BACE2 (NM_012105) Human Untagged Clone

CAT#: SC110343

BACE2 (untagged)-Human beta-site APP-cleaving enzyme 2 (BACE2), transcript variant a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BACE2
• Synonyms: AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13; CEAP1; DRAP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_012105, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCGCACTGGCCCGGGCGCTGCTGCTGCCTCTGCTGGCCCAGTGGCTCCTGCGCGCCGCCCCGGAGC
  TGGCCCCCGCGCCCTTCACGCTGCCCCTCCGGGTGGCCGCGGCCACGAACCGCGTAGTTGCGCCCACCCC
  GGGACCCGGGACCCCTGCCGAGCGCCACGCCGACGGCTTGGCGCTCGCCCTGGAGCCTGCCCTGGCGTCC
  CCCGCGGGCGCCGCCAACTTCTTGGCCATGGTAGACAACCTGCAGGGGGACTCTGGCCGCGGCTACTACC
  TGGAGATGCTGATCGGGACCCCCCCGCAGAAGCTACAGATTCTCGTTGACACTGGAAGCAGTAACTTTGC
  CGTGGCAGGAACCCCGCACTCCTACATAGACACGTACTTTGACACAGAGAGGTCTAGCACATACCGCTCC
  AAGGGCTTTGACGTCACAGTGAAGTACACACAAGGAAGCTGGACGGGCTTCGTTGGGGAAGACCTCGTCA
  CCATCCCCAAAGGCTTCAATACTTCTTTTCTTGTCAACATTGCCACTATTTTTGAATCAGAGAATTTCTT
  TTTGCCTGGGATTAAATGGAATGGAATACTTGGCCTAGCTTATGCCACACTTGCCAAGCCATCAAGTTCT
  CTGGAGACCTTCTTCGACTCCCTGGTGACACAAGCAAACATCCCCAACGTTTTCTCCATGCAGATGTGTG
  GAGCCGGCTTGCCCGTTGCTGGATCTGGGACCAACGGAGGTAGTCTTGTCTTGGGTGGAATTGAACCAAG
  TTTGTATAAAGGAGACATCTGGTATACCCCTATTAAGGAAGAGTGGTACTACCAGATAGAAATTCTGAAA
  TTGGAAATTGGAGGCCAAAGCCTTAATCTGGACTGCAGAGAGTATAACGCAGACAAGGCCATCGTGGACA
  GTGGCACCACGCTGCTGCGCCTGCCCCAGAAGGTGTTTGATGCGGTGGTGGAAGCTGTGGCCCGCGCATC
  TCTGATTCCAGAATTCTCTGATGGTTTCTGGACTGGGTCCCAGCTGGCGTGCTGGACGAATTCGGAAACA
  CCTTGGTCTTACTTCCCTAAAATCTCCATCTACCTGAGAGACGAGAACTCCAGCAGGTCATTCCGTATCA
  CAATCCTGCCTCAGCTTTACATTCAGCCCATGATGGGGGCCGGCCTGAATTATGAATGTTACCGATTCGG
  CATTTCCCCATCCACAAATGCGCTGGTGATCGGTGCCACGGTGATGGAGGGCTTCTACGTCATCTTCGAC
  AGAGCCCAGAAGAGGGTGGGCTTCGCAGCGAGCCCCTGTGCAGAAATTGCAGGTGCTGCAGTGTCTGAAA
  TTTCCGGGCCTTTCTCAACAGAGGATGTAGCCAGCAACTGTGTCCCCGCTCAGTCTTTGAGCGAGCCCAT
  TTTGTGGATTGTGTCCTATGCGCTCATGAGCGTCTGTGGAGCCATCCTCCTTGTCTTAATCGTCCTGCTG
  CTGCTGCCGTTCCGGTGTCAGCGTCGCCCCCGTGACCCTGAGGTCGTCAATGATGAGTCCTCTCTGGTCA
  GACATCGCTGGAAATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012105 unedited
  CCTCGTTTTGTAAACGACTTCATATAGGCGGCCGACGAATTCGCACGAGGTCCTCCCCGC
  CGCCGCCGGTCCCGGTGCGCGCCCATCCCTGCCCGCAGCCCCGCGCGCCGGCCGAGTCGC
  TGAGCCGCGGCTGCCGGACGGGACGGGACCGGCTAGGCTGGGCGCGCCCCCCGGGCCCCG
  CCGTGGGCATGGGCGCACTGGCCCGGGCGCTGCTGCTGCCTCTGCTGGCCCAGTGGCTCC
  TGCGCGCCGCCCCGGAGCTGGCCCCCGCGCCCTTCACGCTGCCCCTCCGGGTGGCCGCGG
  CCACGAACCGCGTAGTTGCGCCCACCCCGGGACCCGGGACCCCTGCCGAGCGCCACGCCG
  ACGGCTTGGCGCTCGCCCTGGAGCCTGCCCTGGCGTCCCCCGCGGGCGCCGCCAACTTCT
  TGGCCATGGTAGACAACCTGCAGGGGGACTCTGGCCGCGGCTACTACCTGGAGATGCTGA
  TCGGGACCCCCCCGCAGAAGCTACAGATTCTCGTTGACACTGGAAGCAGTAACTTTGCCG
  TGGCAGGAACCCCGCACTCCTACATAGACACGTACTTTGACACAGAGAGGTCTAGCACAT
  ACCGCTCCAAGGGCTTTGACGTCACAGTGAAGTACACACAAGGAAGCTGGACGGGCTTCG
  TTGGGGAAGACCTCGTCACCATCCCCAAAGGCTTCAATACTTCTTTTCTTGTCAACATTG
  CCACTATTTTTGAATCAGAGAATTTCTTTTTGCCTGGGATTAAATGGAATGGNANNTACT
  GCCTAGCTTATGCCACACTTGCCAAGCCATCAAGTTCTCTGGAGAACCTCTTCGACTCCC
  TGGTGACACAAGCAAACATCCCCCACGTTTCCTCATGCANATGTGTGGAGCCCGCTTGCC
  CCGTGCTGGATCTGGGACCACCGGAGTAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012105 unedited
  NNNNNNTTTTGGGGGGGTTTTGGAAGCTTGGAAATCAACATCAAAATACAGTGAATCTAG
  AAGCATCTGGGAGCAGAACAGAGATTGAAGACGGGTGGGCACAGGTAGAAAGCGCCACCA
  TCGATCCCGGCTGCTGCCCTGGAAATGTGATTTTCTTAATAGCTGAGTTCATGGTTGCTT
  GAGGTCAGGCCTGGCTATTCATTTCCAGCGATGTCTGACCAGAGAGGACTCATCATTGAC
  GACCTCAGGGTCACGGGGGCGACGCTGACACCGGAACGGCAGCAGCAGCAGGACGATTAA
  GACAAGGAGGATGGCTCCACAGACGCTCATGAGCGCATAGGACACAATCCACAAAATGGG
  CTCGCTCAAAGACTGAGCGGGGACACAGTTGCTGGCTACATCCTCTGTTGAGAAAGGCCC
  GGAAATTTCAGACACTGCAGCACCTGCAATTTCTGCACAGGGGCTCGCTGCGAAGCCCAC
  CCTCTTCTGGGCTCTGTCGAAGATGACGTAGAAGCCCTCCATCACCGTGGCACCGATCAC
  CAGCGCATTTGTGGATGGGGAAATGCCGAATCGGTAACATTCATAATTCAGGCCGGCCCC
  CATCATGGGCTGAATGTAAAGCTGAGGCAGGATTGTGATACGGAATGACCTGCTGGAGTT
  CTCGTCTCTCAGGTAGATGGAGATTTTAGGGAAGTAAGACCAAGGTGTTTCCGAATTCGT
  CCAGCACGCCAGCTGGGACCCAGTCCAGANACCATCAGAGAATTCTGGAATCAGAGATGC
  GCGGGCCACAGCTTCCACCACCGCATCAAACACCTTCTGGGGCATGCGCAGCAGCGTGGT
  GCCACTGTACACGATGGCCTTGTCTGCGTTATACTCTCTGCAGTCCAGATAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012105
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012105.3, NP_036237.2
• RefSeq Size: 2993 bp
• RefSeq ORF: 1557 bp
• Locus ID: 25825
• UniProt ID: Q9Y5Z0
• Cytogenetics: 21q22.2-q22.3
• Domains: asp
• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Alzheimer's disease
• Gene Summary: This gene encodes an integral membrane glycoprotein that functions as an aspartic protease. The encoded protein cleaves amyloid precursor protein into amyloid beta peptide, which is a critical step in the etiology of Alzheimer's disease and Down syndrome. The protein precursor is further processed into an active mature peptide. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (a) represents the longest transcript and encodes the longest isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.