TAGAP (NM_152133) Human Untagged Clone

SKU SC110772

TAGAP (untagged)-Human T-cell activation RhoGTPase activating protein (TAGAP), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: TAGAP
• Synonyms: ARHGAP47; FKSG15; IDDM21; TAGAP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_152133 edited; ATGGGTGCTCTGGAGATGCAGGACGAGGAGGACAGAATCGAGGCCCTGAAACAGGTTGCA; GATAAGCTCCCCCGGCCCAACCTCCTGCTACTCAAGCACTTGGTCTATGTGCTGCACCTC; ATCAGCAAGAACTCTGAGGTGAACAGGATGGACTCCAGCAATCTGGCCATCTGCATTGGA; CCCAACATGCTCACCCTGGAGAATGACCAGAGCCTGTCATTTGAAGCCCAGAAGGACCTG; AACAACAAGGTGAAGACACTGGTGGAATTCCTCATTGATAACTGCTTTGAAATATTTGGG; GAGAACATTCCAGTGCATTCCAGTATCACTTCTGATGACTCCCTGGAGCACACTGACAGT; TCAGATGTGTCGACCCTGCAGAATGACTCAGCCTACGACAGCAACGACCCTGATGTGGAA; TCCAACAGCAGCAGTGGCATCAGCTCTCCCAGCAGGCAGCCCCAGGTGCCCATGGCCACA; GCTGCTGGCTTGGATAGCGCGGGCCCACAGGATGCCCGAGAGGTCAGCCCAGAGCCCATT; GTGAGCACCGTGGCCAGGCTGAAAAGCTCCCTCGCACAGCCCGATAGGAGATACTCAGAG; CCCAGCATGCCATCCTCCCAGGAGTGCCTCGAGAGCCGGGTGACAAACCAAACACTAACA; AAGAGTGAAGGGGACTTCCCCGTGCCCCGGGTAGGCTCTCGTTTGGAAAGTGAGGAGGCT; GAAGACCCATTTCCAGAGGAGGTCTTCCCTGCAGTGCAAGGCAAAACCAAGAGGCCGGTG; GACCTGAAGATCAAGAACTTGGCCCCGGGTTCGGTGCTCCCGCGGGCACTGGTTCTCAAA; GCCTTCTCCAGCAGCTCGCTGGACGCGTCCTCTGACAGCTCGCCCGTGGCTTCTCCTTCC; AGTCCCAAAAGAAATTTCTTCAGCAGACATCAGTCTTTCACCACAAAGACAGAGAAAGGC; AAGCCCAGCCGAGAAATTAAAAAGCACTCCATGTCTTTCACCTTTGCCCCTCACAAAAAA; GTGCTGACCAAAAACCTCAGCGCGGGCTCTGGGAAATCGCAAGACTTTACCAGGGACCAC; GTCCCGAGGGGTGTCAGAAAGGAAAGCCAGCTTGCCGGCCGAATCGTGCAGGAAAATGGG; TGTGAAACCCACAACCAAACAGCCCGCGGCTTCTGCCTGAGACCCCACGCCCTCTCGGTG; GATGATGTGTTCCAGGGAGCTGACTGGGAGAGGCCTGGAAGCCCACCCTCTTATGAAGAG; GCCATGCAGGGCCCGGCAGCCAGACTAGTGGCCTCCGAGAGCCAGACCGTGGGGAGCATG; ACGGTGGGGAGCATGAGGGCGAGGATGCTGGAGGCGCACTGCCTCCTACCCCCTCTTCCA; CCTGCTCACCACGTAGAGGACTCAAGACACAGGGGCAGCAAAGAGCCACTCCCTGGCCAC; GGACTCTCTCCCCTGCCTGAGCGATGGAAACAGAGCAGAACTGTCCATGCTTCTGGGGAC; TCTCTGGGGCACGTGTCTGGCCCAGGGAGACCTGAGCTCCTCCCGCTGAGGACCGTCTCC; GAGTCCGTGCAGAGGAATAAGCGGGACTGTCTCGTGCGACGATGTAGCCAGCCGGTCTTT; GAGGCTGACCAATTCCAATATGCCAAAGAATCGTATATTTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152133 unedited; NGGGGAGTTCAAAATTGTATACGACTCACTATAGGCGGCCGCGAAATTCGCACGAGGTGA; CAGTGACACACTCCCCANACCCATCCAGGACATTCTCACTATTCTATGCCTTAAAGGCCC; TTCAACGGAAGGGATATTCAGGAGAGCAGCCAACGAGAAAGCCCGTAAGGAGCTGAAGGA; GGAGCTCAACTCTGGGGATGCGGTGGATCTGGAGAGGCTCCCCGTGCACCTCCTCGCTGT; GGTCTTTAAGGACTTCCTCAGAAGTATCCCCCGGAAGCTACTTTCAAGCGACCTCTTTGA; GGAGTGGATGGGTGCTCTGGAGATGCAGGACGAGGAGGACAGAATCGAGGCCCTGAAACA; GGTTGCAGATAAGCTCCCCCGGCCCAACCTCCTGCTACTCAAGCACTTGGTCTATGTGCT; GCACCTCATCAGCAAGAACTCTGAGGTGAACAGGATGGACTCCAGCAATCTGGCCATCTG; CATTGGACCCAACATGCTCACCCTGGAGAATGACCAGAGCCTGTCATTTGAAGCCCAGAA; GGACCTGAACAACAAGGTGAAGACACTGGTGGAATTCCTCATTGATAACTGCTTTGAAAT; ATTTGGGGAGACATTTTCCAGTGCATTCCAGTATCACTTCTGATGACTCCCTGGAGCACA; CTGACAGTTCAGATGTGTCGACCCTGCAGAATGACTCAGCCTACGACAGCAACGACCCTG; ATGTGGAATCCAACAGCAGCAGTGGCATCAGCTCTCCCAGCAGGCAGNCCCAGGTGCCCA; TGGCCACAGCTGCTGGCTTGGATAGCGCGGGCCCACAGATGCCCCGANAGGTCAGCCCAG; AGCCCATTGTGAGCACCGTGGGCCAGCTGAAAAGCTCCTCGCACANCCGATAGGAGAACT; CAGACCCAGCATGCCATCTNCCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152133 unedited; NGGTTCGACTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTAC; TTTCAAATAAGGAAGGCAATTTATTGTAGTGAAATTACATTGAAGTTTTATATATGACCC; CCAATGCATATAAAATCATTTACCTCCACATTATAGGATAGGATATCATGAACTACAAAG; ATACTCAAAGTGCTATAGATGAGTTAATATTGATGTAATACATTTTCATTAATCTACCCA; CATTTAATTGGATGATTGAAAGGACACGTTGAAGTGTTTTTCATGATTTTTGCTACGGCC; CCAGCTTTTACTGGAGTTTTTAATGCCTTTGATGACTCCAGACACTCAACTCTGTTCCTT; GCAGTCTGTTTCTTTCTTCTATGGTGGTGATTAGTTATTGCAAGACTGCTTATATAGAGT; AATATATAGTTTACTCTGGTATGGAGTAAACTTATACCAGAGATTTAAACATTTCTCCCT; AAATGGAGTTTGTGGAAGATGTGAAAATTGGCATGACATTTGAGGCCACATTAAAAAAGC; GAAGATAATTTTTAAACACTAACCAAGAGAAAAGTGCCCCAGGAAGCAAGGAACAGCAGT; GTACTTCACTGCAAGTTCAAAACGTGTGCAAGGATTATTAGACATCCTTTGCACCTAACA; CCTTATCTATAATACAATTGATACATTTTTACATATTGGACAAATCCAGGTACACCGAGA; CTATTTGATGCGCCCTGGCGCTGGTGTAACTATTCTCACTGAGGGAGGGCTTTCCCAACT; TTCTCAAGGAGCTTATTCCAGACCTTTTAAAAACATCTACAGGGCATTCCTTTACAAGTC; TTATATTTACACATAGACCAACCTATGGCATGGGCGTATGGGCCCCCCTCCCTAAAAAAC; CAATACCTTTGGCCTAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_152133
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152133.1, NP_687034.1
• RefSeq Size: 3196 bp
• RefSeq ORF: 1662 bp
• Locus ID: 117289
• UniProt ID: Q8N103
• Cytogenetics: 6q25.3
• Summary: This gene encodes a member of the Rho GTPase-activator protein superfamily. The encoded protein may function as a Rho GTPase-activating protein. Alterations in this gene may be associated with several diseases, including rheumatoid arthritis, celiac disease, and multiple sclerosis. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2013]; Transcript Variant: This variant (1) lacks an exon in the coding region compared to variant 2, resulting in the use of a downstream start codon. The encoded isoform (a) has a shorter N-terminus compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.