Cystatin C (CST3) (NM_000099) Human Untagged Clone

CAT#: SC110862

CST3 (untagged)-Human cystatin C (CST3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cystatin C
• Synonyms: ARMD11; HEL-S-2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110862 sequence for NM_000099 edited (data generated by NextGen Sequencing)
  ATGGCCGGGCCCCTGCGCGCCCCGCTGCTCCTGCTGGCCATCCTGGCCGTGGCCCTGGCC
  GTGAGCCCCGCGGCCGGCTCCAGTCCCGGCAAGCCGCCGCGCCTGGTGGGAGGCCCCATG
  GACGCCAGCGTGGAGGAGGAGGGTGTGCGGCGTGCACTGGACTTTGCCGTCGGCGAGTAC
  AACAAAGCCAGCAACGACATGTACCACAGCCGCGCGCTGCAGGTGGTGCGCGCCCGCAAG
  CAGATCGTAGCTGGGGTGAACTACTTCTTGGACGTGGAGCTGGGCCGAACCACGTGTACC
  AAGACCCAGCCCAACTTGGACAACTGCCCCTTCCATGACCAGCCACATCTGAAAAGGAAA
  GCATTCTGCTCTTTCCAGATCTACGCTGTGCCTTGGCAGGGCACAATGACCTTGTCGAAA
  TCCACCTGTCAGGACGCCTAG
  
  Clone variation with respect to NM_000099.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000099 unedited
  GTATACGACTCCTATAGGCGGCCGCGACATTCGCACGAGGCTCGGTATCGCAGCGGGTCC
  TCTCTATCTAGCTCCAGCCTCTCGCCTGCGCCCCACTCCCCGCGTCCCGCGTCCTAGCCG
  ACCATGGCCGGGCCCCTGCGCGCCCCGCTGCTCCTGCTGGCCATCCTGGCCGTGGCCCTG
  GCCGTGAGCCCCGCGGCCGGCTCCAGTCCCGGCAAGCCGCCGCGCCTGGTGGGAGGCCCC
  ATGGACGCCAGCGTGGAGGAGGAGGGTGTGCGGCGTGCACTGGACTTTGCCGTCGGCGAG
  TACAACAAAGCCAGCAACGACATGTACCACAGCCGCGCGCTGCAGGTGGTGCGCGCCCGC
  AAGCAGATCGTAGCTGGGGTGAACTACTTCTTGGACGTGGAGCTGGGCCGAACCACGTGT
  ACCAAGACCCAGCCCAACTTGGACAACTGCCCCTTCCATGACCAGCCACATCTGAAAAGG
  AAAGCATTCTGCTCTTTCCAGATCTACGCTGTGCCTTGGCAGGGCACAATGACCTTGTCG
  AAATCCACCTGTCAGGACGCCTAGGGGTCTGTACCGGGCTGGCCTGTGCCTATCCCTCTA
  TCNCACACCCTCCCACCCCCTGTATTCCCACCCCTGGACTGGTGGCCCCCGGCCTGGGGG
  AAGTTCTCCCCATTGTCCCTGCACCANGAGACAGACAGAGAAGGCAGCAGGCGGGNCCTT
  TGNTGCTCAGCAGGGGGCTCTGCCCTCCCTCCTTCCTTTCTTGCTCTCATAGCCCCGGGG
  TGCGGTGCATACACCCCCCACCTCCTGCATAAAATAGTAGCATCGGCAAAAAAAAAAAAA
  AACTCACTCTAAATTGCGGCCGCGGTCATAACTGTTTCTGAAAAAACCCGGTGGGGATCC
  TGGGGACCCTCCCAAGCCTTTCTGGCCTGGGAAGGCCATTCCAGGCCCCCACCTGGCTAA
  AAAAAAAGCAATTTGCGACAGGGCTTATATGGGGGGGGGGGGGGGGGGGGNGGACACAAC
  CACACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000099 unedited
  AGCTATGGACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCCGATGC
  TACTATTTTATTGCAGGAGGTGGGGGTGTATGCACCGCACACCGGGGCTATGAGAAGCAA
  GAAGGAAGGAGGGAGGGCAGAGCCCCTTGCTGAGCAACAAAGGCCGCCTGCTGCCTTCTC
  TGTCTGTCTCCTGGTGCAGGCACATGGGGAGACCTTCCCCAAGGCAGGGGCCACCAGTCC
  AGGGGTGGGAATACAGGGGGTGGGAGGTGTGCATAAGAGGTGATAGGCACAGGCCAGCCC
  GGTACAGACCCCTAGGCGTCCTGACAGGTGGATTTCGACAAGGTCATTGTGCCCTGCCAA
  GGCACAGCGTAGATCTGGAAAGAGCAGAATGCTTTCCTTTTCAGATGTGGCTGGTCATGG
  AAGGGGCAGTTGTCCAAGTTGGGCTGGGTCTTGGTACACGTGGTTCGGCCCAGCTCCACG
  TCCAAGAAGTAGTTCACCCCAGCTACGATCTGCTTGCGGGCGCGCACCACCTGCAGCGCG
  CGGCTGTGGTACATGTCGTTGCTGGCTTTGTTGTACTCGCCGACGGCAAAGTCCAGTGCA
  CGCCGCACACCCTCCTCCTCCACGCTGGCGTCCATGGGGCCTCCCACCAGGCGCGGCGGC
  TTGCCGGGACTGGAGCCGGCCGCGGNGCTCACGGNCCAGGCCACGGCCAGGATGGCCCAG
  CAGAGCCAGCGGGCGCGCAGGGGCCCGGCCATGGTCGGCTAGGACGCCGGACCCNGNGAG
  TGGGGCCCAAGCGAAAGCCTGAANCTANATANAGAGGACCCGCTGCGATACGGAGCCTCG
  TGCCGAATTCCGGNCCGCCCTAAGTGAGTCGTATACAAAATCTGACGNTCACTAACGAGC
  TCTGCTATATAGACCTCCCACGTCACGCCTAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000099
• Insert Size: 820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000099.2, NP_000090.1
• RefSeq Size: 818 bp
• RefSeq ORF: 441 bp
• Locus ID: 1471
• UniProt ID: P01034
• Cytogenetics: 20p11.21
• Domains: CY
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Gene Summary: The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein. [provided by RefSeq, Nov 2014]
  Transcript Variant: This variant (1) differs in the 3' UTR compared to variant 2. Variants 1 and 2 encode the same protein.