ASRGL1 (NM_025080) Human Untagged Clone

SKU SC110613

ASRGL1 (untagged)-Human asparaginase like 1 (ASRGL1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: ASRGL1
• Synonyms: ALP; ALP1; CRASH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110613 sequence for NM_025080 edited (data generated by NextGen Sequencing); ATGAATCCCATCGTAGTGGTCCACGGCGGCGGAGCCGGTCCCATCTCCAAGGATCGGAAG; GAGCGAGTGCACCAGGGCATGGTCAGAGCCGCCACCGTGGGCTACGGCATCCTCCGGGAG; GGCGGGAGCGCCGTGGATGCCGTAGAGGGAGCTGTCGTCGCCCTGGAAGACGATCCCGAG; TTCAACGCAGGTTGTGGGTCTGTCTTGAACACAAATGGTGAGGTTGAAATGGATGCTAGT; ATCATGGATGGAAAAGACCTGTCTGCAGGAGCAGTGTCCGCAGTCCAGTGTATAGCAAAT; CCCATTAAACTTGCTCGGCTTGTCATGGAAAAGACACCTCATTGCTTTCTGACTGACCAA; GGCGCAGCGCAGTTTGCAGCAGCTATGGGGGTTCCAGAGATTCCTGGAGAAAAACTGGTG; ACAGAGAGAAACAAAAAGCGCCTGGAAAAAGAGAAGCATGAAAAAGGTGCTCAGAAAACA; GATTGTCAAAAAAACTTGGGAACCGTGGGTGCTGTTGCCTTGGACTGCAAAGGGAATGTA; GCCTACGCAACCTCCACAGGCGGTATCGTTAATAAAATGGTCGGCCGCGTTGGGGACTCA; CCGTGTCTAGGAGCTGGAGGTTATGCCGACAATGACATCGGAGCCGTCTCAACCACAGGG; CATGGGGAAAGCATCCTGAAGGTGAACCTGGCTAGACTCACCCTGTTCCACATAGAACAA; GGAAAGACGGTAGAAGAGGCTGCGGACCTATCGTTGGGTTATATGAAGTCAAGGGTTAAA; GGTTTAGGTGGCCTCATCGTGGTTAGCAAAACAGGAGACTGGGTGGCAAAGTGGACCTCC; ACCTCCATGCCCTGGGCAGCCGCCAAGGACGGCAAGCTGCACTTCGGAATTGATCCTGAC; GATACTACTATCACCGACCTTCCCTAA; ; Clone variation with respect to NM_025080.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025080 unedited; TGTCAGCATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGGTGACCAGA; GCGGTGGCGGGCTGAGCGGTTTCGAGCCGGCGTCGGGGAGCGGCGGTACCGGGCGGCTGC; GGGGCTGGCTCGACCCAGCTTGAGGTCTCGGCGTCCGCGTCCTGCGGTGCCCTGGGATCC; GCCGACATGAATCCCATCGTAGTGGTCCACGGCGGCGGAGCCGGTCCCATCTCCAAGGAT; CGGAAGGAGCGAGTGCACCAGGGCATGGTCAGAGCCGCCACCGTGGGCTACGGCATCCTC; CGGGAGGGCGGGAGCGCCGTGGATGCCGTAGAGGGAGCTGTCGTCGCCCTGGAAGACGAT; CCCGAGTTCAACGCAGGTTGTGGGTCTGTCTTGAACACAAATGGTGAGGTTGAAATGGAT; GCTAGTATCATGGATGGAAAAGACCTGTCTGCAGGAGCAGTGTCCGCAGTCCAGTGTATA; GCAAATCCCATTAAACTTGCTCGGCTTGTCATGGAAAAGACACCTCATTGCTTTCTGACT; GACCAAGGCGCAGCGCAGTTTGCAGCAGCTATGGGGGTTCCAGAGATTCCTGGAGAAAAA; CTGGTGACAGAGAGAAACAAAAAGCGCCTGGAAAAAGAGAAGCATGAAAAAGGTGCTCAG; AAAACAGATTGTCAAAAAAACTTGGGAACCGTGGGTGCTGTTGCCTTGGACTGCAAAGGG; AATGTAGCCTACGCAACCTCCACAGGCGGTATCGTTAATAAAATGGTCGGCCGCGTTGGN; GACTCACCGTGTCTAGGAGCTGGNNAGTTATGCCCGACATGACATCGNAGCCGTCTCAAC; CACAGGCATGGGGGAAGCATCCTGAANGGGAACCTGGCTAGACTCACCCTGTTCCCATAG; AACAAGGAAGACN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_025080 unedited; TTAGCTTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCTTTTTT; TTTTGATACAGGGGCCTGGCTCTGTTGCCCAGGCTGGAGTGCAGTGGAGTAATCATGGCT; CACCTCAGCTTTGACCTCCAGGGCTCAAGTAATCCTCCTAATACGGGCATTTCTCTCATC; GCTTCAGAACCCGCCCCACAACCAAACATTCAGATGCTTATTAAGGCAACAAAACAAGTG; ACAGACGGGACAATTTTTCCAATTCTAGATCTAATTGATTAGGCTATGTCTCATGAGGAG; ACTGTACTTGACCTCTAAGCTAGCATCTGGAATACAATCTTCCAGCGGCTTAGGGAAGGT; CGGTGATAGTAGTATCGTCAGGATCAATTCCGAAGTGCAGCTTGCCGTCCTTGGCGGCTG; CCCAGGGCATGGAGGTGGAGGTCCACTTTGCCACCCAGTCTCCTGTTTTGCTAACCACGA; TGAGGCCACCTAAACCTTTAACCCTTGACTTCATATAACCCAACGATAGGTCCGCAGCCT; CTTCTACCGTCTTTCCTTGTTCTATGTGGAACAGGGTGAGTCTAGCCAGGTTCACCTTCA; GGATGCTTTCCCCATGCCCTGTGGTTGAGACGGCTCCNATGTCATTGTCGGCATAACCTC; CAGCTCCTAGACACGGGTGAGTCCCCAACGCGGCCGACCATTTTATTAACGATACCGCCT; GTGGGAGGTTTGCGTAGGCTACATTCCCTTTTGCAGTCCAAGGCACAGCACCCACGGTTC; CCAAAGTTTTTTGACNATCTGNTTTCTGAGCACTTTTTCATGCTNCTCTTTTTCAGCGCT; TTTTTGTTTCTCTTGNTCCCAGTTTTTTCCAGGATCTCTGGAACCCCATACTGCTGAACT; GCGCTGGCCTTGGCAGCAAGCAAAGAGGGGTGCTTTCTGACAGCCAGCAGTAAAGAGGGA; NTGCATCACTGACTGGGACTGTCTGGAAAAGGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_025080
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_025080.2, NP_079356.2
• RefSeq Size: 1332 bp
• RefSeq ORF: 927 bp
• Locus ID: 80150
• UniProt ID: Q7L266
• Cytogenetics: 11q12.3
• Domains: Asparaginase_2
• Protein Families: Protease
• Summary: Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.