGATATATATTGAATCT; CCAATCACCATGATTGGTGCAGCACCTGGGAAAGTGGCAGACAAAGTTATAATTGAAAAC; ACTAGAGATTCAACCTTCGTTTTTATGGAAGGCTCTGAAGATGCTTATGTTGGATATATG; ACAATAAGGTTTAACCCTGATGACAAATCTGCACAACACCACAATGCACACCACTGCTTA; GAGATTACAGTAAATTGTAGCCCTATTATTGATCACTGTATCATCCGAAGTACATGTACA; GTTGGTTCTGCAGTATGTGTTAGTGGTCAAGGAGCATGTCCCACCATCAAGCACTGTAAC; ATCAGTGACTGTGAAAATGTTGGACTATATATAACAGATCATGCACAGGGAATATATGAG; GATAATGAAATTTCCAATAATGCGTTAGCTGGGATTTGGGTTAAAAATCATGGAAACCCA; ATTATTAGACGGAATCATATTCATCATGGACGTGATGTTGGTGTGTTCACATTTGATCAT; GGCATGGGTTACTTTGAAAGTTGCAATATACACAGAAATAGGATAGCAGGCTTTGAAGTA; AAAGCCTATGCTAACCCTACAGTGGTTCGATGTGAAATTCACCATGGGCAGACTGGAGGA; ATATATGTCCATGAAAAAGGAAGAGGACAATTCATAGAGAATAAAATCTATGCAAACAAC; TTTGCAGGTGTATGGATTACCTCAAATAGTGACCCAACAATAAGGGGAAATTCTATATTT; AATGGAAATCAAGGAGGAGTTTACATCTTTGGTGATGGACGAGGCCTTATTGAAGGAAAT; GACATTTATGGCAATGCATTAGCAGGAATTCAAATTAGGACAAACAGTTGTCCAATTGTT; CGGCATAACAAAATTCATGATGGCCAGCATGGTGGGATTTATGTGCATGAAAAGGGACAA; GGAGTAATAGAAGAGAATGAAGTTTATAGTAACACTCTAGCTGGAGTCTGGGTGACAACT; GGCAGCACTCCAGTACTGAGAAGAAACCGGATACACAGTGGCAAGCAGGTTGGTGTTTAT; TTTTATGACAATGGACATGGAGTGCTAGAAGACAATGATATCTATAATCATATGTATTCA; GGGGTTCAGATAAGGACTGGAAGCAACCCCAAAATTAGACGCAACAAAATCTGGGGAGGA; CAGAATGGTGGAATTCTAGTTTATAATTCTGGTCTAGGCTGTATAGAAGACAATGAAATA; TTTGACAATGCAATGGCTGGAGTCTGGATTAAGACAGATAGTAATCCTACACTAAGAAGA; AATAAAATCCATGATGGAAGAGATGGTGGCATCTGTATATTTAATGGGGGTCGAGGTCTC; CTTGAAGAAAATGATATTTTCAGGAATGCTCAAGCAGGTGTTCTCATCAGCACTAATAGT; CATCCAATCTTAAGGAAAAACAGAATATTTGATGGATTTGCCGCAGGTATTGAAATTACA; AATCACGCAACTGCAACACTAGAAGGCAATCAGATTTTTAACAACCGGTTTGGAGGCTTA; TTTTTAGCATCTGGTGTTAATGTGACAATGAAAGATAACAAAATAATGAACAATCAAGAT; GCCATAGAAAAGGCTGTTAGTAGAGGCCAATGTTTATATAAAATATCAAGTTATACCAGC; TATCCCATGCATGATTTCTACAGATGTCATACTTGTAACACCACAGATCGAAATGCCATA; TGTGTGAACTGCATTAAGAAGTGCCATCAGGGACATGATGTAGAGTTTATTAGACATGAT; AGGTTTTTCTGTGACTGTGGTGCTGGAACACTGTCTAATCCTTGTACATTAGCTGGTGAG; CCTACACATGATACAGATACACTATATGACTCTGCTCCACCTATAGAATCTAATACATTG; CAGCACAACTGA; ; Clone variation with respect to NM_025133.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025133 unedited; AGTATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCAGGAGCGG; ACAACGTCGGCGAGCGGGATGATGATGTGCCTGCAGATATGGTTGCAGAAGAATCAGGTC; CTGGTGCACAAAATAGTCCATACCAACTTCGTAGAAAAACTCTTTTGCCGAAAAGAACAG; CGTGTCCCACAAAGAACAGTATGGAGGGCGCCTCAACTTCAACTACAGAAAACTTTGGTC; ATCGTGCAAAACGTGCAAGAGTGTCTGGAAAATCACAAGATCTATCAGCAGCACCTGCTG; AACAGTATCTTCAGGAGAAACTGCCAGATGAAGTGGTTCTAAAAATCTTCTCTTACTTGC; TGGAACAGGATCTTTGTAGAGCAGCTTGTGTATGTAAACGCTTCAGTGAACTTGCTAATG; ATCCAATTTTGTGGAAACGATTATATATGGAAGTATTTGAATATACTCGCCCTATGATGC; ATCCTGAACCTGGAAAATTCTACCAGATTAATCCAGAAGAGTATGAACATCCAAATCCCT; GGAAAGAGAGTTTCCAGCAGTTGTATAAAGGTGCACATGTAAAGCCAGGATTTGCTGAAC; ATTTCTACAGTAACCCTGCAAGATATAAAGGAAGAGAAAATATGTTGTATTATGATACTA; TTGAAGATGCCCTTGGTGGGAGTACAAGANGCTCATTNTGATGGACTTATCTTTGTTCAT; TCTGGGATATATACTGATGAATGGATATATATTGAATCTNNCATCACCATGATGGNTGCA; GCACTGGNGAAGTGGCAGANAAGTTATAATTGAAACACTAAGATCAACCTTCGTTTTTAG; GAAGGCTCTGAGAAGCTATGTTGGAATATACAATAAGGTTAACCTGT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_025133 unedited; CTTGGACCGCGGCCGCATCTAAATACGGTTTTTTTTTTTTTTTTTTATGATCTAATAAAC; TTTATCTTTTTAAAAATGACCATTTTTCCATTTTCTTTCTAGGAAATTAAACCCTTTTAA; TTCTTATCTACCTTCTACATAATGGTTATTGAATACTCCACAATATATTAAGTCTAGATG; TTATGGTACATGCATACACTTTCAGGCTGTTTTATACCCACTGTCACCAATACACATAAA; TGGGGGAGGAAAAGCTATGAAACTGTATAGGGCTGTATATATACTTGTCTCAGCTTAATG; CAGGAAATTGGTTTAATTTCCAGCAGTTTTGTCTAAACTGTTCAAAAAAAAACTATGAAC; AGAGTTCAAATACAGGACTGTTTGTTTTGAAGAGACTTTCTAAAGTGTACTTAAAACATA; GTAGTTTTTTACCTTTCACAAAACTGAGTTACAAGAATACTTTTGTTTTACAGTGCATCC; CTTCCTAGGAAGTCTCATTAAAACACTCACTTTTTCTAGGGGTGATTTTGAATGCTGCAC; AGGGAAGGGAAGGAAATAATAGTCTTAACTTTTCTTAAAGGATACCAGAAACATTGCTGG; ATATAATTTAAGATTAGAGCTTTCTCTTTCATAGAAAGAACGTACATACTGNGACATGAG; TACAGTTACAGCAAGTCTAGGTGTGCTAACAAAACAGGGCACATTCAAGTACAGTAAGAT; TCTACTTGAAATTAAAAACAAACTACATGAGATTAAAGCATTAAAATATATTTCTCAATC; TGAATACATGTCTAAAAAAAAATCANAAGGAACGCAGAAGTGCTAGCTCACATTTTTACC; ATATTACAAAAGCAATGGGTACCCATGTCCCATAAAGCAGCAACAAAGCTGCTTGTCTAT; TGAGATACTACCGCANATTNACTGCCTTCATGCTCATTGTAAAACCCAGNCTTTCAGAGT; GGTATCTGACCAAATGCAGTTATTTCTTCCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_025133
• Insert Size: 3800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_025133.3, NP_079409.3
• RefSeq Size: 3951 bp
• RefSeq ORF: 2532 bp
• Locus ID: 80204
• UniProt ID: Q86XK2
• Cytogenetics: 2p16.3
• Domains: CASH, PbH1
• Protein Families: Druggable Genome
• Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. This gene is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis media with effusion and recurrent otitis media (COME/ROM), a hearing loss disorder, and the knockout of the homologous mouse gene results in the deaf mouse mutant Jeff (Jf), a single gene model of otitis media. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2010]; Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.